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Grafting method for irradiating actinidia chinensis branches by means of high-energy carbon ion beam

A kiwifruit and ion beam technology, applied in grafting, botanical equipment and methods, applications, etc., can solve problems such as easy biological damage, and achieve the effect of improving the survival rate of grafting

Inactive Publication Date: 2019-01-01
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial hormones (colchicine doubling) and irradiation (60Co-γ) help increase mutation frequency, but are prone to biological damage

Method used

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  • Grafting method for irradiating actinidia chinensis branches by means of high-energy carbon ion beam
  • Grafting method for irradiating actinidia chinensis branches by means of high-energy carbon ion beam

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select the annual branches of Chuhong kiwifruit with full buds, cut the branches into 6cm long sections, each section contains a full bud, and leave about 1cm of branches on the upper part of the bud.

[0025] Put the buds of the cut branches facing upwards and place them side by side in double layers. The buds do not block each other. The diameter of each group of buds is 3.5cm, such as figure 1 As shown, use double-sided tape to fix the back of the bud body on the operation board. Carry out high-energy carbon ion beam irradiation, and the radiation dose of ion beam treatment seeds is 10Gy.

[0026] The branches treated with high-energy carbon ion beams were wrapped with wet newspaper and stored in a refrigerator at 4°C until use.

[0027] Select a strong biennial wild kiwifruit rootstock, cut it with branch shears at a distance of 80 cm from the ground, and cut obliquely 1.5 to 2 cm toward the cambium at about 5 mm on the east and west sides of the main section. The...

Embodiment 2

[0031] Select the annual Chuhong kiwifruit branches with full buds, cut the branches into 5cm long sections, each section contains a full bud, and leave about 1cm of branches on the upper part of the bud.

[0032] Put the buds of the cut branches upwards and place them side by side. Use double-sided tape to fix the back of the buds on the operation panel, and then irradiate them with high-energy carbon ion beams. The radiation dose of seeds treated with high-energy carbon ion beams is 20Gy.

[0033] The branches treated with high-energy carbon ion beams were wrapped with wet newspaper and stored in a refrigerator at 4°C until use.

[0034] Select a strong biennial wild kiwi rootstock, cut it with branch shears at a distance of 85 cm from the ground, and cut obliquely 1.5 to 2 cm toward the cambium at about 5 mm on the east and west sides of the main section. The lower end of the scion irradiated by the high-energy carbon ion beam is cut into a horseshoe-shaped slope to expose ...

Embodiment 3

[0038] Select the annual Chuhong kiwifruit branches with full buds, cut the branches into 5.5cm long sections, each section contains a full bud, and leave about 1cm of branches on the upper part of the bud.

[0039] Place the buds of the cut branches upwards and place them side by side. Use double-sided tape to fix the back of the buds on the operation board, and conduct high-energy carbon ion beam irradiation experiments. The radiation dose is 40Gy.

[0040] The branches treated with high-energy carbon ion beams were wrapped with wet newspaper, and stored in a refrigerator at 4°C until use.

[0041]Select a strong biennial wild kiwi rootstock, cut it with branch shears at a distance of 90 cm from the ground, and cut obliquely 1.5 to 2 cm toward the cambium at about 5 mm on the east and west sides of the main section. The lower end of the scion irradiated by the high-energy carbon ion beam is cut into a horseshoe-shaped slope to expose the cambium, the cut surface is 1.5-2.5 c...

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Abstract

The invention provides a grafting method for irradiating actinidia chinensis branches by means of a high-energy carbon ion beam, and relates to the technical field of plant mutagenesis. The method comprises the following steps that 1, the high-energy carbon ion beam is used for irradiating the actinidia chinensis branches to obtain irradiated scions, wherein the radiation dosage is 10-40 Gy; 2, the irradiated scions obtained in the step 1 are grafted onto actinidia chinensis stocks, and after binding is conducted, irradiated actinidia chinensis grafted seedlings are obtained. According to thegrafting method for irradiating the actinidia chinensis branches by means of the high-energy carbon ion beam, firstly, the actinidia chinensis branches are subjected to high-energy carbon ion beam irradiation, and then multiple buds are grafted, so that the grafting survival rate of the irradiated actinidia chinensis branches is maintained to be about 40%-80%. By means of the method, the problem of a low survival rate of actinidia chinensis branches subjected to irradiation mutagenesis treatment is solved. Treating the actinidia chinensis buds by means of high-energy carbon ion beam irradiation in combination with grafting provides a material basis for biological studies of actinidia chinensis high-energy carbon ion beam irradiation mutagenesis and fast breeding of new actinidia chinensisspecies.

Description

technical field [0001] The invention belongs to the technical field of plant mutagenesis, and in particular relates to a grafting method for kiwi fruit branches irradiated by high-energy carbon ion beams. Background technique [0002] As a new kind of fruit with the same origin of medicine and food, kiwi fruit is famous for its rich nutrition and health care. In addition to organic substances such as actinine, proteolytic enzymes, tannin pectin and sugars, as well as trace elements such as calcium, potassium, selenium and zinc, and 17 kinds of amino acids needed by the human body, kiwifruit also contains rich vitamin C, grape acid, Fructose, citric acid, malic acid, fat, especially the content of vitamin C ranks first among all kinds of fruits. Long-term consumption of kiwi fruit has a good preventive effect on frequently-occurring diseases. Kiwi fruit is known as the "king of fruits", "king of Vc", and "healthy miracle fruit", and is loved by people. [0003] At present, ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01G2/30
CPCA01G2/30A01H1/06
Inventor 杨爱红周利斌刘腾云胡淼刘淑娟李彦强骆善伟余发新
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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