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Two active peptides derived from mudskipper with ace inhibitory activity

A peptide-inhibiting and anti-hypertensive technology, applied in the direction of peptides, can solve the problems of increased cost, missed screening of highly active peptide fragments, time-consuming and labor-intensive problems, etc.

Active Publication Date: 2021-08-06
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, based on traditional biological enzymatic hydrolysis, chromatographic multi-dimensional separation and structural identification, it not only increases the cost but also takes time and effort, and at the same time, it will cause some high activity peptide fragments to be missed in the screening.
Therefore, it becomes a constraint for efficient screening to identify peptides with ACE inhibitory activity.

Method used

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  • Two active peptides derived from mudskipper with ace inhibitory activity
  • Two active peptides derived from mudskipper with ace inhibitory activity
  • Two active peptides derived from mudskipper with ace inhibitory activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The screening of embodiment 1 raw material

[0015] The present invention searches for protein sequences related to marine protein sources from the NCBI (https: / / www.ncbi.nlm.nih.gov / protein / XP_020776504) database, and selects mudskipper actin as the raw material protein of ACE inhibitory peptides . The accession number is XP_020776504, which contains 6578 amino acids.

Embodiment 2 3

[0016] The screening of embodiment 2 tripeptide IDF and EGF

[0017] Pepsin (pH 1.3) and Trypsin were used in ExPASy PeptideCutter (https: / / web.expasy.org / peptide_cutter / ) to perform online hydrolysis of mudskipper actin protein (accession: XP_020776504) to obtain peptide sequences containing different amino acids , and screen out all peptide sequences containing three amino acids. The activity scores of these tripeptides were carried out in PeptideRanker (http: / / bioware.ucd.ie / ~compass / biowareweb / Server_pages / Peptid eranker.php), and a total of 17 tripeptides with activity scores higher than 0.55 were screened out. At the same time, the water-soluble Innovagen (http: / / www.innovagen.corn / proteomics-tools) and toxicity ToxinPred (http: / / crdd.osdd.net / raghava / / toxinpred / ) of these 17 peptides were predicted respectively, The tripeptides with good water solubility and non-toxicity were screened out. The peptide sequences, activity scores, water solubility and toxicity predictio...

Embodiment 3

[0022] Example 3 In Vitro Activity Verification

[0023] The ACE-inhibiting peptide is obtained through online screening and molecular docking of mudskipper concomitant actin. In order to verify the ACE-inhibiting activity of the IDF and EGF of the present invention, high-performance liquid chromatography is used.

[0024] 1. Instruments and chemicals: C18 liquid chromatographic column, Shimadzu LC-2030 high performance liquid chromatograph, hippuryl-histidyl-leucine (HHL), hippuric acid (HA), angiotensin converting enzyme (ACE) was purchased from Sigma, and acetonitrile, methanol and trifluoroacetic acid (TFA) were purchased from Fisher Scientific. All reagents were chromatographically pure.

[0025] 2. High-performance liquid chromatography analysis: Take HHL substrate solution, add inhibitors and mix evenly, preheat in 37°C constant temperature water bath for 3 to 5 minutes, then add ACE solution and mix thoroughly, and react immediately after 30 minutes at 37°C, and at th...

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Abstract

The invention provides two mudskipper-derived active peptides with ACE-inhibiting activity, the amino acid sequences of which are IDF and EGF respectively; the invention obtains the ACE-inhibiting tripeptide sequence by means of an in vivo digestive enzyme simulation system and guided by a specific target, and the method has It has the advantages of strong characteristics and high sensitivity; it also involves the action site where the two peptides bind to angiotensin converting enzyme and inhibit its activity. It provides a new method for the development of marine resource food, health products and medicines with antihypertensive properties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to two active peptides derived from mudskippers with ACE inhibitory activity. Background technique [0002] Bioactive peptides are specific protein fragments that improve human health by effectively promoting body functions or states. These bioactive peptides are inactive in the parent protein, but are released by proteolysis or fermentation to confer activity. In vivo, these bioactive peptides can exert a variety of activities, not only to improve nutrient utilization, but also to affect the cardiovascular, endocrine, immune and nervous systems. Angiotensin-converting enzyme (ACE) inhibitory peptides are usually a class of small peptide substances that can inhibit ACE activity. They are often combined with ACE in different ways to inhibit the hydrolysis of inactive angiotensin I to angiotensin II catalyzed by ACE. function, thereby lowering blood pressure. [0003] my country's...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083C07K5/093
CPCC07K5/0808C07K5/0819
Inventor 于志鹏樊玥赵文竹张馨月
Owner BOHAI UNIV
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