Corn salt-tolerant gene and molecular marker and application thereof
A technology of molecular markers and salt-tolerant genes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of accelerating genetic improvement
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[0029] Example 1 Genetic analysis of corn salt tolerance and location and cloning of salt tolerance genes
[0030] 280 Beijing 724×D9H F 2 The material, the seeds of which were sterilized with 1% NaClO for 10 minutes, washed with sterilized water three times, and then hydroponically planted in a corn seedling identification device (disclosed in Chinese Patent ZL200920177285.0). The culture condition is controlled at 26±1℃, 12h light / 12h dark, light intensity is 150-180μmol m -2 s -1 , The humidity is 70%. After culturing in an aqueous solution containing 100mM NaCl for 10 days, root length was used as the salt-tolerant phenotype. It was found that among 280 materials, 215 materials showed salt tolerance (root length ≥5cm), and 65 materials showed salt sensitivity (root length 2 1:3=0.48, P=0.49), it can be seen that the salt tolerance of corn root length is controlled by a dominant single gene.
[0031] Preliminary localization of salt tolerance genes using BSR-seq technology: Beij...
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[0045] Example 2 Development and application of INDEL molecular markers related to corn salt tolerance traits
[0046] ZmST1 has a 4bp base insertion / deletion difference in the salt-tolerant corn inbred line Jing724 and the salt-sensitive inbred line D9H. The functional molecular marker was developed based on the sequence difference, and the functional site of the developed functional molecular marker ST35 is TCTT / -, the KASP primer information is shown in Table 1. After 50 parts of corn DH line materials were cultured in an aqueous solution containing 100 mM NaCl for 10 days, the root length was measured with a ruler, and the leaf DNA was extracted with TianGen's plant DNA extraction kit. Using this functional molecular marker, according to the laboratory of the Government Chemist (LGC) company’s standard experimental procedures, the KASP method was used to detect the InDel site, and it was found that the application of the functional molecular marker of this application to gen...
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