A microbial treatment agent for degrading aflatoxin b1 and its preparation method and application

A technology of microbial treatment and aflatoxin, applied in the field of animal breeding, can solve the problems of incomplete detoxification, destruction of nutrition, and high cost, and achieve the effects of inhibiting the production of toxins, low cost, and easy operation

Active Publication Date: 2022-02-08
LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a microbial treatment agent for degrading aflatoxin B1 and its preparation method and application, aiming at the problems of aflatoxin B1 detoxification method destroying nutrition, incomplete detoxification and high cost

Method used

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  • A microbial treatment agent for degrading aflatoxin b1 and its preparation method and application
  • A microbial treatment agent for degrading aflatoxin b1 and its preparation method and application
  • A microbial treatment agent for degrading aflatoxin b1 and its preparation method and application

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preparation example Construction

[0039] The invention also discloses a preparation method of a microbial treatment agent for degrading aflatoxin B1, comprising the following steps:

[0040] Step 1. Preparation of probiotic complex:

[0041] Step 1.1, preparation of Saccharomyces cerevisiae culture solution: Inoculate Saccharomyces cerevisiae in Saccharomyces cerevisiae fermentation medium at an inoculum size of 4%-8% (V / V), at 25-30°C, 155-165r / min shaker culture, Harvest when cultured for 27 hours, the content of regulator bacteria is 1×10 9 CFU / ml;

[0042] Among them, the composition of Saccharomyces cerevisiae fermentation medium: glucose 20g, yeast powder 10g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, distilled water 1.0L, natural pH;

[0043] Step 1.2, the preparation of Acetobacter pasteurian culture fluid: inoculate Acetobacter pasteurii in the acetic acid bacteria fermentation medium by the inoculum size of 4%-8% (V / V), at 25-35 ℃, 95-105r / min Cultivate on a shaking table, harvest...

Embodiment 1

[0066] A preparation method of a microbial treatment agent for degrading aflatoxin B1, comprising the following steps:

[0067] Step 1. Preparation of probiotic complex:

[0068] Step 1.1, preparation of Saccharomyces cerevisiae culture medium: Inoculate Saccharomyces cerevisiae in the Saccharomyces cerevisiae fermentation medium at an inoculum size of 6% (V / V), culture at 29°C, 160r / min shaker, harvest when cultured for 27h, adjust The content of bacteria is 1×10 9 CFU / ml;

[0069] Among them, the composition of Saccharomyces cerevisiae fermentation medium: glucose 20g, yeast powder 10g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, distilled water 1.0L, natural pH;

[0070] Step 1.2, preparation of Acetobacter pasteurian culture fluid: inoculate Acetobacter pasteurianum in the acetic acid bacteria fermentation medium by the inoculum size of 6% (V / V), at 30 ℃, 100r / min shaker culture, to be cultivated for 36h When harvested, the content of regulator bacteria w...

Embodiment 2

[0084] A preparation method of a microbial treatment agent for degrading aflatoxin B1, comprising the following steps:

[0085] Step 1. Preparation of probiotic complex:

[0086] Step 1.1, preparation of Saccharomyces cerevisiae culture medium: Inoculate Saccharomyces cerevisiae in the Saccharomyces cerevisiae fermentation medium at an inoculum size of 4% (V / V), culture on a shaker at 30°C and 155r / min, harvest after 27 hours of cultivation, and adjust The content of bacteria is 1×10 9 CFU / ml;

[0087] Among them, the composition of Saccharomyces cerevisiae fermentation medium: glucose 20g, yeast powder 10g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, distilled water 1.0L, natural pH;

[0088] Step 1.2, preparation of Acetobacter pasteurian culture fluid: Inoculate Acetobacter pasteurianus in the acetic acid bacteria fermentation medium by the inoculum size of 4% (V / V), at 35 DEG C, 95r / min shaker culture, to be cultivated for 36h When harvested, the content ...

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Abstract

The invention discloses a microbial treatment agent for degrading aflatoxin B1 and its preparation method and application. The microbial treatment agent comprises a probiotic compound and aflatoxin B1 degrading enzyme with a volume ratio of 1:3-3:1 crude enzymes. The preparation method comprises the following steps: preparing a probiotic compound; preparing aflatoxin B1 degrading enzyme crude enzyme; mixing the probiotic compound and aflatoxin B1 degrading enzyme crude enzyme in a volume ratio of 1:3-3:1 Mixing is carried out to prepare a microbial treatment agent for degrading aflatoxin B1. The microbial treatment agent of the present invention can significantly alleviate the adverse effects of AFB1 on the production performance of chickens and pigs.

Description

technical field [0001] The invention belongs to the technical field of animal feeding and breeding, and in particular relates to a microbial treatment agent for degrading aflatoxin B1, a preparation method and application thereof. Background technique [0002] Aflatoxin B1 (Aflatoxin B1, abbreviated as AFB1) is a derivative of dihydrofuran oxinone, which contains a difuran ring and an oxinone (coumarin). In the natural food of humans and animals, AFT (Aflatoxin) exists widely, and AFB1 is the most common and most harmful. AFB1 has strong toxicity to humans and some animals. For example, the hazards to poultry mainly include: Reduced feed intake, weight gain and feed conversion rate, infertility, abortion, pulmonary edema, increased susceptibility to diseases, etc.; the main hazards to pigs are: reduced feed intake, weight gain and feed conversion rate, infertility and abortion, Pulmonary edema, increased susceptibility to blight, etc.; hazards to dairy cows mainly include: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12N9/00A23L5/20C12R1/865C12R1/10C12R1/02C12R1/685C12R1/845
CPCA23L5/25A23L5/28C12N1/18C12N1/20C12N9/00
Inventor 张兴良夏锡兰钟秀
Owner LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
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