Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins

A technology for Newcastle disease virus and antigenic epitope is applied in the field of antigenic epitope of Newcastle disease virus matrix protein, which can solve the problems of less disclosure of technology and achieve the effect of simple and convenient identification system.

Active Publication Date: 2018-11-20
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

NDV has only one serotype, and the accurate epitope of M protein can be used as a biological marker to lay the foundation for the diagnosis of Newcastle disease and the development of marked vaccines, but there are few related technologies disclosed at present

Method used

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  • Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins
  • Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins
  • Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins

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Embodiment

[0047] A method for identifying the dominant antigenic epitope of M protein B cells of Newcastle disease virus LaSota strain is as follows:

[0048] Step 1: NDV LaSota strain M protein (amino acid sequence is SEQ ID NO.7)) is carried out secondary structure analysis with DNAStar Protean software; Use online database Immune Epitope Database (IEDB), ABCpred, Bcepred comprehensively to LaSota strain M protein Predict B cell epitopes; use the following seven prediction schemes: hydrophilicity scheme, protruding surface and corner scheme, surface accessibility scheme, flexibility scheme, polarity scheme, antigenicity scheme; as shown in Figure 1, M The protein has abundant α-helix, β-sheet and turn structures, strong hydrophobicity, high surface accessibility and antigenic index; and there are about 9 linear epitopes with high scores and stable existence in the M protein, of which The locations are as follows: 28-39, 48-56, 77-85, 118-126, 157-169, 223-233, 244-253, 296-311 and 345...

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Abstract

The invention discloses antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus (NDV) matrix (M) proteins, and relates to the technical field of biology. Theantigenic epitopes of the NDV M proteins provided by the invention has a sequence 1 of 77MIDDKP82 and a sequence 2 of 354HTLAKYNPFK363. B cell dominant linear epitopes of the two NDV M proteins provided by the invention can be used for detection antigen of serological analysis of NDV, and can be used as markers for constructing marker vaccines of the NDV; sequences of the B cell dominant linear epitopes of the two M proteins provided by the invention has a relatively high conservative property in the M proteins of all genotypes of the NDV; and epitope regions 77-82 and 354-363 of the M proteins are applicable to all NDV strains.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to an antigenic epitope, antibody, identification method and application of a matrix protein of Newcastle disease virus. Background technique [0002] Newcastle Disease (ND) is an acute, highly lethal infectious disease caused by Newcastle Disease Virus (NDV) and characterized by damage to the respiratory tract, digestive tract and nervous system of poultry. NDV is a single-stranded negative-sense RNA virus belonging to the genus Avian Paramyxovirus in the family Paramyxoviridae. The full length of the NDV genome is about 15.2 kb, similar to other paramyxoviruses, and its gene encoding includes nucleoprotein (NP), phosphoprotein (phosphoprotein, P), matrix protein (matrix protein, M), fusion protein (fusion protein) , F), six proteins including hemagglutinin-nueraminidase (HN) and large polymerase protein (RNA-dependent RNApolymerase or large polymerase, L), the genome ends with 3'...

Claims

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Application Information

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IPC IPC(8): C07K14/125G01N33/68G01N33/569C07K16/10A61K39/17A61P31/14
CPCA61K39/00A61P31/14C07K14/005C07K16/1027C12N2760/18122G01N33/56983G01N33/6854G01N33/6878G01N2333/125G01N2469/10G01N2469/20
Inventor 萧飒毕友坤杨增岐陈鸿军王文彬金忠元
Owner NORTHWEST A & F UNIV
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