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Ratio-type nano photoacoustic detection probe and preparation method and application thereof

A detection method and nano-light technology, applied in the field of probes, can solve the problems of poor selectivity and low sensitivity of detection technology, and achieve the effect of good application prospect and high sensitivity

Active Publication Date: 2018-11-16
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a ratio-type nano-photoacoustic detection probe and its preparation method and application, aiming at solving the problem of existing ONOO - Problems with poor selectivity and low sensitivity of detection techniques

Method used

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  • Ratio-type nano photoacoustic detection probe and preparation method and application thereof
  • Ratio-type nano photoacoustic detection probe and preparation method and application thereof
  • Ratio-type nano photoacoustic detection probe and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Synthesis of ratiometric nanophotoacoustic detection probes (nanonaps)

[0053] First, place 10mL of 10% (w / w) F127 in an ice-water bath, add 1mL of ONc and IR780 in dichloromethane dropwise to F127, and continue to stir for about 4 minutes, and then sonicate for 6 minutes; Then put the mixed solution at room temperature and continue to stir for 5 hours to obtain the precursor solution of the ratiometric nano-photoacoustic detection probe; The filter tube was centrifuged at 4°C at 4800xg, once every 30 minutes, washed 4 times with deionized water to remove free F127, and centrifuged until about 200 μL of liquid remained in the filter tube to obtain a ratiometric nano-photoacoustic detection probe.

[0054] The route and mechanism diagram of the synthetic ratiometric nano-photoacoustic detection probe, the corresponding TEM image, the ultraviolet-near infrared absorption and photoacoustic spectrum diagram are as follows: figure 1 shown. figure 1 Middle (a) s...

Embodiment 2

[0055] Example 2: Evaluation of in vitro sensitivity detection of ratiometric nanophotoacoustic detection probes

[0056] Evaluation of ratiometric nanophotoacoustic detection probes for different concentrations of ONOO - the response to. In the UV-NIR absorption detection, prepare 8 groups of the same nanoprobe solution (3 μg / mL ONc), add different concentrations of ONOO - (0-150nM), within 30 minutes, quickly read the absorption spectrum of each group of solutions at 400-1000nm, compare the changes in the absorption ratio (A863 / A780) of different groups at 863nm and 780nm, and use ONOO - Concentration is the abscissa, and A863 / A780 is the ordinate to draw a linear relationship curve between the two; in the process of photoacoustic signal detection, prepare 8 groups of the same nanoprobe solution (25μg / mL ONc), add different concentrations of ONOO - (0-150nM), measure the absorption spectrum of each group solution at 680-970nm within 30 minutes, compare the changes of the ...

Embodiment 3

[0058] Example 3: Evaluation of specific detection of ratiometric nanophotoacoustic detection probes

[0059] Compare the responsiveness of the ratiometric nano-photoacoustic detection probes to different reactive oxygen species (ROS) or reactive nitrogen species (RNS), and evaluate the nano-probes prepared in this example for ONOO - Specificity of detection. Prepare 11 groups of nanoprobe solutions (3 μg / mL) with the same concentration, the first group is used as the blank control group, and 1 μM ONOO is added to the remaining groups - , 1μM hypochlorite (ClO - ), 50μM hydrogen peroxide (H 2 o 2 ), 50 μM hydroxyl radicals (·OH), 50 μM singlet oxygen molecules ( 1 o 2 ), 50 μM nitric oxide radical (NO ), 50 μM superoxide radical (O 2 · - ), 50 μM of oxidative radicals (ROO ), 50 μM of L-cysteine ​​(Cys) and 50 μM of L-arginine (L-Arg), in order to quickly detect the effect of different groups of nanoprobes on ROS / RNS The responses of each group of solutions were placed...

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Abstract

The invention discloses a ratio-type nano photoacoustic detection probe and a preparation method and an application thereof. The ratio-type nano photoacoustic detection probe comprises poloxamer, anda naphthalocyanine dye and a heptamethine cyanine fluorescent small molecule supported on the poloxamer. The probe of the invention has high sensitivity and can specifically react with the peroxynitrite anion to change the ultraviolet-to-infrared absorption intensity ratio (A863 / A780) of the probe at 863 and 780 nm and change the photoacoustic signal ratio (PA860 / PA775) at 860 and 775nm, and thereby achieves selective detection and imaging of peroxynitrite anion in tumor parts of animals. In addition, the preparation process of the invention is simple, no complex and expensive apparatus is required to prepare the probe, and the preparation is simple and easy.

Description

technical field [0001] The invention relates to the technical field of probes, in particular to a ratio-type nano-photoacoustic detection probe and its preparation method and application. Background technique [0002] In vivo peroxynitrite anion (ONOO - ) is one of the reactive oxygen species (ROS) that regulates the basic functions of organisms. It is formed by superoxide anion radicals (O 2- ) and nitric oxide (NO) are rapidly combined to form. Specifically, it can readily penetrate cell membranes and oxidize a series of key biomolecules, such as proteins, lipids, nucleic acids, glycogen, iron-sulfur clusters, and thiols. Therefore, ONOO - Plays a key role in many diseases such as vascular disease, diabetes, cardiomyopathy, neurodegenerative disease, cerebral ischemia-reperfusion injury and inflammatory lung disease. According to statistical estimates, 15-20% of cancers are preceded by and chronic inflammation is accompanied by ONOO - increase. Therefore, the develop...

Claims

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Application Information

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IPC IPC(8): G01N21/17
CPCG01N21/1702
Inventor 黄鹏覃夏灵林静
Owner SHENZHEN UNIV
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