Extraction and separation method and application of Polygonatum nonpolysaccharide components

A separation method and technology of Polygonatum chinensis are applied in the field of extraction and separation of polysaccharide non-polysaccharide components, which can solve the problems of incomplete separation, low extraction rate of effective components, limited application of Polygonatum chinensis, etc., and achieves simple preparation method, improved use value, and expanded application scope. Effect

Active Publication Date: 2021-11-05
HUNAN XINHUI PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polygonatum is extracted with ethanol multiple times in this application, and the concentration of adding ethanol is all constant every time, and the order of adding extractant in the extraction process is sherwood oil, ethyl acetate and n-butanol, and the polarity of extractant is from small to large, Using this extraction method will result in a low extraction rate of active ingredients in Rhizoma Polygonatum, and the separation between components is not complete, especially the polysaccharide components and non-polysaccharide components, which limits the application of Rhizoma Polygonatum

Method used

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  • Extraction and separation method and application of Polygonatum nonpolysaccharide components
  • Extraction and separation method and application of Polygonatum nonpolysaccharide components
  • Extraction and separation method and application of Polygonatum nonpolysaccharide components

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Experimental program
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Effect test

Embodiment 1

[0026] 1) Slicing the dried Rhizoma Polygonatum tuber into slices, adding ethanol water solution with a volume concentration of 95%, the mass ratio of ethanol water solution and Rhizoma Rhizoma Rhizoma Rhizoma Rhizoma Rhizoma Rhizoma Rhizome is 5:1, carries out the first reflux extraction at 80 ℃, after 2h, filters for the first time to obtain Add the ethanol aqueous solution of volume concentration 80% in the filter residue, the mass ratio of the 80% ethanol aqueous solution and the filter residue filtered for the first time is 5:1, carry out the second reflux extraction at 80 ℃, filter after 1h, combine twice The extracted filtrate is concentrated to obtain the crude extract of Rhizoma Polygonatum. The conditions for concentration are: the temperature is 50°C, the degree of vacuum is -0.09MPa, and the specific gravity of the concentrated solution is 1;

[0027] 2) Add 2 times the quality of distilled water to disperse the thick extract of Rhizoma Polygonatum, then extract wit...

Embodiment 2

[0029] 1) slice the dry Rhizoma Polygonatum tuber into slices, add 95% aqueous ethanol solution with volume concentration, the mass ratio of 95% aqueous ethanol solution and Rhizoma Polygonatum tuber is 6:1, carry out the first reflux extraction at 88°C, after 3h, the first Add the ethanol aqueous solution of volume concentration 80% to the filter residue obtained by the second filtration, the mass ratio of the 80% ethanol aqueous solution and the filter residue filtered for the first time is 5:1, carry out the second reflux extraction at 88 ° C, filter after 2h, The filtrates extracted twice were combined and concentrated to obtain the crude extract of Rhizoma Polygonatum. The conditions for concentration were: the temperature was 55°C, the degree of vacuum was -0.09MPa, and the specific gravity of the concentrated solution was 1.06;

[0030] 2) Add 2 times the quality of distilled water to disperse the thick extract of Rhizoma Polygonatum, then extract with sherwood oil, n-bu...

Embodiment 3

[0032] 1) Slice the dried Polygonatum tuber, add ethanol water solution with a volume concentration of 95%, the mass ratio of ethanol water solution and Polygonatum tuber is 5.5:1, carry out the first reflux extraction at 90°C, and filter for the first time after 2.5h Add an aqueous ethanol solution with a volume concentration of 80% to the obtained filter residue, the mass ratio of the 80% aqueous ethanol solution to the filter residue filtered for the first time is 4:1, carry out the second reflux extraction at 90°C, filter after 1.5h, and combine The filtrate extracted twice is concentrated to obtain the crude extract of Rhizoma Polygonatum. The conditions for concentration are: the temperature is 60°C, the degree of vacuum is -0.09MPa, and the specific gravity of the concentrated solution is 1.1;

[0033]2) Add 2 times the quality of distilled water to disperse the thick extract of Rhizoma Polygonatum, then extract with sherwood oil, n-butanol, ethyl acetate and dichloromet...

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Abstract

The invention discloses a method for extracting and separating non-polysaccharide components of Rhizoma Polygonatum. The steps are as follows: slice dried Rhizoma Rhizoma Polygonatum tubers, add ethanol with a concentration of 95%, perform the first reflux extraction, and add a concentration of 80% to the filter residue obtained through the first filtration. % of ethanol, carry out the second reflux extraction, filter, combine the filtrate extracted twice, and concentrate to obtain the thick extract of Rhizoma Polygonati; Extract with dichloromethane to obtain sherwood oil phase, n-butanol phase, ethyl acetate phase, dichloromethane phase respectively, dichloromethane phase and ethyl acetate phase are combined to obtain dichloromethane / ethyl acetate phase, and wherein The organic solvent is recovered to obtain concentrated extracts of three organic phases, and dried at 60°C to obtain the non-polysaccharide components of Rhizoma Polygonatum. The phase of dichloromethane / ethyl acetate has the best antitumor effect.

Description

technical field [0001] The invention belongs to the technical field of extracting methods of Polygonatum, and in particular relates to a method for extracting and separating non-polysaccharide components of Polygonatum and its application. Background technique [0002] Huang Jing (Polygonatum sibiricum), also known as: chicken head Polygonatum, yellow chicken dish, pen tube dish, claw ginseng, tiger ginger, chicken foot ginseng. It is a plant of the genus Polygonatum, with transverse rhizomes, cylindrical, enlarged nodules, whorled leaves, and sessile. Polygonatum is sweet in nature and tastes refreshing. It is a traditional Chinese herbal medicine with the same source of medicine and food. Its fleshy rhizome is thick and contains a lot of starch, sugar, fat, protein, carotene, vitamins and various other nutrients. It can be eaten raw or stewed Taking it can not only satisfy the hunger, but also have the function of fitness. It can double the strength, fill the muscles and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8969A61P35/00
CPCA61K36/8969A61K2236/333A61K2236/35A61K2236/39A61P35/00
Inventor 何述金王炜彭彩云周代俊周准
Owner HUNAN XINHUI PHARMA
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