Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins

A technology for Newcastle disease virus and antigenic epitope is applied in the field of antigenic epitope of Newcastle disease virus matrix protein, which can solve the problems of less disclosure of technology and achieve the effect of simple and convenient identification system.

Active Publication Date: 2018-11-20
NORTHWEST A & F UNIV
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NDV has only one serotype, and the accurate epitope of M protein can be used as a biological marker to lay the foundation for the diagnosis of Newcastle disease and the development of marked vaccines, but there are few related technologies disclosed at present

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins
  • Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins
  • Antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus matrix proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] A method for identifying the dominant antigenic epitope of M protein B cells of Newcastle disease virus LaSota strain is as follows:

[0048] Step 1: NDV LaSota strain M protein (amino acid sequence is SEQ ID NO.7)) is carried out secondary structure analysis with DNAStar Protean software; Use online database Immune Epitope Database (IEDB), ABCpred, Bcepred comprehensively to LaSota strain M protein Predict B cell epitopes; use the following seven prediction schemes: hydrophilicity scheme, protruding surface and corner scheme, surface accessibility scheme, flexibility scheme, polarity scheme, antigenicity scheme; as shown in Figure 1, M The protein has abundant α-helix, β-sheet and turn structures, strong hydrophobicity, high surface accessibility and antigenic index; and there are about 9 linear epitopes with high scores and stable existence in the M protein, of which The locations are as follows: 28-39, 48-56, 77-85, 118-126, 157-169, 223-233, 244-253, 296-311 and 345...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus (NDV) matrix (M) proteins, and relates to the technical field of biology. Theantigenic epitopes of the NDV M proteins provided by the invention has a sequence 1 of 77MIDDKP82 and a sequence 2 of 354HTLAKYNPFK363. B cell dominant linear epitopes of the two NDV M proteins provided by the invention can be used for detection antigen of serological analysis of NDV, and can be used as markers for constructing marker vaccines of the NDV; sequences of the B cell dominant linear epitopes of the two M proteins provided by the invention has a relatively high conservative property in the M proteins of all genotypes of the NDV; and epitope regions 77-82 and 354-363 of the M proteins are applicable to all NDV strains.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to an antigenic epitope, antibody, identification method and application of a matrix protein of Newcastle disease virus. Background technique [0002] Newcastle Disease (ND) is an acute, highly lethal infectious disease caused by Newcastle Disease Virus (NDV) and characterized by damage to the respiratory tract, digestive tract and nervous system of poultry. NDV is a single-stranded negative-sense RNA virus belonging to the genus Avian Paramyxovirus in the family Paramyxoviridae. The full length of the NDV genome is about 15.2 kb, similar to other paramyxoviruses, and its gene encoding includes nucleoprotein (NP), phosphoprotein (phosphoprotein, P), matrix protein (matrix protein, M), fusion protein (fusion protein) , F), six proteins including hemagglutinin-nueraminidase (HN) and large polymerase protein (RNA-dependent RNApolymerase or large polymerase, L), the genome ends with 3'...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/125G01N33/68G01N33/569C07K16/10A61K39/17A61P31/14
CPCA61K39/00A61P31/14C07K14/005C07K16/1027C12N2760/18122G01N33/56983G01N33/6854G01N33/6878G01N2333/125G01N2469/10G01N2469/20
Inventor 萧飒毕友坤杨增岐陈鸿军王文彬金忠元
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com