Method for preparing sodium glutamate through concentrating continuous freezing isoelectric point crystallization
A glutamic acid and isoelectric point technology, applied in the field of amino acid fermentation, can solve the problems of high concentration, low fermentation efficiency of glutamic acid process, large amount of waste water, etc., achieve low consumption, increase industrial added value, and reduce operating costs Effect
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Embodiment 1
[0027] A method for preparing glutamic acid by concentrated continuous freezing isoelectric point crystallization, comprising the steps of:
[0028] 1) Preparation of Corynebacterium glutamicum seed solution:
[0029] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13032 is inserted in Corynebacterium glutamicum seed culture medium, inoculum size is 3%, at 30 ℃, 150rpm shaking table is cultivated 18h, the Corynebacterium glutamicum seed liquid that obtains; The components of the Corynebacterium acidic acid seed culture medium are (the following are mass percentages): glucose 2.5%, corn steep liquor 3%, K 2 HPO 4 0.2%, Na 2 HPO 4 0.2%, biotin 0.5%, MgSO 4 0.2%, 0.025% urea, sterilized at 115°C for 15 minutes;
[0030] 2) Preparation of Brevibacterium lactose fermentum seed solution:
[0031] Brevibacterium lactofermentum (Brevibacterium lactofermentum) ATCC13869 was inoculated into the seed medium of Brevibacterium lactofermentum, the inoculation amount was 5%...
Embodiment 2
[0049] 1. Strain fermentation comparison test (same fermentation conditions): Corynebacterium glutamicum ATCC13032 was used as the fermentation strain, and the maximum glutamic acid production can reach 200g / L (see the applicant's previous patent cn201210211393 ); and adopt the mixed bacterial strain of the present invention to ferment, the output of glutamic acid reaches 218g / L, has improved 9%, is better than single bacterial strain fermentation greatly.
[0050] 2. Examples of wastewater treatment effects
[0051] Take Fufeng production workshop, extract waste liquid from glutamic acid, process waste liquid according to the method of embodiment 1, take samples to measure COD, ammonia nitrogen, total nitrogen data;
[0052] Compared with Example 1, the control 1 group did not add liquid bacterial agent; compared with Example 1, the control 2 group had only wheat bran as the adsorbent carrier. Sampling and determination of COD and ammonia nitrogen data, the test data are sh...
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