Unlock instant, AI-driven research and patent intelligence for your innovation.
Probe for identifying bacteria, identification method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for bacteria and probes, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Simple, low professional requirements, low price effect
Active Publication Date: 2020-08-04
SHANGHAI INST OF MEASUREMENT & TESTING TECH
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
CN106086173A discloses a rapid bacterial detection method based on upconversion fluorescenceresonanceenergy transfer, in which the nucleic acidaptamer that can specifically recognize target bacteria is modified on the surface of gold nanoparticles, and the surface modification of rare earth upconversion fluorescent particles is complementary to the aptamer cDNA, resulting in fluorescence quenching, high sensitivity and good specificity, but the operation process requires professional operation, the detection of a single strain, narrow scope of application
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0073] Thirty-two fluorophore-labeled DNA probes were assembled into a kit with a single-layer graphene oxide nanosheet solution at a concentration of 1 mg / mL, phosphate buffer, TE buffer solution, and conventional reagents;
[0074] The sequence of the DNA probe is as follows:
[0075] SEQ ID No.1 is CCCCCCCCCCCCCCC, named P0;
[0076] SEQ ID No.2 is CCCCCCCCCCCCTTT, named as P1;
[0077] SEQ ID No.3 is CCCCCCCCCTTTCCC, named P2;
[0078] SEQ ID No.4 is CCCCCCCCCTTTTTT, named as P3;
[0079] SEQ ID No.5 is CCCCCCTTTTCCCCCC, named P4;
[0080] SEQ ID No.6 is CCCCCCTTTTCCCTTT, named as P5;
[0081] SEQ ID No.7 is CCCCCCTTTTTTCCC, named P6;
[0082] SEQ ID No.8 is CCCCCCTTTTTTTTT, named as P7;
[0083] SEQ ID No.9 is CCCTTTTCCCCCCCCC, named P8;
[0084] SEQ ID No.10 is CCCTTTCCCCCCTTT, named as P9;
[0085] SEQ ID No.11 is CCCTTTCCCTTTCCC, named as P10;
[0086] SEQ ID No.12 is CCCTTTCCCTTTTTTT, named as P11;
[0087] SEQ ID N...
Embodiment 2
[0108] 1. Randomly select eight fluorophore-labeled DNA probes from 32 probes. The fluorophore-labeled position is at the 5' end. The sequence is as follows:
[0109] The SEQ ID No.1: FAM-CCCCCCCCCCCCCCCC is named as P0.
[0110] Said SEQ ID No.5: FAM-CCCCCCTTTTCCCCCC is named as P4.
[0111] The SEQ ID No.11: FAM-CCCTTTCCCTTTTCCC is named as P10.
[0112] Said SEQ ID No.15: FAM-CCCTTTTTTTTTTCCC is named as P14.
[0113] The SEQ ID No.18: FAM-TTTCCCCCCCCCTTTT is named as P17.
[0114] Said SEQ ID No.22: FAM-TTTCCCTTTTCCCTTT is named as P21.
[0115] Said SEQ ID No.28: FAM-TTTTTTCCCTTTTT, named as P27.
[0116] The SEQ ID No.32: FAM-TTTTTTTTTTTTTTTT is named as P31.
[0117] 2. An aqueous solution of single-layer graphene oxide nanosheets with a concentration of 1 mg / mL;
[0118] 3. Nine bacterial suspensions with a McFarland turbidity of 4, as follows:
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention provides probes and method to identify bacteria and application of the probe. Nucleotide sequences of the probes are shown as SEQ ID NO. 1-32. The 32 specific DNA fluorescent probes aredesigned ingeniously; grapheneoxidenanosheet is used to carry out fluorescencequenching; mode recognition software is used to perform data processing; reaction conditions and steps are optimized; abacteria identifying system is finally established. The steps and conditions are of good synergy. The method herein is simple and quick to perform, has improved identifying efficiency and accuracy, and has a promising application prospect and good market value.
Description
technical field [0001] The invention relates to the technical field, in particular to a probe for identifying bacteria and its identification method and application. Background technique [0002] Bacterial identification is of great importance in medicine and industry. In medicine, to cure a patient infected by bacteria, doctors need to first know what kind of bacteria the patient is infected with and what kind of antibiotics the bacteria is sensitive to, so that they can choose the right drug to kill it. In industry, many products must be tested for bacteria before they can be sold, and this is especially strict in the food industry. Currently, commonly used bacterial identification methods have many limitations, such as long incubation time, professional experimenters, and expensive instruments. [0003] Graphene nanosheets are a material composed of single-layer atomic plane structure graphene stacks, which have unique quantum effects, excellent dispersion properties in...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More