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A process for preparing and separating bio-butanol and bio-ethanol

A bio-ethanol and bio-butanol technology, applied in the field of fermentation, can solve environmental pollution and other problems, achieve good wall breaking effect, increase porosity and internal surface area, and increase industrial added value

Active Publication Date: 2022-04-19
黑龙江鸿展生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Corn stalks belong to agricultural waste, and most of them are discarded or burned at will. However, incineration will cause environmental pollution and has been banned by the state. At present, many companies are studying the utilization methods of corn stalks

Method used

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  • A process for preparing and separating bio-butanol and bio-ethanol
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  • A process for preparing and separating bio-butanol and bio-ethanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A process for preparing and separating bio-butanol and bio-ethanol, comprising the steps of:

[0042] First crush the corn stalks to less than 5cm, then put them under the conditions of pressure 1.5MPa and residence time 15min for steam explosion pretreatment, and then carry out blasting; pass the steam explosion treated corn stalks through a 50-mesh sieve, collect the sieve, Then add the undersize to 2 times the weight of water, heat to 60°C, stir at 100rpm for 90min under heat preservation conditions, then heat to 121°C, keep heat for 10min, and naturally cool to room temperature to obtain a culture solution;

[0043] Trichoderma konii seed liquid (density 1 × 10 8 cfu / mL) and Aspergillus niger seed solution (density 1×10 8 cfu / mL) mixed according to the volume ratio of 2:1, and then inoculated into the culture medium according to the inoculum amount of 6-8%, and cultivated for 96 hours. During the cultivation, the stirring speed was controlled at 100rpm, and the cul...

Embodiment 2

[0046] The method for producing bio-butanol and bio-ethanol by using corn stalks as the main raw material for fermentation comprises the following steps:

[0047] Firstly crush the corn stalks to less than 5cm, then place them under the conditions of pressure 2MPa and residence time 10min for steam explosion pretreatment, and then carry out blasting; pass the steam explosion treated corn stalks through a 50-mesh sieve, collect the sieved material, and then Add the undersieve to 1.5 times the weight of water, heat to 60°C, stir at 100rpm for 90min under heat preservation conditions, then heat to 121°C, keep heat for 10min, and naturally cool to room temperature to obtain a culture solution;

[0048] Trichoderma konii seed liquid (density 1 × 10 8 cfu / mL) and Aspergillus niger seed solution (density 1×10 8 cfu / mL) mixed according to the volume ratio of 3:2, and then inoculated into the culture medium according to the inoculum amount of 6-8%, and cultivated for 96 hours. During ...

Embodiment 3

[0051] The changes in the main components of corn stalks caused by blasting are shown in Table 1:

[0052] Table 1

[0053] index Before blasting after blasting Hemicellulose and Cellulose% 61.2 48.9 Lignin% 22.7 15.6 Xylooligosaccharide% 0 6.9 Cellooligosaccharides 0 3.1

[0054] Conclusion: Blasting caused the cell wall of straw to be destroyed, and some hemicellulose and cellulose were degraded and dissolved, which was beneficial to the subsequent enzymatic hydrolysis of cellulose by cellulase; the crystallinity and polymerization degree of cellulose decreased during the blasting pretreatment process, and hemicellulose passed It is degraded into xylose by hydrolysis and can be used as a carbon source for the strain.

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Abstract

The invention belongs to the field of fermentation technology, and discloses a process for preparing and separating bio-butanol and bio-ethanol, which includes the following steps: step 1) processing straw, step 2) preparing fermentation medium, step 3) producing alcohol, and step 4) Separation of alcohol and preparation of protein powder. The process of the invention can simultaneously obtain bio-butanol, bio-ethanol and bacterial protein powder, thereby increasing the added value.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a process for preparing and separating bio-butanol and bio-ethanol. Background technique [0002] Butanol is a colorless liquid with a smell of alcohol. It is miscible with ethanol, ether and many other organic solvents. Its vapor and air form explosive mixtures. The explosion limit is 1.45-11.25 (volume). It is mainly used to make n-butyl ester plasticizers of phthalic acid, aliphatic dibasic acid and phosphoric acid. They are widely used in various plastic and rubber products. They are also used in the production of butyraldehyde, butyric acid and butylamine in organic synthesis. And raw materials such as butyl lactate. Biobutanol is a biofuel similar to bioethanol. Its raw material and production process are similar to those of bioethanol, but biobutanol has low vapor pressure, has a large tolerance to impurity water when mixed with gasoline, and is less cor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/10C12P7/16C12N1/20C12N1/14C12R1/885C12R1/685C12R1/145C12R1/645
CPCC12N1/14C12N1/20C12P7/10C12P7/16C12P39/00C12P2203/00C12P2201/00Y02E50/10
Inventor 冯国青应鹏欢方彦钟银珊应建航
Owner 黑龙江鸿展生物科技股份有限公司
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