Three-dimensional liquid drop detection system and detection method

Abstract

The invention discloses a three-dimensional liquid drop detection system which comprises a micro-fluidic chip, a point light source, an imaging unit, a scanning lens, and a three-dimensional liquid drop imaging unit, wherein liquid drops are distributed in the micro-fluidic chip in a three-dimensional state; light beams of the point light source are focused through a confocal detection system, andthe focused light beam is projected onto the liquid crops; the imaging unit is used for acquiring fluorescence signals reflected by the liquid drops to form a sectional image;; the three-dimensionalliquid drop imaging unit is used for processing all layers of sectional images of the liquid drops to form a three-dimensional liquid drop image. The scanning lens comprises a galvanometer or an objective lens moving in a mechanical way to move a focusing and projecting position of the liquid drops in the micro-fluidic chip by the point light source. The detection method disclosed in the inventiondetects and images the three-dimensionally distributed liquid drops through the point light source in a confocal manner, and is fast in quantitative detection, high in sensitivity and low in error; compared with the traditional detection for the single layer of liquid drops, the detection method increases the order of magnitude of samples treated within unit time, is clear in imaging and high inprecision, and is suitable for detection and analysis of proteins and nucleic acids and biopsy and early clinical diagnosis of tumors.

Description

technical field [0001] The invention relates to a three-dimensional droplet detection system and detection method, which belongs to the technical field of biochip detection, and is especially suitable for quantitative detection of protein and nucleic acid, as well as tumor biopsy and early clinical diagnosis. Background technique [0002] The high-throughput screening system originated from the research of drug screening. It mainly uses 96 or 384-well plates as arrayed reactors. Liquid distribution and sample mixing are performed by automated robots, which can achieve a screening throughput of at least 10,000 samples per day. Due to its powerful The application range of high-throughput screening technology has expanded from drug screening to biology, medicine, chemistry and other scientific fields, and research has focused on the miniaturization of high-throughput screening systems. [0003] The generation of micro-droplets is mainly how to implement a large enough force to ...

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Problems solved by technology

[0005] In order to improve the detection accuracy, those skilled in the art focus on generating several micro-droplets in a single-layer tiling or single separation state, and strive to improve the droplet generation accuracy and reduce the droplet volume, so as to effectively reduce the high-throughput screening. Medium sample / actual consumption, in order to save experimental costs, such as Chinese patent 20121589055.1 discloses a method of using an automated micro-droplet array screening system with pico-upgrade precision; use existing microfluidic chips to make single-layer or For quantitative detection and analysis of a single droplet, the separation process is complex and the separation efficiency is low. When used for detection, the detectable items are single and the number of detections per unit time is limited; The number of times will be more, and it will increase the detection workload while improving a certain detection accuracy.

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