Corn salt-tolerant gene and molecular marker and application thereof

A technology of molecular markers and salt-tolerant genes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of accelerating genetic improvement

Inactive Publication Date: 2018-11-13
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is a s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Corn salt-tolerant gene and molecular marker and application thereof
  • Corn salt-tolerant gene and molecular marker and application thereof
  • Corn salt-tolerant gene and molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1 Genetic analysis of corn salt tolerance and location and cloning of salt tolerance genes

[0030] 280 Beijing 724×D9H F 2 The material, the seeds of which were sterilized with 1% NaClO for 10 minutes, washed with sterilized water three times, and then hydroponically planted in a corn seedling identification device (disclosed in Chinese Patent ZL200920177285.0). The culture condition is controlled at 26±1℃, 12h light / 12h dark, light intensity is 150-180μmol m -2 s -1 , The humidity is 70%. After culturing in an aqueous solution containing 100mM NaCl for 10 days, root length was used as the salt-tolerant phenotype. It was found that among 280 materials, 215 materials showed salt tolerance (root length ≥5cm), and 65 materials showed salt sensitivity (root length 2 1:3=0.48, P=0.49), it can be seen that the salt tolerance of corn root length is controlled by a dominant single gene.

[0031] Preliminary localization of salt tolerance genes using BSR-seq technology: Beij...

Example Embodiment

[0045] Example 2 Development and application of INDEL molecular markers related to corn salt tolerance traits

[0046] ZmST1 has a 4bp base insertion / deletion difference in the salt-tolerant corn inbred line Jing724 and the salt-sensitive inbred line D9H. The functional molecular marker was developed based on the sequence difference, and the functional site of the developed functional molecular marker ST35 is TCTT / -, the KASP primer information is shown in Table 1. After 50 parts of corn DH line materials were cultured in an aqueous solution containing 100 mM NaCl for 10 days, the root length was measured with a ruler, and the leaf DNA was extracted with TianGen's plant DNA extraction kit. Using this functional molecular marker, according to the laboratory of the Government Chemist (LGC) company’s standard experimental procedures, the KASP method was used to detect the InDel site, and it was found that the application of the functional molecular marker of this application to gen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a corn salt-tolerant gene and a molecular marker and application thereof. An F2 material with a salt-tolerant phenotype and an F2 material with a salt-sensitive phenotype are selected, and a BSR-seq technique is adopted for primary positioning of the corn salt-tolerant gene in a physical section of a genome first chromosome 167Mb-183Mb; then, 3635 F2 materials of Jing 724*D9H and a map-based cloning technique are adopted for fine positioning of the salt-tolerant gene in a section of 129kb (Chr1:180862086-180991157,B73 RefGen_v3); genes in the section are subjected to sequence analysis, functional annotation analysis and mutant allelic test to verify that GRMZM2G098494 is a salt-tolerant gene and named ZmST1, and the molecular marker of the gene is developed and can be used for early prediction and screening of corn salt tolerance characters and screening of salt-tolerant genetic resources.

Description

technical field [0001] The invention relates to the technical field of crop molecular marker assisted breeding, in particular to a corn salt-tolerant gene and its InDel molecular marker and application thereof. Background technique [0002] Corn is an important grain, feed and economic crop. However, it is a salt sensitive crop. More than 20% of the cultivated land in the world is salt-damaged land, which seriously restricts the growth, development and yield of maize (Munns et al., 2008, Annual Review of Plant Biology 59: 51-681). With the gradual reduction of arable land, the development and utilization of salt-tolerant maize germplasm resources is more urgent and necessary. Therefore, how to improve the salt tolerance of corn and cultivate new varieties of salt-tolerant corn is an urgent problem to be solved in the world, which is of great significance to the long-term development of the corn industry. [0003] Genetic factors are the root cause of the differences in sa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C07K14/415C12Q1/6895C12N15/11C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8273C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵衍鑫赵久然骆美洁宋伟张云霞
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products