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Method for improving yield of vitamin B12 based on dissolved oxygen regulation

A vitamin and B12 technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, difficulty in purification, and many by-products, and achieve the effect of increasing production

Inactive Publication Date: 2018-11-30
GUANGJI PHARMA MENGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Production of vitamin B by chemical synthesis 12 The cost of the method is high, the by-products are many, and it is difficult to purify

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method of improving vitamin B based on dissolved oxygen regulation 12 The yield method includes the following steps:

[0023] 1) Cultivate vitamin B at a fermentation temperature of 32°C 12 The production strain of Pseudomonas denitrifica, the composition of the fermentation medium used is: sucrose 70g / L, corn steep liquor 45g / L, ammonium sulfate 0.5g / L, potassium dihydrogen phosphate 0.4g / L, cobalt chloride 0.06 g / L, magnesium oxide 0.4g / L, 5,6-dimethylbenzimidazole 0.04g / L, betaine 10g / L, zinc sulfate 0.05g / L, calcium carbonate 0.5g / L, arginine 0.0005 g / L and thiamine 0.00005g / L, the pH of the fermentation medium was 7.2; The stirring rate is controlled at 250rpm, the aeration rate is 1.2LPM, and the stirring rate is controlled at 100rpm in the middle and later stages of fermentation, and the aeration rate is 0.5LPM;

[0024] When the concentration of betaine in the fermentation medium was reduced to 5g / L, add betaine to maintain its concentration at 5g / L, and si...

Embodiment 2

[0028] A method of improving vitamin B based on dissolved oxygen regulation 12 The yield method includes the following steps:

[0029] 1) Cultivate vitamin B at a fermentation temperature of 32°C 12 The production strain Pseudomonas denitrification, the composition of the fermentation medium used is: sucrose 75g / L, corn steep liquor 50g / L, ammonium sulfate 0.8g / L, potassium dihydrogen phosphate 0.5g / L, cobalt chloride 0.06 g / L, magnesium oxide 0.6g / L, 5,6-dimethylbenzimidazole 0.05g / L, betaine 15g / L, zinc sulfate 0.06g / L, calcium carbonate 0.8g / L, arginine 0.001 g / L and thiamine 0.0001g / L, the pH of the fermentation medium was 7.2; The stirring rate is controlled at 300rpm, the aeration rate is 1.5LPM, the stirring rate is controlled at 150rpm, and the aeration rate is 0.6LPM in the middle and late stages of fermentation;

[0030] When the concentration of betaine in the fermentation medium was reduced to 6g / L, add betaine to maintain its concentration at 6g / L, and simultan...

Embodiment 3

[0034] A method of improving vitamin B based on dissolved oxygen regulation 12 The yield method includes the following steps:

[0035] 1) Cultivate vitamin B at a fermentation temperature of 32°C 12 The production strain of Pseudomonas denitrifica, the composition of the fermentation medium used is: sucrose 85g / L, corn steep liquor 60g / L, ammonium sulfate 1g / L, potassium dihydrogen phosphate 0.7g / L, cobalt chloride 0.08g / L, magnesium oxide 0.6g / L, 5,6-dimethylbenzimidazole 0.06g / L, betaine 20g / L, zinc sulfate 0.09g / L, calcium carbonate 1.2g / L, arginine 0.002g / L and thiamine 0.00025g / L, the pH of the fermentation medium is 7.4; the fermentation process is divided into the early stage of fermentation (0-70h period of fermentation) and the middle and late period of fermentation (71-180h period of fermentation). The stirring rate is controlled at 350rpm, the aeration rate is 2LPM, the stirring rate is controlled at 200rpm, and the aeration rate is 1LPM in the middle and late s...

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PUM

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Abstract

The invention belongs to the technical field of biological fermentation, and discloses a method for improving the yield of vitamin B12 based on dissolved oxygen regulation. The method comprises the following steps: (1) under the condition suitable for fermentation, culturing a production strain of vitamin B12, wherein a culture medium contains the following ingredients: 70-85 g / L of saccharose, 45-60 g / L of corn steep liquor, 0.4-0.7 g / L of potassium dihydrogen phosphate, 0.06-0.08 g / L of cobalt chloride, 10-20 g / L of glycine betaine, 0.0005-0.002 g / L of arginine, and 0.00005-0.0003 g / L of thiamine, at the earlier stage of fermentation, the stirring speed is controlled to be 250-400 rpm, the ventilation rate is controlled to be 1.2-2 LPM, at the middle and later stages of fermentation, thestirring speed is controlled to be 100-200 rpm, and the ventilation rate is controlled to be 0.5-1 LPM; and (2) separating and purifying vitamin B12 from the fermentation liquor. According to the method, by controlling the dissolved oxygen amount and the material adding amount in the fermentation liquor, the yield of vitamin B12 is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for increasing vitamin B based on dissolved oxygen regulation. 12 yield method. Background technique [0002] Vitamin B 12 Also known as cobalamin, it is an important nutritional factor for animals and humans. It is widely used in feed, food and medical and health fields. It has significant effects in many aspects, such as promoting the development and maturation of red blood cells, preventing pernicious anemia; maintaining Nervous system health; exists in the form of coenzymes, can increase the utilization rate of folic acid, promote the metabolism of carbohydrates, fats and proteins; can also promote protein synthesis, which plays an important role in the growth and development of infants; it can also eliminate restlessness, Concentrate, enhance memory and sense of balance, and vitamin B 12 Participate in the formation of a lipoprotein in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/42C12R1/38
CPCC12P19/42
Inventor 张广勇杨明玉王鑫李会
Owner GUANGJI PHARMA MENGZHOU
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