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Broken Lactobacillus supercritical co 2 Static and dynamic method for synergistically extracting astaxanthin

A supercritical, lactobacillus technology, applied in separation methods, chemical instruments and methods, liquid solution solvent extraction, etc., can solve the problems of toxic organic solvent residues, difficult commercial application, and inability to be widely promoted, and achieves improved extraction efficiency, Easy operation and high extraction efficiency

Active Publication Date: 2019-08-20
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are a large amount of toxic organic solvent residues in this method, which cannot be widely promoted and is difficult to be commercially applied.

Method used

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  • Broken Lactobacillus supercritical co <sub>2</sub> Static and dynamic method for synergistically extracting astaxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Broken Lactobacillus Supercritical CO 2 The method for static and dynamic cooperative extraction of astaxanthin comprises the following steps:

[0042] The fermented Lactobacillus was dehydrated twice with 95% ethanol and once with absolute ethanol, each time for 20 minutes, and dehydrated to a dry state.

[0043] Take by weighing the bacterium powder of the lactobacillus after 110g drying, be 0.6 according to the mass ratio of bacterium powder and ball milling beads, get ball milling beads 6a, 12b, 18c altogether 183.4g (example: 6a means to get 6 ball milling beads of a specification, with this By analogy), the mass ratio of bacterial powder and ethanol is 1, weigh ethanol, mix it and add it to the ball mill tank, the temperature of the air cooler is set at 5°C, the speed of the ball mill is set at 700r / min, and the wall is broken by the ball mill for 2 hours. After completion, filter with an 18-mesh sieve to obtain the bacterial slurry, add 150 g of silicon dioxide ...

Embodiment 2

[0048] Broken Lactobacillus Supercritical CO 2 The method for static and dynamic cooperative extraction of astaxanthin comprises the following steps:

[0049] The fermented Lactobacillus was dehydrated twice with 95% ethanol and once with absolute ethanol, each time for 20 minutes, and dehydrated to a dry state.

[0050] Weigh 110g of dry bacteria, according to the mass ratio of bacteria powder and ball milling beads as 1, take ball milling beads 1a, 2b, 8c, 14d, 82e in total 110.0g (for example: 6a means take 6 ball milling beads of a specification, and so on) , the mass ratio of bacteria powder and ethanol is 0.5, weigh ethanol, mix it and add it to a ball mill tank, set the temperature of the air cooler to 5°C, and the speed of the ball mill to 400r / min, and break the wall by the ball mill for 3 hours. After completion, filter with a 18-mesh sieve to obtain the bacterial slurry, add 110g of silicon dioxide and mix uniformly and pour into the extraction kettle 6.

[0051] ...

Embodiment 3

[0055] Broken Lactobacillus Supercritical CO 2 The method for static and dynamic cooperative extraction of astaxanthin comprises the following steps:

[0056] The fermented Lactobacillus was dehydrated twice with 95% ethanol and once with absolute ethanol, each time for 20 minutes, and dehydrated to a dry state.

[0057] Take by weighing 90g of dry bacteria, according to the mass ratio of bacteria powder and ball milling beads is 0.6, get a total of 150.0g of ball milling beads 8b, 6c, 24d (example: 6a means getting 6 ball milling beads of a specification, and so on), bacteria powder and The mass ratio of ethanol is 0.7. Weigh the ethanol, mix it and add it to the ball mill tank. The temperature of the air cooler is set at 5° C., the speed of the ball mill is set at 650 r / min, and the wall is broken by the ball mill for 2 hours. After completion, filter with an 18-mesh sieve to obtain the bacterial slurry, add 210 g of silicon dioxide and mix evenly, and then pour it into the...

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Abstract

The invention relates to a method for static and dynamic synergistic extraction of astaxanthin by wall-broken lactobacillus supercritical CO2. The method comprises the following steps: adding the dried plant lactobacillus to ethanol, and after ball-milling wall-breaking, obtaining the lactobacillus bacteria slurry as an extraction raw material; mixing diatomite and lactobacillus bacteria slurry, and adding a mixture to an extraction vessel; setting the supercritical extraction temperature to 35-60 DEG C and the supercritical extraction pressure to 10-40 MPa, and flowing supercritical CO2 fromabove the extraction vessel, when the supercritical extraction temperature and the supercritical extraction pressure reach a set value and are stabilized, closing a valve (V2), and cutting off the extraction kettle and a separation vessel; and after the high-pressure solubilization static extraction is completed, regulating the valve and introducing the supercritical CO2 for dynamic synergistic extraction. The method is used for extracting astaxanthin from lactobacillus, has the advantages of low cost, simple operation steps, and high extraction rate, no other organic solvent consumption except ethanol is required, and the whole process is green, low energy consumption and suitable for commercial application.

Description

technical field [0001] The invention relates to the development and extraction of biological materials in the field of biotechnology, in particular to a method for statically and dynamically synergistically extracting astaxanthin with supercritical CO2 from Lactobacillus wall-breaking, and belongs to the field of fungal biotechnology. Background technique [0002] Astaxanthin is a lutein carotenoid that is widely found in various microorganisms and marine animals, such as in the cells of some algae, yeast and bacteria, or in the shells of crustaceans. The chemical name of astaxanthin is 3,3′-dihydroxy-4,4′-diketonyl-β,β′-carotene, molecular formula C 40 h 52 o 4 , the molar mass is 596.86g / mol. Astaxanthin is an unsaturated ketocarotenoid consisting of two terminal rings and a polyene chain. Astaxanthin not only exists in the form of stereoisomers, free state and esterified state, but also has geometric isomers. [0003] Astaxanthin has strong anti-tumor, anti-aging, pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24B01D11/04
CPCB01D11/0403C07C403/24C07C2601/16Y02P20/54
Inventor 江燕斌叶丽萍刘萧峰宋光均蹇华丽何俊杰
Owner SOUTH CHINA UNIV OF TECH
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