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Corn salt-tolerant gene and molecular marker and application thereof

A technology of molecular markers and salt-tolerant genes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of accelerating genetic improvement

Inactive Publication Date: 2018-11-13
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, it is a salt-sensitive crop

Method used

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  • Corn salt-tolerant gene and molecular marker and application thereof
  • Corn salt-tolerant gene and molecular marker and application thereof
  • Corn salt-tolerant gene and molecular marker and application thereof

Examples

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Effect test

Embodiment 1

[0029] Example 1 Genetic Analysis of Maize Salt Tolerance and Mapping and Cloning of Salt Tolerance Genes

[0030] 280 Kyo 724×D9H F 2 For materials, the seeds were sterilized with 1% NaClO for 10 minutes, washed three times with sterilized water, and then planted hydroponically in a corn seedling identification device (disclosed in Chinese patent ZL200920177285.0). The culture conditions are controlled at 26±1°C, 12h light / 12h dark, and the light intensity is 150-180μmol m -2 the s -1 , with a humidity of 70%. After cultivating in an aqueous solution containing 100mMNaCl for 10 days, the root length was used as the salt-tolerant phenotype, and among the 280 materials, 215 materials showed salt tolerance (root length ≥ 5cm), and 65 materials showed salt sensitivity (root length ≥ 5 cm). 2 1:3=0.48, P=0.49), it can be seen that the salt tolerance of maize root length is controlled by a dominant single gene.

[0031] Preliminary mapping of salt tolerance genes using BSR-seq ...

Embodiment 2

[0045] Example 2 Development and Application of INDEL Molecular Markers Related to Maize Salt Tolerance

[0046] ZmST1 has a 4 bp base insertion / deletion difference between the salt-tolerant maize inbred line Jing 724 and the salt-sensitive inbred line D9H, and functional molecular markers were developed based on the sequence differences. The functional molecular marker ST35 functional site developed is TCTT / -, its KASP primer information is shown in Table 1. After culturing 50 parts of corn DH series materials in an aqueous solution containing 100 mM NaCl for 10 days, the root length was measured with a ruler, and leaf DNA was extracted with a plant DNA extraction kit from TianGen Company. Using this functional molecular marker, according to the standard experimental procedures of Laboratory of the Government Chemist (LGC) company, the KASP method was used to detect the InDel site, and it was found that the salt tolerance obtained by applying the functional molecular marker ...

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Abstract

The invention provides a corn salt-tolerant gene and a molecular marker and application thereof. An F2 material with a salt-tolerant phenotype and an F2 material with a salt-sensitive phenotype are selected, and a BSR-seq technique is adopted for primary positioning of the corn salt-tolerant gene in a physical section of a genome first chromosome 167Mb-183Mb; then, 3635 F2 materials of Jing 724*D9H and a map-based cloning technique are adopted for fine positioning of the salt-tolerant gene in a section of 129kb (Chr1:180862086-180991157,B73 RefGen_v3); genes in the section are subjected to sequence analysis, functional annotation analysis and mutant allelic test to verify that GRMZM2G098494 is a salt-tolerant gene and named ZmST1, and the molecular marker of the gene is developed and can be used for early prediction and screening of corn salt tolerance characters and screening of salt-tolerant genetic resources.

Description

technical field [0001] The invention relates to the technical field of crop molecular marker assisted breeding, in particular to a corn salt-tolerant gene and its InDel molecular marker and application thereof. Background technique [0002] Corn is an important grain, feed and economic crop. However, it is a salt sensitive crop. More than 20% of the cultivated land in the world is salt-damaged land, which seriously restricts the growth, development and yield of maize (Munns et al., 2008, Annual Review of Plant Biology 59: 51-681). With the gradual reduction of arable land, the development and utilization of salt-tolerant maize germplasm resources is more urgent and necessary. Therefore, how to improve the salt tolerance of corn and cultivate new varieties of salt-tolerant corn is an urgent problem to be solved in the world, which is of great significance to the long-term development of the corn industry. [0003] Genetic factors are the root cause of the differences in sa...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12Q1/6895C12N15/11C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8273C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵衍鑫赵久然骆美洁宋伟张云霞
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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