A tissue scaffold with double-layer structure and its preparation method
A double-layer structure, tissue repair technology, applied in the field of medical devices, can solve the problems of decreased treatment effect, unclear function difference between layers, unclear double-layer structure, etc., and achieves good mechanical properties, easy operation, and interlayer connection. tight effect
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[0020] The second aspect of the present invention provides a method for preparing a tissue repair scaffold with a double-layer structure, wherein the method includes the following steps: (1) preparing a first solution containing a first cross-linkable component; (2) preparing a second solution comprising a second crosslinkable component, and in a mixed solution consisting of said first solution and said second solution, said second solution is relatively weak in foaming ability relative to said first solution And / or the defoaming speed is relatively fast; (3) the first solution is foamed to obtain a foaming first solution; (4) the second solution is added to the foaming first solution and the whole system is foamed to obtain a foaming mixed solution; (5) adding a cross-linking agent to the foaming mixed solution to cause a cross-linking reaction to occur in the foaming mixed solution to obtain a cross-linking reaction mixed solution; (6) pouring the cross-linking reaction mixed...
Embodiment 1
[0038] First prepare respectively the 8% by weight gelatin solution (being the first solution) of 200ml and the 1.2% by weight chitosan solution (being the second solution) of 50ml, the gelatin solution of 8% by weight is placed in the constant temperature water bath of 50 ℃ , the setting stirring speed is 2500 rev / mins, after the foaming of mechanical stirring until the volume is 3 times of original, the stirring speed is reduced to 500 revs / mins, slowly add 1.2% by weight chitosan solution, after adding, continue to stir for 5 Minutes, then slowly add 20ml of 10% formaldehyde solution (i.e. cross-linking agent solution) to carry out cross-linking reaction while stirring, after adding 10% by volume of formaldehyde solution, continue to react for 15 minutes, then pour into the mold, stand still 5 minutes, put it into -80°C refrigerator for pre-freezing for 5 hours, place it in a freeze dryer, freeze-dry at -50°C for 24 hours, and demould after freeze-drying to obtain a rough ti...
Embodiment 2
[0040] First prepare respectively the 5% by weight gelatin solution (i.e. the first solution) of 200ml and the 1.5% by weight hyaluronic acid solution (i.e. the second solution) of 50ml, and place the 5% by weight gelatin solution in a constant temperature water bath at 50°C , setting stirring speed is 3500 revs / min, after mechanical stirring foaming until volume is original 4 times, stirring speed is reduced to 800 revs / min, slowly add 1.5% by weight chitosan solution, after adding, continue to stir for 5 Minutes, then slowly add 20ml of 10% by volume glutaraldehyde solution (ie cross-linking agent solution) while stirring to carry out cross-linking reaction, after adding 10% by volume of glutaraldehyde solution, continue to react for 15 minutes, then pour Put it into the mold, let it rest for 2 minutes, put it in a -80°C refrigerator for 5 hours, put it in a freeze dryer, freeze-dry it at -50°C for 24 hours, and demould after freeze-drying to obtain a rough tissue repair scaf...
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