A method of producing carotenoids

A carotene and compound microorganism technology, which is applied in the field of carotenoid production to achieve the effects of reducing enterprise costs, low raw materials and reducing usage

Active Publication Date: 2021-01-22
杭州园泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the defects such as the production of carotenoids in the prior art and provide a method for producing carotenoids

Method used

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  • A method of producing carotenoids
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Embodiment 1

[0039] A method for producing carotenoids, comprising the steps of:

[0040] Centrifuge the composite microbial culture at 8000rpm and 4°C for 20min, discard the supernatant, collect the bacteria, then add acetone according to the amount of 1kg bacteria: 1L acetone, and then ultrasonically break the wall, the ultrasonic power is 300W, and the time for each ultrasonic The time is 10s, the interval time is 10s, the total ultrasonic time is 30min, then stand still for 30min, then centrifuge at 5000rpm for 5min, collect the supernatant and precipitate, add the same volume of acetone to the precipitate, use ultrasonic treatment for 15min, ultrasonic power is 300W, Each ultrasonic time is 10s, the interval time is 10s, then centrifuged at 5000rpm for 3min, and the supernatant is collected; the two supernatants are combined, and then evaporated to dryness with a rotary evaporator at low temperature, the residue is dissolved with twice the weight of ether, and then added A 30% KOH met...

Embodiment 2

[0049] A method for producing carotenoids, comprising the steps of:

[0050] Centrifuge the complex microbial culture at 8000rpm and 4°C for 20min, discard the supernatant, collect the bacteria, then add acetone according to the amount of 1kg bacteria: 1.5L acetone, and then ultrasonically break the wall, the ultrasonic power is 300W, each ultrasonic The time is 10s, the interval time is 10s, the total ultrasonic time is 30min, then stand still for 30min, then centrifuge at 5000rpm for 5min, collect the supernatant and precipitate, add the same volume of acetone to the precipitate, use ultrasonic treatment for 15min, and the ultrasonic power is 300W , each ultrasonic time is 10s, the interval time is 10s, then centrifuged at 5000rpm for 3min, and the supernatant is collected; the two supernatants are combined, and then evaporated to dryness with a rotary evaporator at low temperature, the residue is dissolved in ether, and then add 6 / 6 A volume of KOH methanol solution with a ...

Embodiment 3

[0059] The changes in the main components of rice straw caused by blasting are shown in Table 1:

[0060] Table 1

[0061] index Before blasting after blasting Hemicellulose and Cellulose% 53.7 40.5 Lignin % 27.9 22.1 Xylooligosaccharide% 0 3.4

[0062] Conclusion: Blasting caused the cell wall of straw to be destroyed, and some hemicellulose and cellulose were degraded and dissolved, which was beneficial to the subsequent enzymatic hydrolysis of cellulose by cellulase; the crystallinity and polymerization degree of cellulose decreased during the blasting pretreatment process, and hemicellulose passed It is degraded into monosaccharides and oligosaccharides by hydrolysis, which can be used as a carbon source for the strain.

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Abstract

The invention belongs to the technical field of biology and discloses a method for producing carotenoid. The method comprises the following steps: centrifuging a compound microorganism culture; removing supernatant and collecting thalli; then adding acetone and carrying out ultrasonic wall breaking; then standing for 30min; then centrifuging and collecting supernatant and sediment; adding acetonewith the volume the same as that of the sediment into the sediment; carrying out ultrasonic treatment and collecting supernatant; combining the supernatant of the two times; then evaporating and drying at low temperature by utilizing a rotary evaporator; dissolving residues with ethyl ether; then adding a KOH methanol solution and standing for 10 to 30min; then adding a NaCl solution and layeringa solvent; collecting an upper-layer ethyl ether phase and evaporating and drying the ethyl ether phase to obtain a carotenoid product. According to the method disclosed by the invention, the purity of a prepared carotenoid product is relatively high and reaches 94 percent or above, and is converted to the yield which reaches 90 percent or above.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing carotenoids. Background technique [0002] Carotenoids are a kind of fat-soluble pigments, most of which are difficult to dissolve in water but soluble in organic solvents. They are yellow, orange or red polyene compounds. Because of their wide distribution, diverse structures and many functions, they have become an important of natural pigments. It exists simultaneously in plants, animals and microorganisms. Carotenoids have a variety of biological effects due to their structural properties, and studies have shown that carotenoids may have preventive and other biologically active effects on heart disease, cancer, cataracts, and several chronic human diseases. In addition, carotenoids, as an excellent food additive and nutritional supplement for improving human nutrition, have long been recognized by countries all over the world for their excellen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P23/00C12N1/20C12N1/16C12R1/125C12R1/38C12R1/645
CPCC12N1/16C12N1/20C12P23/00C12P39/00
Inventor 李玉刚钟银珊应建航应佳丽
Owner 杭州园泰生物科技有限公司
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