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Preparation method of astragalus polysaccharide lipidosome

A technology of astragalus polysaccharide and liposome, applied in the field of preparation of astragalus polysaccharide liposome

Inactive Publication Date: 2018-12-11
QIDONG CHUANGLU NEW MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the conventional dosage form of astragalus polysaccharide has defects in the process of use. For example, most of the drug has been degraded and metabolized before reaching the site of action, so large doses of continuous administration are required

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A preparation method of astragalus polysaccharide liposome, comprising the following steps: placing 8 parts of lecithin and 15 parts of cholesterol in a dry and clean eggplant-shaped bottle, adding 60 parts of absolute ethanol for ultrasonic dissolution, and depressurizing in a water bath at 45°C Remove ethanol by evaporation, so that the phospholipid film material forms a uniform lipid film on the wall of the eggplant-shaped bottle; dissolve 15 parts of APS in 70 parts of purified water to prepare the water phase, preheat it in a water bath at 45°C, and slowly instill it in the eggplant In the shaped bottle, oscillate while adding dropwise until the film is completely detached from the eggplant-shaped bottle wall and dissolved, and hydrated by rotation for 80 minutes; the resulting suspension is ultrasonically passed for 25 minutes, passed through a 0.45 μm microporous membrane for 3 times, and a 0.22 μm microporous filter. Membrane three times to obtain the astragalus ...

Embodiment 2

[0016] A preparation method of astragalus polysaccharide liposome, comprising the following steps: placing 7 parts of lecithin and 10 parts of cholesterol in a dry and clean eggplant-shaped bottle, adding 55 parts of absolute ethanol for ultrasonic dissolution, and depressurizing in a water bath at 40°C Remove ethanol by evaporation, so that the phospholipid film forms a uniform lipid film on the wall of the eggplant-shaped bottle; dissolve 10 parts of APS in 65 parts of purified water to prepare the water phase, preheat it in a water bath at 44°C, and slowly instill it in the eggplant In the bottle, vibrate while adding dropwise until the film is completely detached from the eggplant-shaped bottle wall and dissolved, then rotate and hydrate for 70 minutes; the resulting suspension is ultrasonicated for 20 minutes, passed through a 0.45 μm microporous membrane for 3 times, and a 0.22 μm microporous filter. Membrane three times to obtain the astragalus polysaccharide liposome su...

Embodiment 3

[0018] A preparation method of astragalus polysaccharide liposome, comprising the following steps: placing 9 parts of lecithin and 20 parts of cholesterol in a dry and clean eggplant-shaped bottle, adding 65 parts of absolute ethanol for ultrasonic dissolution, and depressurizing in a water bath at 50°C Remove ethanol by evaporation, so that the phospholipid film forms a uniform lipid film on the wall of the eggplant-shaped bottle; dissolve 20 parts of APS in 75 parts of purified water to prepare the water phase, preheat it in a water bath at 46°C, and slowly instill it in the eggplant Shake the bottle while adding it dropwise until the film completely falls off and dissolves from the wall of the eggplant-shaped bottle, then rotate and hydrate for 90 minutes; ultrasonically pass the resulting suspension for 30 minutes, pass through a 0.45 μm microporous membrane for 3 times, and then pass through a 0.22 μm microporous filter for 3 times. Membrane three times to obtain the astra...

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PUM

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Abstract

The invention discloses a preparation method for an astragalus polysaccharide lipidosome. The preparation method comprises the following steps that lecithin and cholesterol are placed in a dry and clean eggplant-shaped bottle, absolute ethyl alcohol is added for ultrasonic dissolution, and reduced pressure evaporation is carried out in a water bath at 40-50 DEG C so as to remove the ethanol, so that a phospholipid membrane material can form a uniform lipid membrane on the wall of the eggplant-shaped bottle; APS is dissolved in purified water so as to prepare an aqueous phase, preheating is carried out in the water bath at 44-46 DEG C, the aqueous phase is slowly dripped into the eggplant-shaped bottle, the eggplant-shaped bottle is shaken while the the aqueous phase is dripped until the membrane is completely stripped off and dissolved from the wall of the eggplant-shaped bottle, and rotary hydration is carried out for 70-90 min; and ultrasonic treatment is carried out on an obtained suspension for 20-30 minutes, the suspension passes through a microporous filter membrane so as to obtain an astragalus polysaccharide lipidosome suspension, and the astragalus polysaccharide lipidosome suspension is refrigerated at the temperature of 4 DEG C. The method has the advantages of being simple, convenient to use, rapid and easy to operate, and the prepared astragalus polysaccharide lipidosome is high in encapsulation efficiency, stable when stored at 4 DEG C and good in slow-release effect.

Description

technical field [0001] The invention relates to a preparation method of astragalus polysaccharide liposome. Background technique [0002] Astragalus is a commonly used bulk medicinal material, which was first recorded in "Shen Nong's Materia Medica". It has the effects of diuretic and swelling, invigorating qi and raising yang, promoting body fluid and nourishing blood, strengthening the surface and antiperspirant. Astragalus polysaccharide (APS) is the main active ingredient of Astragalus, which has a wide range of pharmacological activities, including immune regulation, anti-aging, anti-virus, anti-oxidation, anti-tumor, and anti-radiation effects. As a potential insulin sensitizer for the treatment of type 2 diabetes [0003] The current conventional dosage forms of astragalus polysaccharides have defects during use. For example, most of the drugs have been degraded and metabolized before reaching the site of action, so continuous administration of large doses is requir...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/715A61K47/28A61K47/24A61P3/10A61P37/02A61P39/06A61P35/00A61P39/00
CPCA61K9/127A61K31/715A61K47/24A61K47/28A61P3/10A61P35/00A61P37/02A61P39/00A61P39/06
Inventor 不公告发明人
Owner QIDONG CHUANGLU NEW MATERIAL CO LTD