Unlock instant, AI-driven research and patent intelligence for your innovation.

Mercury ion detection method

A mercury ion, quantitative detection technology, applied in the field of mercury ion detection, can solve the problems of complex synthesis, insufficient selectivity, and insufficient response speed, and achieves the effects of simple synthesis, good stability, and favorable promotion and application.

Inactive Publication Date: 2018-11-09
广煜医药科技(宁夏)有限公司
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems with the currently reported colorimetric and fluorescent probes, including insufficient selectivity, insufficient response speed, and complex synthesis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mercury ion detection method
  • Mercury ion detection method
  • Mercury ion detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]

[0034] (Scheme 1) The first step is to dissolve resorcinol and 4-ethylamino ketoacid in trifluoroacetic acid solution and heat to reflux for 24 hours, then add ethyl acetate to reflux for 15 minutes after rotary evaporation, and obtain a red solid by suction filtration. Dissolve 387mg (1mmol) of the product of the first step and 430.16mg (4mmol) of dimethylaminothioformyl chloride in dichloromethane, add DIPEA and stir at room temperature for 20h. Use the dichloromethane system for column chromatography to obtain 370mg of an orange solid , and the yield was 74%.

[0035] (Scheme 2) The first step is to dissolve resorcinol and 4-ethylamino ketoacid in trifluoroacetic acid solution and heat to reflux for 24 hours. After rotary evaporation, ethyl acetate is added to reflux for 15 minutes, and a red solid is obtained by suction filtration. Dissolve 387mg (1mmol) of the product of the first step and 645.24mg (6mmol) of dimethylaminothioformyl chloride in dichloromethane...

Embodiment 2

[0041] figure 1 is the probe (5μM) added with Hg 2+ (5μM) and H 2 o 2 (0,10,20,40,60,80,100mM) fluorescence spectrum. The above determinations were carried out in 5mM HEPES, pH 7.4 aqueous solution, the probe used was the probe prepared in Example 1, and all spectral tests were measured at 25°C. From the illustration, we can see that the fluorescence changes significantly after adding different concentrations of hydrogen peroxide in the presence of mercury ions. It shows that the probe has a good response to mercury ions under the catalysis of 60mM hydrogen peroxide.

Embodiment 3

[0043] figure 2 is the probe (5μM) added with Hg 2+ (5μM) and H 2 o 2 (60mM) fluorescence spectrum.

[0044] From the illustration, we can see that the fluorescence signals of only adding mercury ions or hydrogen peroxide are basically unchanged, and only under the catalytic conditions of hydrogen peroxide, mercury ions have a very obvious response to the probe. It shows that the probe can detect mercury ions sensitively.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a highly selective and ultra-sensitive mercury ion detection method. Specifically, a probe used by the method of the present invention is fluorescein compounds, which can be used as mercury ion colorimetric fluorescence probes for mercury ion detection. The probes can achieve at least one of the following technical effects: highly selective recognition of mercury ions; rapid recognition of mercury ions; ultra-sensitive analysis of mercury ions; stable properties, and long-term preservation and use; and strong anti-interference ability.

Description

technical field [0001] The invention relates to a method for detecting mercury ions using fluorescein compounds as fluorescent probes for mercury ions. The method can quickly identify mercury ions with high selectivity and sensitivity, or it can measure the concentration of mercury ions in samples. Background technique [0002] Mercury is a metal element with severe physiological toxicity. Due to its persistence, easy migration and high bioaccumulation, it has become one of the most concerned environmental pollutants. Inorganic mercury ions in the environment can be converted by organisms into highly toxic methylmercury under certain conditions. Inorganic mercury mainly affects the kidneys, while methylmercury mainly damages the nervous system after entering the human body, especially the central nervous system. Both can be highly enriched in biological tissues through the food chain, thus causing great harm to humans and nature. Mercury poisoning will have an extremely ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/06G01N21/64G01N21/33G01N21/78
CPCC09K11/06C09K2211/1088G01N21/33G01N21/643G01N21/78
Inventor 不公告发明人
Owner 广煜医药科技(宁夏)有限公司