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Method for preparing microbial immobilized material

A technology for immobilizing and drying microorganisms, applied in the field of microorganisms, can solve the problems of easy disintegration and poor biosorption of carriers, and achieve the effects of improving biological activity, improving adsorption and prolonging the use time

Inactive Publication Date: 2018-11-20
常州市蒽盗钟情生物科技有限公司
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Problems solved by technology

[0003] Technical problem to be solved by the present invention: Aiming at the problems of poor biosorption and easy disintegration of the current carrier, to provide a preparation method of microorganism immobilization material

Method used

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preparation example Construction

[0021] A method for preparing a microorganism immobilization material, comprising the steps of:

[0022] (1) Take Penicillium marinum in mass ratio 2~4:1~3:10~20, add Penicillium simplex and sterile water to mix, and shake at 150r / min for 10~12h to get the mixed liquid, according to the mass parts In total, 8-12 parts of glucose, 1-4 parts of ammonium tartrate, 2-5 parts of potassium dihydrogen phosphate, 0.3-0.7 parts of magnesium sulfate pentahydrate, and 2-5 parts of yeast extract were mixed and sterilized at 121°C for 20 minutes to obtain For the base medium, take the mixed solution and inoculate it into the base medium according to the inoculum amount of 4-6%, cultivate it at 25-30°C and 150r / min for 5-7 days, centrifuge, take the precipitate and freeze-dry to obtain a freeze-dried product for future use ;

[0023] (2) Take ferric chloride hexahydrate, add ferrous chloride and deionized water according to the mass ratio of 5~8:2~3:20~25, stir and mix at 55~65°C for 20~30...

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Abstract

The invention discloses a method for preparing a microbial immobilized material and belongs to the technical field of microbes. The method disclosed in the invention comprises the following steps: taking marine penicillium janthinellum and penicillium simplicissimum as raw materials, adding glucose and other nutritive materials, and mutually twining to form bulb forms under the effect of applied shear force to obtain an immobilized matrix, so that the biological activity of the immobilized microbes is improved, the microbes are loaded in the immobilized matrix, the microbes are difficult to degrade, and the service time of the microbes is prolonged; taking ferric chloride hexahydrate and ferrous chloride as raw materials, adding sodium hydroxide, a silane coupling agent and amino hexamethylene glycol, and forming stable amido bonds. The stable performance of the immobilized material is improved and can be recycled based on magnetic substances, the microbes can be repeatedly loaded, andthe service life is prolonged. The problem that the conventional carrier is poor in biological adsorption property and easy to degrade is solved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of a microorganism immobilization material. Background technique [0002] In recent years, according to the characteristics of agricultural environmental pollutants, researchers have conducted systematic research on the treatment methods and processes of aquaculture sewage, using chemical methods and biological methods to adsorb nitrogen, phosphorus, heavy metal ions and organic molecules. , separation and degradation, and a single treatment method has achieved the goal of reduction and resource utilization to a certain extent, but it is difficult to achieve complete harmlessness and national emission standards. In the research process, activated carbon is usually used as an adsorbent for sewage pollutants. It can improve the color and turbidity of sewage, and it also contains macropores, mesopores and micropores, so it is suitable for smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12R1/80
CPCC12N11/14
Inventor 雷红军庄文琴李莉
Owner 常州市蒽盗钟情生物科技有限公司
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