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Method for detecting succinylcholine chloride in food and metabolite of food

A technology for succinylcholine chloride and metabolites, which is applied in the field of detection, can solve the problems of unsatisfactory detection requirements, complicated pretreatment, inaccurate quantification, etc., and achieve the effects of easy promotion and use, high extraction efficiency, and good separation effect

Inactive Publication Date: 2018-11-30
刘思洁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the detection of succinylcholine chloride residues in animal-derived foods, the complexity of the animal-derived food matrix must be considered, and the requirements for its separation and enrichment are relatively high. However, the existing methods have complex pretreatment and quantitative Inaccurate and other issues, especially succinylcholine chloride in animal-derived foods will be metabolized into succinyl monocholine chloride in the body, so the existing methods cannot meet the requirements of succinylcholine chloride and succinylcholine chloride in animal-derived foods. Therefore, it is urgent to establish a detection technology for succinylcholine chloride and its metabolites in food of animal origin.

Method used

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  • Method for detecting succinylcholine chloride in food and metabolite of food
  • Method for detecting succinylcholine chloride in food and metabolite of food
  • Method for detecting succinylcholine chloride in food and metabolite of food

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Embodiment 1

[0044] The detection of succinylcholine chloride and its metabolite succinylcholine chloride residues in dog meat in embodiment 1

[0045] 1) Sample pretreatment

[0046] Weigh 5g of homogeneous sample (accurate to 0.01g), place it in a 50mL polypropylene centrifuge tube, add 100μL 25μg / mL paraoxon working solution, 5mL 0.1mol / L hydrochloric acid, 5mL extraction buffer, and homogenize After mixing by machine, vortex mixed for 10 minutes, centrifuged at 10,000 r / min for 10 minutes (temperature lower than 4°C), and the supernatant was taken for purification.

[0047] First activate the solid phase extraction column with 6mL methanol and 3mL extraction buffer, then take 5mL supernatant and load it on the solid phase extraction column, rinse the small column with 4mL eluent, fully drain it, and elute with 4mL methanol, Collect the eluate, blow the eluate to near dryness with nitrogen below 45°C, dissolve the residue with 1 mL of formic acid: acetonitrile: water (1:59:40), filter ...

Embodiment 2

[0072] The standard series of succinylcholine chloride and succinylcholine chloride with different concentrations were prepared with blank matrix solution, analyzed by UPLC-MS / MS, and the linear range, regression equation, correlation coefficient (r) and detection limit were obtained . Carry out sample processing by the method of the present invention, add the mixed standard solution of 3 levels of succinylcholine chloride and succinylcholine chloride of different contents in matrix sample, each addition concentration measures in parallel 6 times, measure precision and Accuracy.

[0073] 1) Linear range, regression equation, detection limit and quantification limit

[0074] The concentration of succinylcholine chloride and succinylcholine chloride was linear in the range of 1.5μg / L-15μg / L. When the sampling volume is 5.0 g and the constant volume is 1.0 mL, the detection limit of succinylcholine chloride and succinylcholine chloride is 0.2 μg / kg, and the quantification limit...

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Abstract

The invention discloses a method for detecting succinylcholine chloride and metabolite in food. A precise qualitative and quantitative method for determination of the succinylcholine chloride in animal-derived food and the metabolite succinylcholine chloride of the animal-derived food is established by reverse phase solid phase extraction-ultra high performance liquid chromatography-mass spectrometry / mass spectrometry technology. The established method provides a scientific basis for emergency treatment of food safety public health emergencies.

Description

technical field [0001] The invention belongs to the technical field of detection. Specifically relates to a method for detecting succinylcholine chloride and its metabolites in food. Background technique [0002] Succinylcholine Chloride (Succinylcholine Chloride), also known as Chlorosuccinylcholine, Succinylcholine, Succinylcholine, the chemical name is 2,2'-[(1,4-dioxo-1,4- Butylene)bis(oxygen)]bis[N,N,N-trimethylethylammonium]dihydrate, molecular formula is C 14 h 30 Cl 2 N 2 o 4 2H 2 O. Is a depolarizing muscle relaxant that produces a steady depolarization that causes skeletal muscle relaxation. After entering the body, it can be quickly hydrolyzed by pseudocholinesterase in the blood to the intermediate metabolite succinyl monocholine chloride. It is one of the earliest anesthetics discovered by human beings. In recent years, some lawbreakers have taken advantage of the fast acting and high toxicity of succinylcholine chloride to make darts to steal dogs or p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 刘思洁吴永宁张博何东阳石矛
Owner 刘思洁
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