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Application of rice osmpkk10-2 gene in improving rice disease resistance and drought resistance

A gene, rice technology, applied in the field of plant genetic engineering, can solve the problems of food security situation impact, yield and quality decline, etc.

Inactive Publication Date: 2020-09-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rice is facing many challenges in actual production. The impact of diseases and abiotic stress often leads to a decline in yield and quality, which has a very serious impact on food security.

Method used

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  • Application of rice osmpkk10-2 gene in improving rice disease resistance and drought resistance
  • Application of rice osmpkk10-2 gene in improving rice disease resistance and drought resistance
  • Application of rice osmpkk10-2 gene in improving rice disease resistance and drought resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Analysis of the expression pattern of the OsMPKK10-2 gene after biotic and abiotic stress treatments

[0024] It has been reported that some mitogen-activated protein kinase genes in plants are involved in the regulation of plant disease resistance and / or drought resistance (Ning et al., 2010; Meng and Zhang, 2013). Benzothiadiazol (BTH) is a functional analog of the plant hormone salicylic acid (SA), which can activate the plant's disease resistance signaling pathway when exogenously applied to enhance plant disease resistance ( et al., 1996). ABA plays an important role in plant resistance to drought, high salinity and other stress responses (Zhu, 2002).

[0025] In order to verify whether the OsMPKK10-2 gene is involved in the regulation of rice resistance to disease and drought, the researchers of the present invention sprayed benzothiadiazole (BTH) or abscisic acid (ABA) on four-leaf stage japonica rice variety Zhonghua 11, and inoculated thin strips. ...

Embodiment 2

[0026] The test results showed that the expression of OsMPKK10-2 gene was rapidly induced after treatment with BTH and inoculation with S. finestripes; and the expression of OsMPKK10-2 gene was also rapidly induced after ABA treatment and drought stress ( figure 2 ). This result suggested that the OsMPKK10-2 gene may be involved in the regulation of rice resistance to thin stripe disease and drought stress, and enhancing the expression of this gene may improve the disease resistance and drought resistance of rice. Example 2: Determining the sequence and structure of the OsMPKK10-2 gene in rice variety Zhonghua 11

[0027] 1. Cloning of the sequence of the OsMPKK10-2 gene in the rice variety Zhonghua 11

[0028] According to the literature (Hamel et al., 2006), the LOC_Os03g12390 site in the rice genome sequence database (http: / / rice.plantbiology.msu.edu / ) contains the sequence information of the OsMPKK10-2 gene in the rice variety Nipponbare, Its corresponding cDNA sequence...

Embodiment 3

[0036] Example 3: Functional verification of OsMPKK10-2 gene

[0037] The invention adopts the genetic transformation method mediated by Agrobacterium to further verify the function of the gene by overexpressing the OsMPKK10-2 gene in the rice variety Zhonghua 11.

[0038] 1. Construction of genetic transformation vector

[0039] The starting vector used in the present invention is the commonly used vector pU1301 ( Figure 5 , Qiu et al., 2007). It is an Agrobacterium-mediated genetic transformation vector carrying a maize ubiquitin promoter with constitutive and overexpression characteristics.

[0040] Using the T-A cloning vector containing the full-length cDNA sequence of the OsMPKK10-2 gene in Example 2-2 as a template, use the PCR primer OsMPKK10-2-oe-F2 (5'-GG GGTACC TCCAACCGATGGCTCTC-3′) (the underline is the KpnI restriction site) and OsMPKK10-2-oe-R2 (5′-CG GGATCC TAGATCGCCGCTCTGCT-3') (the underline is the BamHI restriction site) to amplify the entire coding re...

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Abstract

The invention belongs to the technical field of plant genetic engineering, specifically relates to applications of a rice OsMPKK10-2 gene in improvement of the disease resistance and the drought resistance of rice, and provides isolation cloning and function verification of the DNA fragment of the rice disease-resistance related gene OsMPKK10-2, wherein the nucleotide sequence of the OsMPKK10-2 gene is represented by SEQ ID NO:1, the sequence of the protein encoded by the gene is represented by SEQ ID NO:2, and the encoded product of the protein is a mitogen-activated protein kinase. Accordingto the present invention, the resistance of the transgenic plant over-expressing the OsMPKK10-2 to bacterial leaf streak and drought stress is significantly enhanced.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. It specifically relates to the application of rice OsMPKK10-2 gene in improving rice disease resistance and drought resistance. The invention includes the isolation and cloning of the rice OsMPKK10-2 gene, and the functional verification and application of regulating rice response to pathogen invasion and drought stress. OsMPKK10-2 gene promotes disease and drought resistance in rice. The ability of transgenic plants overexpressing OsMPKK10-2 gene to resist rice bacterial stripe disease and drought stress was significantly enhanced. Background technique [0002] Plants are affected by many factors in the growth environment during their growth, including biotic and abiotic factors. Biological factors mainly refer to various pathogens, such as bacteria, fungi, viruses and nematodes. Abiotic factors include various adverse factors such as drought, low temperature, high salinity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/12
CPCC12N9/12C12N15/8273C12N15/8281C12Y207/12002
Inventor 王石平马海港
Owner HUAZHONG AGRI UNIV