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An immunochromatographic test paper for detecting pyrethroids

An immunochromatographic test paper and pyrethroid technology, which is applied in the directions of measuring devices, analytical materials, instruments, etc., can solve the problem of high technical requirements for pretreatment and operators, poor repeatability and reproducibility of analytical results, and expensive instruments and equipment, etc. problems, to achieve the effect of protecting the health of consumers, obvious economic and social benefits, and showing the image of the results

Inactive Publication Date: 2021-01-01
ZHENGZHOU INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of the biological identification method is that the sample to be tested does not need to be very pure, but the disadvantage is that the sensitivity is low and the experimental period is long
The advantage of chemical analysis is that it is economical and practical, but it cannot be accurately quantified, and the repeatability and reproducibility of the analysis results are poor.
The instrumental analysis method has the advantages of high separation, high detection efficiency and rapid analysis ability, but it has high technical requirements for sample pretreatment and operators, and the equipment is expensive, so it is not suitable for on-site rapid detection

Method used

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  • An immunochromatographic test paper for detecting pyrethroids
  • An immunochromatographic test paper for detecting pyrethroids
  • An immunochromatographic test paper for detecting pyrethroids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The immunochromatographic test paper for detecting pyrethroids of the present invention, with reference to Figure 1-2 , including a support and an adsorption layer fixed on the support, the adsorption layer is a sample pad 1, a binding pad 2, a chromatographic membrane 3 and an absorption pad 4 in order from the test end, and it is characterized in that the binding pad is adsorbed with Nanomaterial-labeled anti-PBA monoclonal antibody PBA-mAb; the chromatographic membrane is provided with a stealth detection blot 5 printed with a carrier protein (PBA artificial antigen) solution coupled to PBA and a goat anti- or rabbit anti-mouse Stealth control blot 6 printed by IgG antibody solution; the nanomaterials are nitrogen-doped carbon nanomaterials, carbon nanomaterials, and carbon quantum dot fluorescent nanoparticles.

[0046] The support body includes a bottom layer 7 arranged on the bottom surface of the adsorption layer and a surface layer 8 arranged on the top surface...

Embodiment 2

[0057] This embodiment detects pyrethroid test paper, mainly including: the preparation of PBA artificial antigen, PBA monoclonal antibody (PBA-mAb), the preparation of nitrogen-doped carbon nanomaterials (N-CDs) labeled PBA antibody and based on N-CDs labeling The steps such as the preparation of immunochromatographic test paper, wherein, the preparation method of each product is as follows:

[0058] 1. Preparation of PBA artificial antigen (PBA-BSA)

[0059] 10 mg of bovine serum albumin (BSA) was weighed and placed in a 10 mL screw bottle, and pH 9.0 carbonate buffer solution was used to prepare 10% BSA activation solution A with a mass fraction. Weigh 1mg of fenpropathrin (PBA) and add 2mL of 0.1mol / L NaIO 4 Dissolve to make Solution B. Add solution A to solution B and let it react at room temperature for 30min, then add 0.1mL NaBH 4 The buffer was stirred and mixed, adjusted to pH 9.0 with 1mol / L NaOH, reacted at 4°C overnight, dialyzed with 0.01mol / L pH 7.4 phosphate ...

Embodiment 3

[0084] The detection of pyrethroid test paper in this embodiment mainly includes: preparation of PBA artificial antigen, PBA monoclonal antibody (PBA-mAb), preparation of carbon nanomaterial-labeled PBA antibody, and preparation of immunochromatographic test paper based on carbon nanomaterial labeling, etc. Step, wherein, the preparation method of each product is as follows:

[0085] 1. Preparation of PBA artificial antigen (PBA-BSA)

[0086] With embodiment 2.

[0087] 2. Preparation of PBA-mAb

[0088] With embodiment 2.

[0089] 3. Preparation of immunochromatographic test paper based on carbon nanomaterial labeling

[0090] (1) Preparation of carbon nanomaterial TPCA

[0091] Dissolve 1.5g citric acid and 1.62g cysteamine hydrochloride in 7.5mL ultrapure water, transfer the solution to a 50mL polytetrafluoroethylene liner after fully dissolving, and then place the liner in a high-pressure reactor. React at 200°C for 3 hours. After the reaction, the product was suction...

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Abstract

The invention discloses immunochromatographic test paper for detecting pyrethroid. The test paper comprises a support body and an adsorption layer fixed on the support body; the adsorption layer comprises a sample pad, a bonding pad, a chromatographic membrane and an absorption pad sequentially from a test end; an anti-PBA monoclonal antibody labeled by a nano-material is adsorbed on the bonding pad; an invisible detection print printed by a PBA artificial antigen solution and an invisible contrast print printed by a goat anti- or rabbit anti-mouse IgG antibody solution are arranged on the chromatographic membrane; and the nano-material is a nitrogen-doped carbon nano-material, a carbon nano-material or carbon quantum dot fluorescent nano-particles. A test strip has the characteristics ofbeing high in specificity, high in sensitivity, high in stability, good in safety, simple and convenient, quick, visual in result display, intuitive, wide in application range, convenient to carry andquantifiable. The test paper has extremely important significance in the aspects of guaranteeing food safety and protecting health of consumers, thereby having obvious economic and social benefits.

Description

technical field [0001] The invention relates to an immunochromatographic test paper, in particular to an immunochromatographic test paper for detecting pyrethroids (PBA). Background technique [0002] Pyrethroids (Pyrethroids) are developed on the basis of the chemical structure of natural pyrethroids, and are widely used in the field of agricultural pests, sanitation pest control and grain storage. At present, together with organophosphorus and carbamate pesticides, they are called the three most widely used pesticides, and the scope of use is second only to organophosphorus insecticides. However, it has a long residual period and is highly toxic to some non-target organisms; after entering the body through the enrichment of the food chain, it can cause dizziness, headache, nausea and skin irritation adverse reactions, and has a negative effect on mammalian reproductive, immune and Cardiovascular toxic side effects, the Food and Agriculture Organization of the United Natio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/577
Inventor 职爱民谢光辉丁勇贾国超孙勇赵国欣程丽英王芳孙浩冉李靖靖
Owner ZHENGZHOU INST OF TECH