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Method for increasing propagation coefficient of tufty seedlings of Lycopodium serratum

A technology of reproduction coefficient and Melaleuca tower, which is applied in the field of improving the reproduction coefficient of clump seedlings of Melaleuca, can solve the problems of low reproduction coefficient, difficulty in obtaining a large number of sterile explants, and failure to realize large-scale production, so as to achieve an increase in reproduction coefficient , Improve the success rate of sterilization and meet the requirements of large-scale production

Active Publication Date: 2018-12-04
合肥膳之纤生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Melaleuca tissue culture is restricted by two factors of low reproduction coefficient and difficulty in obtaining a large number of sterile explants, so far no large-scale production has been realized. The present invention aims to provide A kind of Melaleuca tissue culture method suitable for large-scale production

Method used

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  • Method for increasing propagation coefficient of tufty seedlings of Lycopodium serratum
  • Method for increasing propagation coefficient of tufty seedlings of Lycopodium serratum
  • Method for increasing propagation coefficient of tufty seedlings of Lycopodium serratum

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: Comparison of four different induction conditions of 6-BA, 6-BA+L-serine, 6-BA+blue light, 6-BA+L-serine+blue light on the induction rate of clustered seedlings of sterile explants and the propagation of clustered seedlings The effect of the coefficient.

[0024] (1) Sterilize the surface of wild Melaleuca sporophytes. Select robust wild Melaleuca sporophytes with a plant height of about 12cm, use a soft brush to dip in detergent in running water to clean the branches, leaves and roots, rinse off the residual detergent on the surface, and use 75 Soak in % ethanol solution for 1.0 min, 0.1% mercuric solution for 8 min, and 2% sodium hypochlorite solution for 20 min to sterilize the surface of the sporozoites, rinse them with sterile water for 5 times, and blot the surface moisture with sterile paper.

[0025] (2) Preparation of explants. Under sterile conditions, the sporophytes were cut close to the root, the length was 2.5cm, and the stem segment with 8 le...

Embodiment 2

[0034] Example 2: Comparing the induction rate of clustered seedlings and the reproduction coefficient of clustered seedlings of explants derived from wild sporophytes and explants derived from sterile clustered seedlings.

[0035] (1) Preparation of explants derived from wild sporophytes. Select the robust wild sporophytes of Melaleuca pagoda with a plant height of about 12cm, and perform operations such as surface sterilization, preparation of explants, adaptive cultivation of explants, and endophytic sterilization of explants according to the technical parameters in Example 1. , to prepare axenic explants from wild sporophyte sources.

[0036] (2) Preparation of sterile explants derived from sterile clustered seedlings. Select the robust aseptic clustering seedlings of Melaleuca pagoda with a plant height of about 12cm, and cut the stem section close to the root, 2.5cm in length and with 8 leaves under sterile conditions as an explant, and the upper incision of the explant...

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Abstract

The invention relates to a method for increasing the propagation coefficient of tufty seedlings of Lycopodium serratum. The method comprises, sterilizing the surface of sporophore of wild Lycopodium serratum, preparing explants, performing adaptive cultivation of the explants, performing sterilization on endophyte in the explants, performing induced proliferation cultivation on the tufty seedlings, performing growth cultivation of the tufty seedlings and other steps. The induced proliferation success rate of the sterile explant tufty seedlings reaches 90% or above, and the average propagationcoefficient of the tufty seedlings can reach 60 or above; and the sterile tufty seedlings obtained through cultivation can be directly used for preparing the sterile explants and carrying out cyclic induced proliferation of the tufty seedlings. Through the method, not only can the success rate of induced proliferation and the propagation coefficient of the tufty seedlings of Lycopodium serratum beimproved, but also a large number of the sterile explants can be obtained through once sterilization treatment; and no repeated sterilization operation is needed, the risk of infection is reduced, the work efficiency is improved, and the process is simplified; and the method is suitable for large-scale production of Lycopodium serratum.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for increasing the propagation coefficient of the clustered seedlings of Melaleuca pagoda. technical background [0002] Melaleuca, scientific name Huperzia serrata, is a perennial fern of the genus Huperzia in the family Huperzaceae. In the natural state, Melaleuca can reproduce in three ways: spore reproduction, spore reproduction and root branch reproduction. Huperzine A It is one of the main active ingredients of Melaleuca. Huperzine A is a reversible acetylcholinesterase inhibitor, which can increase the content of acetylcholine in the brain and has a good therapeutic effect on Alzheimer's disease. Although the artificial synthesis of huperzine A has achieved some results, the entire synthesis process The steps are cumbersome, the cost is high, the efficiency is low, and it is difficult to realize industrial production. Therefore, the main ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 赵宏伟
Owner 合肥膳之纤生物科技有限公司