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Method for producing biological butanol and bioethanol by using corn straw as main raw material

A technology of corn stalks, the main raw material, applied in the field of fermentation, which can solve the problems of difficulty in straw utilization, low efficiency, and high cost of bio-fermentation alcohol production, and achieve the effects of good wall breaking effect, cost reduction, and improvement of fermentation efficiency

Active Publication Date: 2018-09-28
河北易高生物燃料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the defects of difficulty in straw utilization, high cost and low efficiency of bio-fermentation alcohol production in the prior art, and to provide a method for producing bio-butanol and bio-ethanol by using corn straw as the main raw material for fermentation

Method used

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  • Method for producing biological butanol and bioethanol by using corn straw as main raw material
  • Method for producing biological butanol and bioethanol by using corn straw as main raw material
  • Method for producing biological butanol and bioethanol by using corn straw as main raw material

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The method for producing bio-butanol and bio-ethanol by using corn stalks as the main raw material for fermentation comprises the following steps:

[0038] First crush the corn stalks to less than 5cm, then put them under the conditions of pressure 1.5MPa and residence time 15min for steam explosion pretreatment, and then carry out blasting; pass the steam explosion treated corn stalks through a 50-mesh sieve, collect the sieve, Then add the undersize to 2 times the weight of water, heat to 60°C, stir at 100rpm for 90min under heat preservation conditions, then heat to 121°C, keep heat for 10min, and naturally cool to room temperature to obtain a culture solution;

[0039] Trichoderma konii seed liquid (density 1 × 108 cfu / mL) and Aspergillus niger seed solution (density 1×10 8 cfu / mL) mixed according to the volume ratio of 2:1, and then inoculated into the culture medium according to the inoculum amount of 6-8%, and cultivated for 96 hours. During the cultivation, the ...

Embodiment 2

[0042] The method for producing bio-butanol and bio-ethanol by using corn stalks as the main raw material for fermentation comprises the following steps:

[0043] Firstly crush the corn stalks to less than 5cm, then place them under the conditions of pressure 2MPa and residence time 10min for steam explosion pretreatment, and then carry out blasting; pass the steam explosion treated corn stalks through a 50-mesh sieve, collect the sieved material, and then Add the undersieve to 1.5 times the weight of water, heat to 60°C, stir at 100rpm for 90min under heat preservation conditions, then heat to 121°C, keep heat for 10min, and naturally cool to room temperature to obtain a culture solution;

[0044] Trichoderma konii seed liquid (density 1 × 10 8 cfu / mL) and Aspergillus niger seed solution (density 1×10 8 cfu / mL) mixed according to the volume ratio of 3:2, and then inoculated into the culture medium according to the inoculum amount of 6-8%, and cultivated for 96 hours. During ...

Embodiment 3

[0047] The changes in the main components of corn stalks caused by blasting are shown in Table 1:

[0048] Table 1

[0049] index

[0050] Conclusion: Blasting caused the cell wall of straw to be destroyed, and some hemicellulose and cellulose were degraded and dissolved, which was beneficial to the subsequent enzymatic hydrolysis of cellulose by cellulase; the crystallinity and polymerization degree of cellulose decreased during the blasting pretreatment process, and hemicellulose passed It is degraded into xylose by hydrolysis and can be used as a carbon source for the strain.

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PUM

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Abstract

The invention belongs to the technical field of fermentation, and discloses a method for producing biological butanol and bioethanol by using a corn straw as a main raw material, which comprises the following steps of: step (1) processing the straws, step (2) preparing a fermentation medium, and step (3) generating an alcohol. The method for producing biological butanol and bioethanol by using thecorn straw as a main raw material has the advantages of low raw material cost and high alcohol production efficiency.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for fermenting and producing bio-butanol and bio-ethanol by using corn stalks as main raw materials. Background technique [0002] Butanol is a colorless liquid with a smell of alcohol. It is miscible with ethanol, ether and many other organic solvents. Its vapor and air form explosive mixtures. The explosion limit is 1.45-11.25 (volume). It is mainly used to make n-butyl ester plasticizers of phthalic acid, aliphatic dibasic acid and phosphoric acid. They are widely used in various plastic and rubber products. They are also used in the production of butyraldehyde, butyric acid and butylamine in organic synthesis. And raw materials such as butyl lactate. Biobutanol is a biofuel similar to bioethanol. Its raw material and production process are similar to those of bioethanol, but biobutanol has low vapor pressure, has a large tolerance to impurity water...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/10C12P7/16C12R1/685C12R1/885C12R1/145C12R1/645
CPCC12P7/10C12P7/16C12P39/00C12P2201/00C12P2203/00Y02E50/10
Inventor 冯国青应鹏欢方彦钟银珊应建航
Owner 河北易高生物燃料有限公司
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