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Application of four plant nac transcription factor nac089 genes and their expression vectors in soybean and Nicotiana benthamiana

A technology of transcription factors and expression vectors, applied in the field of genetic engineering, can solve problems such as ineffectiveness and achieve the effect of improving resistance

Inactive Publication Date: 2020-10-16
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phytophthora capsici belongs to oomycetes. Although this kind of pathogenic bacteria is similar to fungi in morphology, it is closely related to diatoms and cyanobacteria in evolution, and belongs to the kingdom of anthracnose. Therefore, general fungal fungicides are often effective against Phytophthora soybean and Other oomycetes are invalid

Method used

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  • Application of four plant nac transcription factor nac089 genes and their expression vectors in soybean and Nicotiana benthamiana
  • Application of four plant nac transcription factor nac089 genes and their expression vectors in soybean and Nicotiana benthamiana

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Experimental program
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Effect test

Embodiment 1

[0017] Example 1 Transient expression of NbNAC089-1, NbNAC089-2, GmNAC089-1, and GmNAC089-2 in Nicotiana benthamiana improves the resistance of Nicotiana benthamiana to Phytophthora capsici

[0018] 1. Tested strains

[0019] The tested strain Phytophthora capsici LT263 was preserved by the Plant-Phytophthora Interaction Laboratory of the Department of Plant Diseases of Nanjing Agricultural University (a conventional strain widely used by those skilled in the art). The strains were stored in 10% V8 solid medium at a storage temperature of 10°C.

[0020] 2. Preparation of Nicotiana benthamiana seedlings for testing

[0021] Nicotiana benthamiana were sown in plastic pots (d=10cm) filled with vermiculite (2-4mm), and grown in a greenhouse with 14h light (strong light irradiation) / 10h darkness. Let it grow for 7 days, after growing two true leaves, transplant it to the culture soil with vermiculite black soil volume ratio of 5:1 and grow it for 30 days, and take the leaves at t...

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Abstract

The invention belongs to the field of genetic engineering, and discloses four plant NAC transcription factor NAV089 genes in Glycine max and Nicotiana benthamiana and an application of expression vectors thereof. The Nicotiana benthamiana is taken as an example. In the Nicotiana benthamiana, the NAV089 genes in the Glycine max and the Nicotiana benthamiana are instantaneously expressed, and so, the resistance of the Nicotiana benthamiana to phytophthora can be significantly improved. In the current situation without a particularly good method for controlling the phytophthora, important significance is provided for cloning of the NAC089 genes and for crop varieties with phytophthora resistance cultivated by means of genetic engineering.

Description

technical field [0001] The invention belongs to the field of genetic engineering and discloses the application of four plant NAC transcription factor NAC089 genes in soybean and Nicotiana benthamiana and their expression vectors in plant resistance to Phytophthora infection. Background technique [0002] Phytophthora capsici is a broad-spectrum pathogen that can cause serious damage to various economic crops such as peppers and tobacco. Phytophthora capsici belongs to oomycetes. Although this kind of pathogenic bacteria is similar to fungi in morphology, it is closely related to diatoms and cyanobacteria in evolution, and belongs to the kingdom of anthracnose. Therefore, general fungal fungicides are often effective against Phytophthora soybean and Other oomycetes are invalid. The application of plant disease-resistant varieties has become an important means of achieving disease resistance in agricultural production due to overcoming the problems of phytotoxicity and enviro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/82
CPCC07K14/415C12N15/8282
Inventor 窦道龙景茂峰艾干白甜郑芷若
Owner NANJING AGRICULTURAL UNIVERSITY