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Silk fibroin three-dimensional porous scaffold and preparation method thereof

A three-dimensional porous, silk fibroin technology, applied in medical science, prosthesis, etc., can solve the problems of inability to transport and absorb nutrients, poor water absorption and water retention performance of gel film, poor processability, etc., and achieve good biocompatibility. high water absorption, excellent mechanical properties

Active Publication Date: 2018-12-28
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difference with the present invention is: based on this preparation method, silk fibroin gel can only form a two-dimensional dense membrane material, has poor processability, and is difficult to form a strong silk fibroin hydrogel with a three-dimensional network structure, and can be used as a biomedical material. Restricted; Secondly, the gel component described in the present invention is different from the component of the gel described in Publication No. CN106310349A, polysaccharide is added in the silk fibroin gel of the present invention; Again, compared with the present invention, the gel The water absorption and water retention performance of the film is poor, and it is a dense film material, which cannot transport and absorb nutrients

Method used

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  • Silk fibroin three-dimensional porous scaffold and preparation method thereof
  • Silk fibroin three-dimensional porous scaffold and preparation method thereof
  • Silk fibroin three-dimensional porous scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) degumming: the silk fiber is placed in the Na that mass fraction is 0.05wt% 2 CO 3 Boil in an aqueous solution for 30 minutes, wash thoroughly with deionized water, repeat the above process three times, dry in an oven at 60°C for 24 hours, and loosen for later use;

[0035] (2) Melting silk: Weigh and measure CaCl respectively 2 and formic acid (purity greater than 98%), the preparation of salt concentration is 30g / L of CaCl 2 -Formic acid solution, weigh 3g silk fibroin and add 100mL CaCl 2 - Manually shake in the formic acid solution for 10-30 minutes, and let stand at room temperature for 1 hour to obtain the silk fibroin solution;

[0036] (3) Dissolving sugar: Weigh 2 g of hyaluronic acid and add it to the silk fibroin solution, shake it manually, and let it stand at room temperature for 3 hours to obtain a silk fibroin polysaccharide blend solution;

[0037] (4) Preparation of silk fibroin hydrogel: inject the above-mentioned silk fibroin proteoglycan blen...

Embodiment 2

[0040] (1) degumming: the silk fiber is placed in the Na that mass fraction is 0.05wt% 2 CO 3 Boil in an aqueous solution for 30 minutes, wash thoroughly with deionized water, repeat the above process three times, dry in an oven at 60°C for 24 hours, and loosen for later use;

[0041] (2) Melting silk: Weigh and measure CaCl respectively 2 and formic acid (purity greater than 98%), the preparation of salt concentration is 100g / L of CaCl 2 -Formic acid solution, weigh 6g silk fibroin and add 100mL CaCl 2 - Manually shake in the formic acid solution for 10-30 minutes, and let stand at room temperature for 1 hour to obtain the silk fibroin solution;

[0042] (3) Dissolving sugar: Weigh 5g of hyaluronic acid and add it to the silk fibroin solution, shake it manually, and let it stand at room temperature for 3 hours to obtain a silk fibroin polysaccharide blend solution;

[0043] (4) Preparation of silk fibroin hydrogel: inject the above-mentioned silk fibroin proteoglycan blen...

Embodiment 3

[0046] (1) degumming: the silk fiber is placed in the Na that mass fraction is 0.05wt% 2 CO 3 Boil in an aqueous solution for 30 minutes, wash thoroughly with deionized water, repeat the above process three times, dry in an oven at 60°C for 24 hours, and loosen for later use;

[0047] (2) Melting silk: Weigh and measure CaCl respectively 2 and formic acid (purity greater than 98%), the preparation of salt concentration is 50g / L of CaCl 2 -Formic acid solution, weigh 10g silk fibroin and add 100mL CaCl 2 - Manually shake in the formic acid solution for 10-30 minutes, and let stand at room temperature for 1 hour to obtain the silk fibroin solution;

[0048] (3) Dissolving sugar: Weigh 5g of hyaluronic acid and add it to the silk fibroin solution, shake it manually, and let it stand at room temperature for 3 hours to obtain a silk fibroin polysaccharide blend solution;

[0049] (4) Preparation of silk fibroin hydrogel: inject the above-mentioned silk fibroin proteoglycan blen...

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Abstract

The invention relates to a preparation method for a silk fibroin three-dimensional porous scaffold and the silk fibroin three-dimensional porous scaffold prepared from the method. The preparation method comprises the following steps of preparing silk fibroin by degumming silk fibers; and dissolving the silk fibroin in a salt-acid binary solution and standing to obtain a silk fibroin solution, adding polysaccharides for dissolution and standing to obtain a silk fibroin and polysaccharide blending solution; preparing a silk fibroin hydrogel by dialysis of the blending solution; and freeze-dryingthe hydrogel to obtain the silk fibroin three-dimensional porous scaffold. The method is simple to operate, a process is short, the pore diameter of the prepared scaffold is adjustable, the scaffoldhas good mechanical properties and hydroscopicity, and a series of alternative materials can be provided for the field of biomedical materials.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a silk fibroin three-dimensional porous scaffold and a preparation method thereof. Background technique [0002] At present, hydrogel has become an important carrier in the fields of biomaterials, biomedicine and structural biology. Traditionally, although double-network hydrogels, nanocomposite hydrogels, and topological hydrogels have excellent mechanical properties, most of them require complex synthesis, assembly, and cross-linking processes, which are long and unavoidable. Use of toxic solvents and reduction in overall performance. For example, most synthetic hydrogels have poor biocompatibility, and their degradation products are potentially toxic during the degradation process, which restricts the application of most synthetic hydrogels in the field of biomedicine. [0003] Silk fibroin protein is an important resource with Chinese characteristics and advantages. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00C08L5/08A61L27/56A61L27/52A61L27/50A61L27/22A61L27/20
CPCA61L27/20A61L27/227A61L27/50A61L27/52A61L27/56C08J3/075C08J2389/00C08J2405/08C08L89/00C08L5/08
Inventor 张强韩国聪闫书芹尤仁传李秀芳罗祖维
Owner WUHAN TEXTILE UNIV
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