Preparation method of flexible metallic oxide nanofiber phosphorylated peptide enrichment material

A technology of nanofibers and phosphorylated peptides, which is applied in the direction of artificial filaments made of inorganic materials, can solve the problems of low metal oxide content on the fiber surface, poor enrichment capacity of phosphorylated peptides in composite fibers, and easy fracture of nanofiber membranes. Efficient and effective enrichment and purification, effective enrichment and purification, good continuity
CN109095894AInactive Publication Date: 2018-12-28XI'AN POLYTECHNIC UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XI'AN POLYTECHNIC UNIVERSITY
Publication Date
2018-12-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a flexible metallic oxide nanofiber phosphorylated peptide enrichment material. The preparation method of the flexible metallic oxide nanofiber phosphorylated peptide enrichment material includes the steps that 1, metal salt is added into corresponding solvent, stirring is conducted to generate metal ions, then, a chelating agent is added, stirring is conducted again, and a precursor solution is obtained, wherein the ratio of the metal salt to the solvent is 1 g:(10-80) ml, and the molar ratio of the metal salt to the chelating agent is 1:(0.01-0.4); 2, the obtained precursor solution is subjected to electrostatic spinning to obtain a precursor nanofiber membrane; 3, the precursor nanofiber membrane is calcined in air, the calcining temperature gradually increases to 500-1200 DEG C from room temperature, calcining is kept for 30-120 min at the highest calcining temperature, and then the flexible metallic oxide nanofiber membrane is obtained. The preparation method of the flexible metallic oxide nanofiber phosphorylated peptide enrichment material is simple in process, and phosphorylated protein and phosphorylated peptide fragments canbe effectively enriched and purified efficiently and repeatedly.
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Description

technical field

[0001] The invention belongs to the technical field of new materials, and relates to a preparation method of a flexible metal oxide nanofiber phosphorylated peptide enrichment material. Background technique

[0002] With the deepening of life science research, phosphoproteomics has become a research hotspot. As an important means of post-translational protein modification, protein phosphorylation regulates the whole process of human life activities, including signal transduction, cell proliferation and cytoskeleton regulation. The research method of protein phosphorylation is to use biological mass spectrometry to identify phosphorylated peptides on the basis of protein enzymatic hydrolysis. However, the phosphorylated peptides show low abundance in the enzymatic hydrolysis products, and the presence of high-abundance non-phosphorylated peptides will inhibit the detection of mass spectrometry signals, and the content of phosphorylated proteins is low, so mas...

Claims

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