Application of OsNMCP1 gene in controlling drought tolerance in rice

A rice and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of affecting plant growth, restricting plant growth and development, increasing crop production and income, etc.
CN109112142AInactive Publication Date: 2019-01-01HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2019-01-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of plant gene engineering, particularly relates to application of OsNMCP1 gene in controlling drought tolerance in rice. According to the invention, a candidate gene screening method is adopted to clone a rice drought-tolerant response gene OsNMCP1 by reverse genetics. The nucleotide sequence of the gene is shown in SEQ NO:1, and the protein sequence encoded by the gene is shown in SEQ NO:3. The phenotypic identification of drought stress at seedling stage and heading stage indicates that overexpression of OsNMCP1 gene causes an improvement of the drought resistance of transgenic rice, while the deletion mutation of OsNMCP1 gene results in a significant decrease of rice drought resistance. In the invention, the biological function of the gene and the pathways and methods of application with the same are demonstrated.
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Description

technical field

[0001] The invention belongs to the technical field of plant genetic engineering. It specifically relates to the application of OsNMCP1 gene in controlling drought tolerance of rice. The present invention adopts the method of candidate gene screening, through the means of reverse genetics, to clone the rice drought-tolerant response gene OsNMCP1, and the results of drought stress phenotype identification at the seedling stage and adult plant stage show that overexpressing the OsNMCP1 gene can improve the performance of rice in drought environment. The development and growth of the lower root, while the deletion of the OsNMCP1 gene will lead to the growth retardation of the rice root, the development of the cells is hindered, and the root length is shortened, which confirms the function and application of the gene. Background technique

[0002] Plants do not have the ability to move like animals, so when abiotic adversity (such as drought, waterlogging, high ...

Claims

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