Breeding method for cinnamomum camphora
A technology of camphor tree and tree body, which is applied in the field of camphor tree breeding, can solve the problems of slow growth of camphor tree, long cultivation period, and breeding of camphor tree, and achieve the goal of improving survival rate of breeding, reducing cultivation period and quantity, and good cultivation effect Effect
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Embodiment 1
[0020] A kind of propagation method of camphor tree, comprises the following steps:
[0021] (1) cultivation body selection: reach about 20 ℃ in autumn temperature, select good seed quality, tree body health, no damage by disease and insect, and the half-lignified twig of the year on the camphor tree seedling of 1 year is made cultivation body;
[0022] (2) Detoxification of the culture body: Rinse the selected culture body with clean water, disinfect it with 75% alcohol alcohol for 30 seconds in the aseptic laboratory, and then sterilize it with 0.1% mercuric chloride for 5 minutes. Rinse with water 3 times, dry the filter paper and set aside;
[0023] (3) value-added culture: the culture body is cut into 1.5cm of the segment with axillary buds, and then cultured on the culture medium, the callus induction medium is MS, and when the bud tip grows 2cm, it is inoculated into the culture medium for value-added culture; This step is not required when the selected culture body is...
Embodiment 2
[0027] A kind of propagation method of camphor tree, comprises the following steps:
[0028] (1) culture body selection: reach about 20 ℃ in autumn temperature, select good seed property, tree body health, no damage by disease and insect, the half lignified twig of the year on the camphor tree seedling of 1.5 years is made culture body;
[0029] (2) Detoxification of the culture body: rinse the selected culture body with clean water, disinfect it with 75% alcohol alcohol for 30 seconds in the aseptic laboratory, and then sterilize it with 0.1% mercuric chloride for 8 minutes. Rinse with water 3 times, dry the filter paper and set aside;
[0030] (3) Value-added culture: the culture body is cut into 1.8cm sections with axillary buds, and then cultured on the culture medium, the callus induction medium is MS, and when the bud tip grows 2cm, it is inoculated into the culture medium for value-added culture; This step is not needed when the selected culture body is the shoot tip a...
Embodiment 3
[0034] A kind of propagation method of camphor tree, comprises the following steps:
[0035] (1) cultivation body selection: reach about 20 ℃ in autumn temperature, select good seed property, tree body health, no damage by disease and insect, the half-lignified twig of the year on the camphor tree seedling of 2 years is made cultivation body;
[0036] (2) Detoxification of the culture body: rinse the selected culture body with clean water, disinfect it with 75% alcohol alcohol for 30 seconds in the aseptic laboratory, and then sterilize it with 0.1% mercuric chloride for 10 minutes. Rinse with water 3 times, dry the filter paper and set aside;
[0037] (3) Value-added culture: the culture body is cut into 2 cm sections with axillary buds, and then cultured on the culture medium. The callus induction medium is MS, and when the bud tip grows 2 cm, it is inoculated into the culture medium for value-added culture; This step is not required when the selected culture body is the sh...
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