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A kind of preparation method and application of bovine spinal cord peptide

A technology of bovine spinal cord and spinal cord, applied in the field of preparation of bovine spinal cord peptide, can solve the problems of not necessarily good curative effect and great harm to human body, etc.

Active Publication Date: 2021-07-20
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, radiation therapy and surgery are mainly used for syringomyelia, but radiation therapy is very harmful to the human body, and the curative effect is not necessarily good, and there are certain risks in surgical treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of bovine spinal cord peptide, comprising the following steps

[0033] (1) After the frozen bovine spinal cord is thawed at room temperature, it is broken into 3 cm spinal cord pieces and added with distilled water twice the weight and volume, that is, 100 grams of bovine spinal cord tissue is added with 200 ml of distilled water, and cooked at 100°C for 30 minutes.

[0034] (2) Tissue homogenization: Homogenize the above-mentioned cooked spinal cord tissue, add distilled water according to the mass volume ratio of spinal cord and distilled water at 2:1 during homogenization, that is, add 50 ml of distilled water to 100 grams of bovine spinal cord tissue, and the homogenization time is 10 minute.

[0035] (3) Degreasing: Use a degreasing agent to degrease the above-mentioned bovine spinal cord homogenate tissue to obtain a degreasing material. The degreasing agent is petroleum ether, and 200 milliliters of petroleum ether is added to the bovine spin...

Embodiment 2

[0041] (1) After the frozen bovine spinal cord is thawed at room temperature, it is broken into 3 cm spinal cord pieces and added with distilled water twice the weight and volume, that is, 100 grams of bovine spinal cord tissue is added with 200 ml of distilled water, and cooked at 100°C for 30 minutes.

[0042] (2) Tissue homogenization: Homogenize the above-mentioned cooked spinal cord tissue, add distilled water according to the mass volume ratio of spinal cord and distilled water at 2:1 during homogenization, that is, add 50 ml of distilled water to 100 grams of bovine spinal cord tissue, and the homogenization time is 10 minute.

[0043] (3) Degreasing: the above-mentioned bovine spinal cord homogenate tissue is degreased by a degreasing agent to obtain a degreasing material. The degreasing agent is petroleum ether, and 200 milliliters of petroleum ether is added to the bovine spinal cord homogenate tissue of every 100 grams, set every 4 hours Replace the degreasing agent...

Embodiment 3

[0049] (1) After thawing the frozen bovine spinal cord at room temperature, break it into 3 cm spinal cord pieces and add 2 times the mass volume of distilled water, that is, 100 grams of bovine spinal cord tissue plus 200 ml of distilled water, cook at 100 ° C for 30 minutes

[0050] (2) Tissue homogenization: Homogenize the above-mentioned cooked spinal cord tissue, add distilled water according to the mass volume ratio of spinal cord and distilled water at 2:1 during homogenization, that is, add 50 ml of distilled water to 100 grams of bovine spinal cord tissue, and the homogenization time is 10 minute

[0051] (3) Degreasing: the above-mentioned bovine spinal cord homogenate tissue is degreased by a degreasing agent to obtain a degreasing material. The degreasing agent is petroleum ether, and 200 milliliters of petroleum ether is added to the bovine spinal cord homogenate tissue of every 100 grams, set every 4 hours Replace the degreasing agent once, a total of 6 times, an...

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PUM

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Abstract

The invention discloses a preparation method of bovine spinal cord peptide, which comprises the following steps: thawing frozen bovine spinal cord at room temperature, cooking at high temperature, tissue homogenization, degreasing, enzymatic hydrolysis, ultrafiltration, freeze drying or spray drying to obtain bovine spinal cord peptide pink. The spinal cord is a part of the central nervous system of humans and vertebrates. The upper end is connected to the medulla oblongata, and paired nerves are sent out on both sides. There is an H-shaped gray matter area inside the spinal cord. The spinal cord is the pathway between the nerves and the brain, and it is also the lower level of many simple reflex activities. center. Therefore, the spinal cord is a very special tissue and organ of vertebrates. According to the theory of using viscera to replenish viscera in traditional Chinese medicine, the spinal cord must contain special functional factors. Analysis and detection showed that the fresh spinal cord contained nutrients, macro elements, trace elements, fatty acids and various amino acids needed by the human body. It can be seen that the spinal cord has a very high nutritional value, and the bovine spinal cord nutritional whole powder and bovine spinal cord polypeptide powder prepared by the present invention can be used to develop common food, health care products or in the field of medicine.

Description

technical field [0001] The invention relates to a preparation method and application of bovine spinal cord peptide, belonging to the field of biotechnology. It is a polypeptide prepared by hydrolysis using bovine spinal cord as a raw material, and has a good application prospect in the fields of food, health products and pharmaceuticals Background technique [0002] With the development of economic level, transportation industry and construction industry, the incidence of spinal cord injury is increasing year by year. Spinal cord injury is a serious trauma to the central nervous system, which can cause severe disability and partial neurological dysfunction, and spinal cord injury is the most serious complication of spinal cord injury, often leading to severe dysfunction of the limbs below the injured segment At present, the treatment for spinal cord injury mainly includes: physical therapy, surgical treatment and western medicine treatment. The effect of physical therapy i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/34A23L33/18A23L29/00A61K38/01A61P19/00
CPCA23V2002/00A61K38/00A23L29/045A23L33/18A61P19/00C07K2/00C12P21/06A23V2200/30A23V2250/55
Inventor 叶海青柳慧琴张铁华
Owner JILIN UNIV
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