Laser- and pressure-controlled perforation control method for solid-state nanopores

Abstract

The embodiment of the present application discloses a solid nanopore perforation control method based on laser and pressure modulation. One embodiment of the method includes: performing micro-nano preparation of a solid nanopore chip, performing underwater infiltration on the solid nanopore chip, building a nanopore chip test system with a confocal optical system, irradiating the surface of a solid membrane of the solid nanopore chip with laser, determining nanopore positions of the solid nanopore chip by ion current change, controlling the perforation of an object through changing control conditions, and when the nanopores are blocked, irradiating the nanopores through laser to decompose and remove pollutants. The method ensures the measurement signal-to-noise ratio and the capture rate,realizes reversible transition of the adjustment process and has a good application value. The method can effectively utilize the high-power laser irradiation to decompose and remove the surface pollutants of the nanopores, is simple and fast and ensures the long service life of the nanopore.

Description

technical field [0001] The invention relates to solid-state nanohole detection technology, in particular to a solid-state nanohole perforation control method based on laser and pressure modulation. Background technique [0002] Biomacromolecules are the main carrier of life activities. In the past few decades, the progress of biomacromolecules research methods and technologies has had an extremely significant impact on the development of life sciences. With the in-depth study of the life activity process, the detection and research of the structure and physical and chemical properties of biological macromolecules have begun to enter the single-molecule level. Compared with the traditional observation methods based on the overall average, single-molecule detection methods have the following advantages. First, through multiple continuous measurements, the inhomogeneous distribution of single-molecule properties can be displayed; second, by directly recording system disturbance...

AI Technical Summary

Problems solved by technology

As for the time resolution, the current perforation speed of the object to be measured is too fast, far exceeding the frequency response speed of the signal generator and the cut-off frequency of the filter, resulting in the inability to distinguish and identify its ultra-fine structure and become a solid-state nanopore. An important bottleneck for the further large-scale application of technology

Fologea and others systematically studied the relationship between the average perforation time of biomolecules and the experimental parameters of nanopores, and found that reducing the driving voltage, increasing the electrolyte concentration, increasing the viscosity coefficient, and lowering the temperature can slow down the perforation speed of biomolecules, but the deceleration effect Very limited (2-8x), at the cost of loss of SNR and capture rate

In addition, researchers use small-sized nanopores less than 5nm to decelerate through the strong interaction between biomolecules and pore walls. Bigger, i.e. introducing greater uncertainty to the perforation of biomolecules while slowing down

Therefore, there are few very effective methods for the time resolution and perforation process control of solid-state nanopore detection technology.

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