Method for preparing grape seed proanthocyanidin extract

A proanthocyanidin and grape seed technology, applied in organic chemistry and other fields, can solve the problems of low utilization rate and waste of resources, and achieve the effect of easy operation and significant antioxidant activity

Inactive Publication Date: 2018-12-14
QIDONG CHUANGLU NEW MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most enterprises generally discard the skin seeds, use them as feed, burn materials or ferment them as fertilizers, the utilization rate is very low, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of grape seed proanthocyanidin extract, comprising the following steps: crush 20 parts of grape seeds, degrease with 75 parts of petroleum ether at 65°C for 6 hours, dry and place in a triangular flask, add 55 parts of pH4.0 buffer solution, adjust the activity of the cellulase solution to 100U / mg, enzymatically hydrolyze at 42°C for 2 hours, then rapidly raise the temperature to 95°C to fully inactivate, centrifuge at 2000r / min and save the supernatant, add 95 parts of ethanol solution with a volume fraction of 70% Put in a water bath at 55°C for 50 minutes, filter with suction, distill and concentrate under reduced pressure at 60°C until no droplets drip out, and freeze-dry the concentrate to obtain the crude extract of GSPE; add 15 parts of AB-8 macroporous resin to the Erlenmeyer flask, and then add 55 parts of 2mg / mL aqueous solution of GSPE crude extract were placed in a shaker at 28°C for 48 hours of vibration and adsorption at a shaking speed...

Embodiment 2

[0019] A preparation method of grape seed proanthocyanidin extract, comprising the following steps: crush 15 parts of grape seeds, degrease with 70 parts of petroleum ether at 60°C for 5 hours, dry and place in a triangular flask, add 50 parts of pH4.0 buffer solution, adjust the activity of the cellulase solution to 100U / mg, enzymatically hydrolyze at 40°C for 1.5h, then rapidly raise the temperature to 94°C to fully inactivate, centrifuge at 2000r / min and save the supernatant, add 90 parts of ethanol with a volume fraction of 65% The solution was placed in a water bath at 54°C for 45 minutes, filtered with suction, concentrated by distillation under reduced pressure at 55°C until no droplets dripped out, and the concentrate was freeze-dried to obtain the crude extract of GSPE; add 10 parts of AB-8 macroporous resin to the conical flask, and then Add 50 parts of GSPE crude extract aqueous solution with a concentration of 2mg / mL, put it into a shaker at 25°C for 47 hours of vib...

Embodiment 3

[0021] A preparation method of grape seed proanthocyanidin extract, comprising the following steps: crush 25 parts of grape seeds, degrease with 80 parts of petroleum ether at 70°C for 7 hours, dry and place in a conical flask, add 60 parts of pH4.0 buffer solution, adjust the activity of the cellulase solution to 100U / mg, enzymatically hydrolyze at 44°C for 2.5h, then rapidly raise the temperature to 96°C to fully inactivate, centrifuge at 2000r / min and save the supernatant, add 100 parts of ethanol with a volume fraction of 75% The solution was placed in a water bath at 56°C for 55 minutes, filtered with suction, concentrated by distillation under reduced pressure at 65°C until no droplets dripped out, and the concentrate was freeze-dried to obtain the crude extract of GSPE; add 20 parts of AB-8 macroporous resin to the conical flask, and then Add 60 parts of GSPE crude extract aqueous solution with a concentration of 2mg / mL, put it into a shaker at 30°C for 49 hours of vibra...

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PUM

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Abstract

The invention discloses a method for preparing grape seed proanthocyanidin extract. The method comprises the following steps: grinding grape seeds, degreasing with petroleum ether, drying, adding intoa triangular flask, adding a buffer solution, performing enzymolysis for 1.5-2.5 hours, rapidly raising the temperature, fully inactivating, performing centrifugal separation, preserving the supernatant, adding an ethanol solution having the volume fraction of 65-75% into a water bath for 45-55 minutes, performing suction filtration, performing reduced pressure distillation, and performing freezedrying on the concentrate so as to obtain the crude GSPE; adding an AB-8 macroporous resin into a conical flask, adding an aqueous solution of the crude GSPE, performing oscillating adsorption into atable for 47-49 hours, filtering, pouring the resin washed with distilled water into ethanol having the volume fraction of 50-60% for desorbing for 25-27 hours, filtering the desorbed solution, performing reduced pressure distillation, and performing freeze drying, thereby obtaining the product. The method is simple, convenient and rapid, a large quantity of grape seed proanthocyanidin extract issuccessfully prepared, and the extract has obvious antioxidant activity and can be used for drug preparation.

Description

technical field [0001] The invention relates to a preparation method of grape seed proanthocyanidin extract. Background technique [0002] my country is rich in grape resources, with an annual output of nearly 6 million tons. About 80% of these grapes are used for wine making, 13% are eaten as fresh fruit, and 7% are used for processing fruit juice and other grape products. The production of industrial wine is accompanied by a large amount of waste. The world produces tens of thousands of tons of wine grape waste every year, mainly including grape skins and grape seeds. As the wine market continues to grow, the resulting by-products of winemaking have gradually become an environmental problem that cannot be ignored. Most enterprises generally discard the skin seeds, use them as feed, burn materials or ferment them as fertilizers, and the utilization rate is very low, resulting in a great waste of resources. [0003] Studies in recent years have found that grape seeds have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 不公告发明人
Owner QIDONG CHUANGLU NEW MATERIAL CO LTD
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