Measurement method of volatile fatty acid in tobacco leaf

A volatile fatty acid and determination method technology, applied in the field of tobacco chemical component analysis, can solve the problems of volatile component loss and inability to measure volatile fatty acid, etc., to reduce the loss of volatile components, the detection process is simple and convenient, and the analysis and determination sensitivity is high Effect

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for the determination of volatile fatty acids in tobacco leaves, so as to solve the problem that the ex

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  • Measurement method of volatile fatty acid in tobacco leaf
  • Measurement method of volatile fatty acid in tobacco leaf
  • Measurement method of volatile fatty acid in tobacco leaf

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Embodiment 1

[0033] The assay method embodiment 1 of volatile fatty acid in tobacco leaf of the present invention, chooses flue-cured tobacco C3F sample, adopts the following steps:

[0034] 1) Crush the tobacco leaves and pass through a 40-mesh sieve, weigh 0.5g of tobacco leaf powder and add 3mL of CH 2 Cl 2 As an extraction solvent, 50 μL of trans-2-hexenoic acid internal standard solution with a concentration of 20 μg / mL was added, vortexed at 2500 rpm for 30 min, and then filtered with a 0.45 μm filter membrane to obtain an extract.

[0035] 2) Take 1.0 mL of the extract in a chromatographic bottle, add 200 μL of derivatization reagent (N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)) and shake evenly, and derivatize in a water bath at 60°C for 30 minutes. After cooling to room temperature, it was directly detected by GC-MS / MS.

[0036] GC-MS / MS analysis conditions are as follows: DB-5MS (60m×0.25mm×0.25μm) capillary column separation, inlet temperature: 280°C, injection volume 1μ...

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Abstract

The invention relates to a measurement method of volatile fatty acid in tobacco leaf. The measurement method comprises the following steps: 1) adding dichloromethane in a tobacco leaf sample to perform extraction, separating to obtain extract liquor; and 2) adding derivatization reagent in the extract liquor, performing gas chromatograph-tandem mass spectrometer after the derivatization reaction.The measurement method of the volatile fatty acid in the tobacco leaf provided by the invention, the tobacco leaf sample is extracted by using the dichloromethane at the normal temperature, the C1-C10volatile fatty acid can be effectively extracted, and the detection analysis is performed after the derivatization reaction; the whole measurement process is less in step and compact in flow, the loss of the volatile component is reduced, the measurement of formic acid, acetic acid, butyric acid, 2-methylbutyric acid, 2-butenoic acid, isovaleric acid, valeric acid, senecioic acid, tiglic acid, 3-methylvaleric acid, 4-methylvaleric acid and like seventeen volatile fatty acids; a measurement result on the volatile fatty acid in the tobacco leaf is more comprehensive and accurate.

Description

technical field [0001] The invention belongs to the field of tobacco chemical component analysis, and in particular relates to a method for measuring volatile fatty acids in tobacco leaves. Background technique [0002] Tobacco leaves contain a large amount of fatty acids, accounting for about 10% of their dry weight. Fatty acid has a unique aroma and acidity, which is not only an important part of the aroma quality and style characteristics of cigarettes, but also can change the acid-base balance of smoke, and then have a significant impact on the strength, irritation and taste of cigarettes. According to the strength of volatility, fatty acids can be divided into volatile, semi-volatile and non-volatile fatty acids. Usually, volatile fatty acids refer to lower fatty acids below C10, which account for about 0.1%-0.2% of the dry weight of tobacco leaves . [0003] Volatile fatty acids have a great impact on the sensory quality of tobacco, for example: C1-C3 fatty acids hav...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 薛聪王冰余晶晶张晓兵蔡君兰赵晓东刘克建秦亚琼刘绍锋刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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