A kind of composite microbial culture and its application in the production of carotenoids

A technology of compounding microorganisms and carotene, applied in the field of microorganisms, to achieve the effect of low raw materials and reducing enterprise costs

Active Publication Date: 2020-08-07
黑龙江省黑沃土生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the defects in the production of carotenoids in the prior art, and provide a compound microbial culture and its application in the production of carotenoids

Method used

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  • A kind of composite microbial culture and its application in the production of carotenoids
  • A kind of composite microbial culture and its application in the production of carotenoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A composite microbial culture, which includes Bacillus subtilis, Rhodopseudomonas capsulata and Rhodotorula glutinis;

[0035] First crush the rice straw to 2cm, and then put it under the conditions of 1.5MPa pressure and 10min residence time for steam explosion pretreatment;

[0036] Pass the steam-blasted rice straw through a 50-mesh sieve, collect the under-sieve, and mix it with distiller’s grains in a mass ratio of 3:1 to obtain a mixture, then add it to water that accounts for 2 times the weight of the mixture, and heat it to 60°C. Stir at 100 rpm for 90 min, then heat to 121°C, keep for 5 min, and cool to room temperature naturally to obtain a culture solution;

[0037] The Bacillus subtilis seed solution was cultured in a photosynthetic culture fermenter containing the culture solution at an inoculum of 7% (volume ratio). The temperature was 30°C, the dissolved oxygen concentration was 1 mg / L, the light intensity was 2000 lux, and the culture time For 36h, then inocul...

Embodiment 2

[0042] A composite microbial culture, which includes Bacillus subtilis, Rhodopseudomonas capsulata and Rhodotorula glutinis;

[0043] First crush the rice straw to 5cm, and then put it under the conditions of pressure 1.8MPa and residence time of 5min for steam explosion pretreatment;

[0044] Pass the steam-blasted rice straw through a 50-mesh sieve, collect the under-sieve, and then mix it with distiller’s grains in a mass ratio of 3:1 to obtain a mixture, then add it to water that accounts for 3 times the weight of the mixture, and heat to 70°C. Stir at 100 rpm for 80 minutes, then heat to 121°C, keep for 4 minutes, and naturally cool to room temperature to obtain a culture solution;

[0045] The Bacillus subtilis seed solution was cultured in a photosynthetic culture fermenter containing the culture solution at an inoculum of 6% (volume ratio), the temperature was 30℃, the dissolved oxygen concentration was 2mg / L, the light intensity was 3000lux, and the culture time For 24h, th...

Embodiment 3

[0050] The changes of main components of rice straw by blasting are shown in Table 1:

[0051] Table 1

[0052] index Before blasting After blasting Hemicellulose and cellulose%53.740.5 Lignin%27.922.1 Xylooligosaccharides%03.4

[0053] Conclusion: Blasting damages the cell wall of the straw, and part of the hemicellulose and cellulose are degraded and dissolved out, which is conducive to the enzymatic hydrolysis of cellulose by cellulase; the cellulose crystallinity and degree of polymerization decrease during the pre-blasting process, and the hemicellulose passes through Autohydrolysis degrades into monosaccharides and oligosaccharides, which can be used as a carbon source for strains.

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Abstract

The invention belongs to the technical field of microorganisms and discloses a compound microorganism culture for preparing carotenoid. The compound microorganism culture comprises bacillus subtilis,rhodopseudomonas capsulate and rhodotorula glutinis. The compound microorganism culture increases yield of carotenoid and lowers culture cost.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a composite microorganism culture and its application in the production of carotenoids. Background technique [0002] Carotenoids are a type of fat-soluble pigments, mostly insoluble in water, soluble in organic solvents, yellow, orange or red polyene compounds. Because of their wide distribution, diverse structures and more functions, they have become important Natural pigments. It exists in plants, animals and microorganisms at the same time. Carotenoids have a variety of biological effects due to their structural properties. Studies have shown that carotenoids may have the effect of preventing and treating heart disease, cancer, cataracts and several chronic human diseases and other biological activities. In addition, as an excellent food additive and nutritional supplement to improve human nutrition, carotenoids have long been recognized by countries all over t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12P23/00C12R1/125C12R1/38C12R1/645
CPCC12N1/16C12N1/20C12P23/00
Inventor 李玉刚钟银珊应建航应佳丽
Owner 黑龙江省黑沃土生物科技有限公司
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