Method for preparing sodium glutamate through concentrating continuous freezing isoelectric point crystallization

A glutamic acid and isoelectric point technology, applied in the field of amino acid fermentation, can solve the problems of high concentration, low fermentation efficiency of glutamic acid process, large amount of waste water, etc., achieve low consumption, increase industrial added value, and reduce operating costs Effect

Inactive Publication Date: 2015-11-25
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this process is that the extraction yield is high, and the general total yield can reach 94%, but this process produces an additional 40% of high-concentration wastewater, and consumes an additional 100% of sulfuric acid and 45% of liquid ammonia
[0004] The defects in the glutamic acid process are mainly the low fermentation efficiency, the large amount of wastewater produced, and the high concentration. Overcoming the above defects is the direction of our research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing glutamic acid by concentrated continuous freezing isoelectric point crystallization, comprising the steps of:

[0028] 1) Preparation of Corynebacterium glutamicum seed solution:

[0029] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13032 is inserted in Corynebacterium glutamicum seed culture medium, inoculum size is 3%, at 30 ℃, 150rpm shaking table is cultivated 18h, the Corynebacterium glutamicum seed liquid that obtains; The components of the Corynebacterium acidic acid seed culture medium are (the following are mass percentages): glucose 2.5%, corn steep liquor 3%, K 2 HPO 4 0.2%, Na 2 HPO 4 0.2%, biotin 0.5%, MgSO 4 0.2%, 0.025% urea, sterilized at 115°C for 15 minutes;

[0030] 2) Preparation of Brevibacterium lactose fermentum seed solution:

[0031] Brevibacterium lactofermentum (Brevibacterium lactofermentum) ATCC13869 was inoculated into the seed medium of Brevibacterium lactofermentum, the inoculation amount was 5%...

Embodiment 2

[0049] 1. Strain fermentation comparison test (same fermentation conditions): Corynebacterium glutamicum ATCC13032 was used as the fermentation strain, and the maximum glutamic acid production can reach 200g / L (see the applicant's previous patent cn201210211393 ); and adopt the mixed bacterial strain of the present invention to ferment, the output of glutamic acid reaches 218g / L, has improved 9%, is better than single bacterial strain fermentation greatly.

[0050] 2. Examples of wastewater treatment effects

[0051] Take Fufeng production workshop, extract waste liquid from glutamic acid, process waste liquid according to the method of embodiment 1, take samples to measure COD, ammonia nitrogen, total nitrogen data;

[0052] Compared with Example 1, the control 1 group did not add liquid bacterial agent; compared with Example 1, the control 2 group had only wheat bran as the adsorbent carrier. Sampling and determination of COD and ammonia nitrogen data, the test data are sh...

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PUM

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Abstract

The invention belongs to the technical field of amino acid fermentation, and discloses a method for preparing sodium glutamate through concentrating continuous freezing isoelectric point crystallization. The method comprises the following steps: 1) preparing a corynebacterium glutamicum seed solution; 2) preparing a brevibacterium lactofermentum seed solution; 3) mixing and stirring; 4) preparing sodium glutamate through fermentation; 5) implementing concentrating continuous freezing isoelectric point crystallization; and 6) processing waste liquid. The method disclosed by the invention is high in fermentation efficiency, low in industrial energy consumption and is energy-saving and emission-reducing.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a method for preparing glutamic acid by concentration and continuous freezing isoelectric point crystallization. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. MSG is prepared from glutamic acid. [0003] The existing technologies for extracting glutamic acid from glutamic acid fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C12R1/13
Inventor 孙钦波程士清尤学波许传高
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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