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A kind of synergist of food biological preservative and using method thereof

A technology of biological preservatives and synergists, applied in food ingredients as antimicrobial preservation, food science, food ingredients, etc., can solve the problem of few biofilm degrading enzymes and no reports on biofilm degrading enzymes and food biopreservatives relationship and other issues, to achieve the effect of improving biofilm degradation, inhibiting quorum sensing information exchange, and precise action sites

Active Publication Date: 2021-12-28
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on biofilm-degrading enzymes, and there is no report on the relationship between biofilm-degrading enzymes and food biopreservatives

Method used

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  • A kind of synergist of food biological preservative and using method thereof
  • A kind of synergist of food biological preservative and using method thereof
  • A kind of synergist of food biological preservative and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The product of the present invention is composed of the following raw materials in weight percentage: 20% of cellulase, 20% of lipase, 30% of protease, 12% of tea polyphenols, and 18% of cinnamon extract. The specific usage method is as follows:

[0023] (1) Weigh the raw materials according to the weight percentage, mix them uniformly to obtain a powdery mixture, put it into a clean food packaging container, seal it, and store it away from light;

[0024] (2) Take a certain weight of the mixed powder obtained in step (1), mix and dissolve it with chitosan at a weight ratio of 1:25.

[0025] (3) if figure 1 Shown, compared with using chitosan alone, the inhibitory rate of product of the present invention to chitosan inhibits Pseudomonas, Shewanella and Vibrio parahaemolyticus has improved respectively 60%, 15.9% and 186.7%, has Obvious synergy.

Embodiment 2

[0027] The product of the present invention is composed of the following raw materials in weight percentage: 20% of cellulase, 20% of lipase, 30% of protease, 12% of tea polyphenols, and 18% of cinnamon extract. The specific usage method is as follows:

[0028] (1) Weigh the raw materials according to the weight percentage, mix them uniformly to obtain a powdery mixture, put it into a clean food packaging container, seal it, and store it away from light;

[0029] (2) Take a certain weight of the mixed powder obtained in step (1), mix and dissolve it with polylysine at a weight ratio of 1:20.

[0030] (3) if figure 2 As shown, compared with using polylysine alone, the inhibitory rate of the product of the present invention to polylysine to inhibit Pseudomonas, Shewanella and Vibrio parahaemolyticus has increased by 180.5%, 108.3% and 91.7% respectively , with obvious synergistic effect.

Embodiment 3

[0032] The product of the present invention is composed of the following raw materials in weight percentage: 20% of cellulase, 20% of lipase, 30% of protease, 12% of tea polyphenols, and 18% of cinnamon extract. The specific usage method is as follows:

[0033] (1) Weigh the raw materials according to the weight percentage, mix them uniformly to obtain a powdery mixture, put it into a clean food packaging container, seal it, and store it away from light;

[0034] (2) Take a certain weight of the mixed powder obtained in step (1), mix and dissolve it with L-phenyllactic acid at a weight ratio of 1:25.

[0035] (3) if image 3 As shown, compared with using L-phenyllactic acid alone, the inhibition rate of the product of the present invention to L-phenyllactic acid inhibiting Pseudomonas, Shewanella and Vibrio parahaemolyticus has increased by 260.0%, 123.1% and 40.3% respectively , with obvious synergistic effect.

[0036] (4) Compared with using L-phenyllactic acid alone, the...

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Abstract

A synergist for food biological preservatives and its use method, which is composed of the following raw materials in weight percentage: 15-20% cellulase, 15-20% lipase, 30-40% protease, 10-20 tea polyphenol %, cinnamon extract 10‑20%. The method of use is to weigh the raw materials in proportion, mix them evenly; mix and dissolve them with food biological preservatives in a weight ratio of 1:20‑30. The present invention starts from the new targets of spoilage bacteria biofilm control and quorum sensing inhibition for the first time, and uses biofilm degrading enzymes and quorum sensing inhibitors in combination to interfere with the formation of spoilage bacteria biofilms and reduce the thickness of spoilage bacteria biofilms. Inhibit the exchange of quorum sensing information between spoilage bacteria, thereby increasing the sensitivity of spoilage bacteria to biological preservatives and enhancing the effect of biological preservatives. The action target of this synergist is the biofilm and quorum sensing system of spoilage bacteria. The action site is precise, and it is not a lethal effect in a simple sense. The spoilage bacteria do not produce resistance, which has incomparable advantages in the existing technology.

Description

technical field [0001] The invention belongs to the field of food preservation and food additives, and in particular relates to a synergist for food biological preservatives and a using method thereof. Background technique [0002] The toxic and side effects of traditional chemical preservatives such as sodium benzoate, sodium nitrite, and sodium diacetate have attracted increasing attention. Therefore, finding safe and non-toxic biological preservatives has become a hot spot of concern. The biological preservatives currently used in food mainly include chitosan, tea polyphenols, nisin, bamboo leaf antioxidants, rosemary extract, phytic acid, etc. At present, the biggest disadvantage of biological preservatives is the high cost, and the antiseptic effect is generally lower than that of chemical preservatives, which limits the use and promotion of biological preservatives. [0003] Bacterial quorum sensing system and bacterial biofilm are new targets for bacterial control i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L3/3571A23L3/3472
CPCA23L3/3472A23L3/3571A23V2002/00A23V2200/10A23V2250/21A23V2250/2132A23V2250/214
Inventor 刘尊英董汝月曾名湧
Owner OCEAN UNIV OF CHINA
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