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Preparation and application of morinda root water extract, oligosaccharides and polysaccharides

A technology of Morinda officinalis oligosaccharides and Morinda officinalis, which is applied in the fields of traditional Chinese medicine, hepatology and immunology, can solve the problems of less research on the physical and chemical properties and structural characteristics of polysaccharide components, and achieve the promotion of spleen cell proliferation and cell Effects of factor secretion, inhibition of expression, and little environmental pollution

Active Publication Date: 2018-11-06
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemistry of oligosaccharides in Morinda officinalis has been studied, but there are few studies on the physical and chemical properties and structural characteristics of polysaccharides

Method used

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  • Preparation and application of morinda root water extract, oligosaccharides and polysaccharides
  • Preparation and application of morinda root water extract, oligosaccharides and polysaccharides
  • Preparation and application of morinda root water extract, oligosaccharides and polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1: Preparation of Morinda officinalis water extract

[0072] Weigh 1 kg of decoction pieces of Morinda officinalis medicinal material, add 20L of water, soak for 1 hour and then decoct for 1.5 hours. Filter, add 20L of water to the dregs, decoct for 1 hour, and filter. Combine the filtrates and centrifuge (3000r / min×20min). The supernatant is concentrated under reduced pressure at 55-60° C., and the concentrated extract is dried and vacuum-dried to obtain a water-extracted dry extract.

Embodiment 2

[0073] Example 2: Preparation of small molecular parts and crude polysaccharides in the water extract of Morinda officinalis Morinda officinalis (1)

[0074] The original medicinal material of Morinda officinalis is crushed and passed through a 20-mesh sieve. Weigh 3 parts of Morinda officinalis root powder, each 1kg. Add 15L of distilled water for extraction at room temperature (20°C), 60°C and 100°C, respectively. Among them, 24 hours of leaching at room temperature, 4 hours of leaching at 60°C, 1.5 hours of decocting at 100°C, and stirring during the leaching. The extract was filtered, and the filter residues were extracted again under the same conditions. The two extracted water extracts were combined and centrifuged (3000r / min×20min), and the supernatants obtained at different temperatures were respectively concentrated to 1.5L under reduced pressure at 50-55°C to obtain a water extract concentrate.

[0075] Add 4 times the volume of 95% ethanol to the Morinda officina...

Embodiment 3

[0077] Embodiment 3: the preparation of the crude polysaccharide of Morinda officinalis Radix Morinda officinalis (two)

[0078] The original medicinal material of Morinda officinalis is crushed and passed through a 20-mesh sieve. Weigh 1kg of Morinda officinalis root powder, add 15L of distilled water, stir and extract at 60°C for 5 hours, then filter. Add 15L of water to the filter residue, and repeat the leaching once under the same conditions. The combined filtrates were centrifuged (3000r / min×20min), and the supernatant was concentrated to 1.5L under reduced pressure at 50-55°C to obtain a 60°C water-extraction concentrate. Add 15L of water to the medicinal material residue, decoct at 100°C for 1.5 hours, filter, centrifuge the filtrate, and concentrate the supernatant to 1.5L under reduced pressure at 50-55°C to obtain a water-extracted concentrate at 100°C.

[0079] Add 4 times the volume of ethanol to the 60°C water-extraction concentrate and the 100°C water-extracti...

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Abstract

The invention relates to a morinda root water extract, coarse polysaccharides and oligosaccharide and polysaccharide components and preparation methods and application thereof. The morinda root waterextract and the coarse polysaccharides are extracted from morinda roots by a mode of water extraction, then the coarse polysaccharides are separated to obtain the oligosaccharide and polysaccharide components in the coarse polysaccharides, and properties are measured. Experiments show that both the morinda root water extract and the coarse polysaccharides provided by the invention can promote theproliferation of spleen cells and the secretion of cell factors in mice, promote the proliferation of human liver cells, reduce the damage of toxic agents on cells, inhibit the expression of hepatitisB surface antigens and core antigens, inhibit the proliferation of liver cancer cells as well as inhibit the damage of ConA to the livers and kidneys of the mice, have obviously better biological activity than that of the morinda root oligosaccharides, polysaccharides or morinda root polysaccharide components prepared by other methods, and have the prospect of being developed into immunomodulators, anti-liver injury and anti-tumor drugs or health-care food. Preparation steps are simple, and preparation processes have less pollution to the environment and are suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical fields of traditional Chinese medicine, hepatology and immunology, and relates to Morinda officinalis oligosaccharides, crude polysaccharides and polysaccharide components, their preparation methods and uses. Specifically, the present invention also relates to a pharmaceutical composition and the use of the Morinda officinalis water extract, oligosaccharides and polysaccharides in the preparation of liver protection and immune regulation. Background technique [0002] Morinda officinalis is one of the four famous southern medicines in my country. It is derived from the dry root of Morinda officinalis How in the Rubiaceae family. It is distributed in tropical and subtropical regions in Guangdong, Fujian, Guangxi, Hainan and other provinces. Morinda officinalis has the effects of invigorating kidney yang, strengthening muscles and bones, and dispelling wind-dampness, and is known as "the key medicine for invigoratin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/746A61P1/16A61P35/00A61P37/02A23L33/105A23L33/125C07H3/02C07H1/08A61K125/00
CPCA23V2002/00A61K36/746A61K2236/331A61K2236/39A61K2236/51A61K2236/53A23L33/105A23L33/125A61P1/16A61P35/00A61P37/02C07H1/08C07H3/02C08B37/00C08B37/0003A23V2200/324A23V2200/30A23V2200/334A23V2200/308A23V2250/21A23V2250/51A23V2300/14
Inventor 高月求刘晓燕单俊杰李曼徐慧琳周振华陈彦旭高亚婷麻浩赵修南孙学华张鑫
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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