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Coix seed triglyceride component quantitative analysis multi-components by single marker method

A technology of triglycerides and determination methods, which is applied in the field of determination of triglycerides in coix seed medicinal materials, extracts and preparations. It can solve the problem of inability to detect multiple components at the same time.

Inactive Publication Date: 2018-11-13
ZHEJIANG KANGLAITE PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the existing technology, the detection is mainly for some components in Kanglaite injection, and it is impossible to detect multiple components at the same time

Method used

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  • Coix seed triglyceride component quantitative analysis multi-components by single marker method
  • Coix seed triglyceride component quantitative analysis multi-components by single marker method
  • Coix seed triglyceride component quantitative analysis multi-components by single marker method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Determination method of coix seed decoction pieces:

[0077] Chromatographic conditions and system suitability test using Agilent Poroshell 120EC-C8 chromatographic column (3×100mm, 2.7μm), with methanol-acetonitrile (95:5) as the mobile equipotential elution, column temperature: 20°C, flow rate: 0.3mL / min, detected by an evaporative light scattering detector. The number of theoretical plates should not be less than 5000 based on the triolein peak.

[0078] Preparation of Reference Substance Solution Take an appropriate amount of triolein reference substance, accurately weigh it, add dichloromethane-methanol (1:9, v / v) to make a solution containing 80 μg per 1 mL, and obtain the solution.

[0079] The preparation of need testing solution takes about 0.5g of this product powder (through 65 mesh sieves), accurately weighs, puts in the Erlenmeyer flask with stopper, accurately adds methanol 50mL, seals up, weighs, and ultrasonically processes (power 300W, Frequency 40kH...

Embodiment 2

[0082] Content determination method of coix seed extract:

[0083] Coix seed oil sample preparation: take 50 mg of coix seed oil, weigh it accurately, put it in a 50 mL volumetric flask, add dichloromethane-methanol (1:9, v / v) to make a solution containing 1 mg per 1 mL, shake well, and have to.

[0084] Preparation of reference substance solution: Take an appropriate amount of triolein (OOO) reference substance, weigh it accurately, add dichloromethane-methanol (1:9, v / v) to make a solution containing 80 μg per 1 mL, shake well , that is.

[0085] Chromatographic conditions: Chromatographic column: Agilent Poroshell 120EC-C8 (3×100mm, 2.7μm), column temperature: 30°C; elute with methanol-acetonitrile (9:1) as the mobile equipotentiality, and the flow rate is 0.3mL / min. The sample volume is 5 μL, and the detection parameters of the evaporative light scattering detector (ELSD-LT II) are: temperature 45°C, gain value 11, filter value 10.

[0086] Determination method: Precise...

Embodiment 3

[0088] Content determination method of coix seed preparation:

[0089] Coix seed injection sample preparation: take 100 mg of coix seed injection, accurately weigh it, put it in a 10 mL volumetric flask, add dichloromethane-methanol (1:9, v / v) to make a solution containing 10 mg per 1 mL, and shake well , that is.

[0090] Preparation of reference substance solution: Take an appropriate amount of triolein (OOO) reference substance, weigh it accurately, add dichloromethane-methanol (1:9, v / v) to make a solution containing 0.08mg per 1mL, shake Uniform, that is.

[0091] Chromatographic conditions: Chromatographic column: Agilent Poroshell 120EC-C8 (3×100mm, 2.7μm), column temperature: 25°C; elute with methanol-acetonitrile (95:5) as the mobile equipotentiality, and the flow rate is 0.3mL / min. The sample volume is 5 μL, and the detection parameters of the evaporative light scattering detector (ELSD-LT II) are: temperature 45°C, gain value 11, filter value 10.

[0092] Determi...

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Abstract

The invention relates to a coix seed triglyceride component quantitative analysis multi-components by single marker method. According to the coix seed triglyceride component quantitative analysis multi-components by single marker method, high performance liquid chromatography is adopted, wherein Agilent Poroshell 120EC-C8 chromatographic column is adopted, methanol-acetonitrile is taken as a mobile phase for isocratic elution, column temperature is controlled to be 25 DEG C, flow velocity is controlled to be 0.3ml / min, an evaporation light scattering detector is adopted in detection, the theoretical plate number is calculated based on glyceryl trioleate peaks to be 5000 or higher. The coix seed triglyceride component quantitative analysis multi-components by single marker method can be used for measuring the contents of seven triglyceride components.

Description

Technical field: [0001] The invention relates to a detection method for medicines, in particular to a coix seed medicinal material, an extract and a preparation method for the determination of triglyceride components by one measurement and multiple evaluations. Background technique: [0002] Coix lacryma-jobi L.var.ma-yuen (Roman.) Stapf] belongs to the Gramineae Coix genus. The shelled seeds are used as medicine, and the name of the medicinal material is "coix kernel". Coix seed was first recorded in "Shen Nong's Materia Medica", which is not only a commonly used bulk medicinal material, but also a common food in daily life. As a medicine, Coix Seed has anti-tumor, hypoglycemic, blood lipid-lowering, immune-boosting and analgesic effects. Currently, Kanglaite Injection, which uses Coix Seed Glyceride as the main active ingredient, is already on the market. [0003] Coix Seed Extract: [0004] The extraction methods of coix seed oil include traditional organic solvent extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 李萍陈君王建成张朝军廖时余
Owner ZHEJIANG KANGLAITE PHARM CO LTD
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