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A composite nanoprobe and its method for in vivo ratiometric imaging detection

A nanoprobe and living body technology, applied in the field of nanoprobes, can solve the problems of reducing the penetration depth and limitations of fluorescence imaging, and achieve the effects of overcoming uneven distribution, reducing interference, and improving imaging signal-to-noise ratio

Active Publication Date: 2021-07-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, most probes use visible light to do the ratio, and only a few probes realize the ratio of visible light to near-infrared light, which is greatly limited in the application of in vivo imaging.
In the process of fluorescence imaging, many substances in the organism, such as protein and hematoporphyrin, will also be excited, resulting in serious background fluorescence signals. Greatly reduced penetration depth for fluorescence imaging

Method used

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  • A composite nanoprobe and its method for in vivo ratiometric imaging detection
  • A composite nanoprobe and its method for in vivo ratiometric imaging detection
  • A composite nanoprobe and its method for in vivo ratiometric imaging detection

Examples

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Effect test

Embodiment 1

[0039] Example 1 Preparation of Responsive Composite Nanoprobes

[0040] (1) Preparation of mercury ion-responsive composite nanoprobes

[0041] In the composite nanoprobe in this embodiment, the organic fluorescent dye used is a rhodamine dye (FUC-1) that is responsive to mercury ions, and the inorganic rare earth nanomaterial used is NaYF with a core-shell structure. 4 :Nd@NaYF 4 , select amphiphilic polymer (F127) for self-assembly, and finally form a water-soluble composite nanoprobe responsive to mercury ions.

[0042] (2) Preparation of nitroreductase-responsive composite nanoprobes

[0043] In the composite nanoprobe in this example, the organic fluorescent dye used is a cyanine dye (Cy7-1) responsive to nitroreductase, and the inorganic rare earth nanomaterial used is NaYF 4 :Nd, choose silica for self-assembly, and finally form a water-soluble composite nanoprobe responsive to nitroreductase.

[0044] (3) Preparation of hypochlorous acid-responsive composite nanop...

Embodiment 2

[0047] Example 2 Photophysical Properties and Responsiveness of Composite Nanoprobes

[0048] (1) Mercury ion responsiveness research

[0049] The composite nanoprobe prepared in Example 1(1) was dispersed in an aqueous solution, and the ultraviolet absorption and fluorescence emission spectra were tested. Under 808nm excitation, the composite probe has a near-infrared emission peak at 1060nm, which can be attributed to the inorganic rare earth nanomaterial Nd 3+ feature emission. After adding mercury ions to the aqueous solution, the emission peak at 1060nm did not change, but a new near-infrared emission appeared at 730nm, and showed a trend of increasing gradually with the addition of mercury ions. This peak can be attributed to the frequency up-conversion emission generated by the reaction of the organic fluorescent dye FUC-1 with mercury ions.

[0050] (2) Study on the responsiveness of nitroreductase

[0051] The composite nanoprobe prepared in Example 1(2) was dispe...

Embodiment 3

[0055] Example 3 Composite nanoprobes for ratiometric imaging in vivo

[0056] (1) In vivo mercury ion ratio imaging

[0057] The composite nanoprobe prepared in Example 1(1) was dispersed in phosphate buffer solution, injected into mercury poisoning model mice and control mice 0.2 mL through the tail vein, and optically imaged on an in vivo imager after anesthesia. Using 808nm laser as the excitation light source, when the emitted light with a wavelength of 730±20nm is collected, the signal in the liver of the mercury poisoning model mice is significantly stronger than that of the control group mice, and the signal is attributed to the organic fluorescent dye FUC-1 and mercury ions The emission peak generated after the action verified the responsiveness of the composite nanoprobe to excess mercury ions in the liver of mercury poisoning model mice. When collecting the emission signal with a wavelength of 1060±20nm, the intensity of the two groups is equivalent, and the signal...

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Abstract

The invention belongs to the technical field of nanoprobes, and discloses a composite nanoprobe and a method for ratio imaging detection of a living body. The composite nanoprobe includes near-infrared organic fluorescent dyes and near-infrared inorganic rare earth nanomaterials, wherein the near-infrared organic fluorescent dyes contain cyanine, rhodamine or BODIPY probe molecules, and the near-infrared inorganic rare earth nanomaterials contain core structure or core‑shell structure of rare earth fluorides. Under the excitation of near-infrared light, both organic fluorescent dyes and rare earth nanomaterials can emit near-infrared light without interfering with each other. When interacting with specific species, the emission peak position or intensity of organic fluorescent dyes will change, while the emission peaks of rare earth nanomaterials will not be affected, resulting in the ratio of the two near-infrared emission peaks changing with the addition of specific species. Regular changes occur, and the detection and imaging functions of specific species can be realized by using this ratio change as a detection signal.

Description

technical field [0001] The invention belongs to the technical field of nanoprobes, in particular to a composite nanoprobe and a method for ratio imaging detection of a living body. Background technique [0002] Fluorescent probes have high sensitivity and can provide instantaneous and spatial information of target biomolecules, and are widely used in the fields of life science and basic medicine. Fluorescent probes have played an important role in people's understanding of life, helping people explore unknown and interesting mysteries of life. However, the vast majority of fluorescent probes are fluorescence-enhanced probes and do not have specific targeting. The design of such fluorescent probes can no longer meet the needs of people to observe certain physiological processes. For example, in organisms, there will be uneven distribution and high and low content of the substances to be detected. At this time, simple fluorescence-enhanced probes cannot solve the problems of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/84C09K11/06C09K11/85
CPCC09K11/06C09K11/7773C09K2211/1007C09K2211/1029C09K2211/1088G01N21/6486G01N21/84
Inventor 李富友韩春苗冯玮
Owner FUDAN UNIV
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