Culture method of lactic acid bacteria for feed

A culture method and technology of lactic acid bacteria, applied in the field of microorganisms, to achieve the effects of improving acid stress resistance and reproduction number, convenient operation and good culture effect

Inactive Publication Date: 2018-12-25
黄拥亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The high-density culture of lactic acid bacteria has been widely used in the dairy industry, while the research

Method used

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  • Culture method of lactic acid bacteria for feed
  • Culture method of lactic acid bacteria for feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] For the cultivation method of lactic acid bacteria for feed, the inoculation amount of the seed liquid is 1.8v / v%. The activated Lactobacillus seed liquid for feed is inoculated into the fermentation medium containing whey protein hydrolyzate. Fermenting and culturing at 100 rpm for 14 hours to obtain a lactobacillus fermentation liquid for feeding; wherein the whey protein hydrolyzate is obtained by acid hydrolyzing whey protein added with inulin in hydrochloric acid solution containing D-configuration acid.

[0038] The above fermentation medium contains whey protein hydrolyzate 20g / L, yeast powder 2g / L, glucose 14g / L, buffer salt 5g / L, Mg 2+ Water-soluble compound 0.3g / L, Fe 2+ Water-soluble compound 0.1g / L, MnSO 4 0.04g / L; Mg 2+ The water soluble compound is MgSO 4 ; 2+ The water soluble compound is FeSO 4 ; Wherein; Buffer salt is sodium citrate.

[0039] The above inulin has a degree of polymerization of 15; the inulin contains 2.7% of gamma-inulin.

[0040...

Embodiment 2

[0042] For the cultivation method of lactic acid bacteria for feed, the inoculum amount of the seed liquid is 2.05v / v%. The activated Lactobacillus seed liquid for feed is inoculated into the fermentation medium containing whey protein hydrolyzate. Fermentation was carried out at 110rpm for 15 hours to obtain a lactobacillus fermentation liquid for feeding; wherein the whey protein hydrolyzate was obtained by acid hydrolyzing whey protein added with inulin in a hydrochloric acid solution containing D-configuration acid.

[0043] The above fermentation medium contains whey protein hydrolyzate 26g / L, yeast powder 4g / L, glucose 20g / L, buffer salt 7.5g / L, Mg 2+ Water-soluble compound 0.4g / L, Fe 2+Water-soluble compound 0.2g / L, MnSO 4 0.05g / L; Mg 2+ The water soluble compound is MgCl 2 ; 2+ The water soluble compound is FeCl 2 ; Wherein the buffer salt is potassium dihydrogen phosphate, sodium citrate and sodium acetate in a weight ratio of 1:0.8:0.4.

[0044] The degree of p...

Embodiment 3

[0047] For the cultivation method of lactic acid bacteria for feed, the inoculation amount of the seed liquid is 2.3v / v%. The activated Lactobacillus seed liquid for feed is inoculated into the fermentation medium containing whey protein hydrolyzate. The fermented culture was carried out at 120rpm for 16 hours to obtain a lactobacillus fermentation liquid for feeding; wherein the whey protein hydrolyzate was obtained by hydrolyzing the whey protein added with inulin with hydrochloric acid solution containing D-configuration acid.

[0048] The above fermentation medium contains whey protein hydrolyzate 32g / L, yeast powder 6g / L, glucose 25g / L, buffer salt 10g / L, Mg 2+ Water-soluble compound 0.5g / L, Fe 2+ Water-soluble compound 0.3g / L, MnSO 4 0.06g / L; Mg 2+ The water soluble compound is MgCO 3 ; 2+ The water soluble compound is (NH 4 ) 2 Fe(SO 4 ) 2 ; Wherein the buffer salt is calcium carbonate.

[0049] The above-mentioned inulin has a degree of polymerization of 18; t...

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Abstract

The invention provides a culture method of lactic acid bacteria for feed and belongs to the technical field of microorganisms. The culture method includes: inoculating an activated lactobacillus seedsolution for feed to a fermentation culture medium with the pH being 6.3-6.7 and containing whey protein hydrolysate to perform shake culture so as to obtain the lactobacillus fermentation broth for feed, wherein the whey protein hydrolysate is obtained by hydrolyzing whey protein with a hydrochloric acid solution containing D-configuration acid. The culture method has the advantages that high-density culture can be achieved, and the used fermentation culture medium can prevent oxygen from damaging the lactic acid bacteria for feed, provide more ATP for the lactic acid bacteria for feed, satisfy the growth needs of the lactic acid bacteria for feed for a long time, and increase the acid stress resistance and reproduction number of the lactic acid bacteria for feed.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for cultivating lactic acid bacteria for feeding. Background technique [0002] Entering the new century, my country's livestock and poultry breeding industry has developed rapidly. The process of large-scale farming is getting faster and faster, and scientific farming has also been widely used. However, the disadvantages that have always existed in the breeding industry have not been well resolved. For example, drug residues and bacterial drug resistance caused by long-term abuse of antibiotics are potentially harmful to human health. Furthermore, if the feces and sewage produced in livestock and poultry breeding are not properly treated or utilized, they will seriously pollute the environment and affect human survival. The introduction and in-depth study of the theory of microecology is the main means to solve the problems in the breeding industry, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20
Inventor 黄拥亮
Owner 黄拥亮
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