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PEFC-based self-powered miRNA biosensor and application thereof

A biosensor, self-powered technology, applied in the field of biosensing, can solve the problems of low sensitivity of microarray detection method, large demand for samples, need to be separated and purified, etc., and achieve the effects of improving detection sensitivity, simple detection equipment and low cost

Inactive Publication Date: 2019-01-01
QINGDAO AGRI UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

Northern blotting has low sensitivity, time-consuming and labor-intensive, and requires a large amount of samples
Microarray detection also has the disadvantage of low sensitivity and low specificity
RT-PCR has high specificity and high sensitivity, but the method is cumbersome to operate and RNA needs to be separated and purified
The small size of miRNA also limits the direct application of traditional RT-PCR, and the high sequence homology among miRNA family members also makes quantitative analysis a challenge

Method used

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  • PEFC-based self-powered miRNA biosensor and application thereof
  • PEFC-based self-powered miRNA biosensor and application thereof
  • PEFC-based self-powered miRNA biosensor and application thereof

Examples

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Embodiment 1

[0040] Self-powered miRNA biosensor based on PEFC for the detection of miRNA-141

[0041] (1)C 3 N 4 Preparation of:

[0042] Mix melamine and urea at a mass ratio of 1:1, place in a tube furnace, raise the temperature to 550°C at 3°C / min and maintain it for 2 hours, the obtained yellow blocky solid is C 3 N 4 , ground to powder for later use;

[0043] (2)g-C 3 N 4 Preparation of:

[0044] Get the C prepared in the step (1) of 1g 3 N 4 , added to 100mL, 5M HNO 3 Reflux at 110°C for 8 hours, centrifuge and wash the white product obtained at 9000rpm several times until neutral, and centrifuge the obtained washing solution at 3000rpm for 15min, take the supernatant to obtain a white bluish colloid, which is the stripped g-C 3 N 4 Nanosheets;

[0045] (3) AuNPs-g-C 3 N 4 Preparation of:

[0046] Take 2 mL of the stripped g-C prepared in step (2) 3 N 4 The nanosheets were dissolved in 4 mL of secondary water and sonicated for 0.5 h, and 20 μL of HAuCl 4 Added to t...

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Abstract

The invention relates to a PEFC-based self-powered miRNA biosensor and application thereof, belonging to the technical field of biological sensing. One end of a hairpin DNA partially complementary with miRNA is modified with Cds QDs, and the Cds QDs can sensitize a photoelectrochemical material that is g-C3N4. After the target miRNA and a complementary chain thereof form a rigid double helix structure through hybridization and pairing, the Cds QDs is brought far from the surface of the g-C3N4 so that the g-C3N4 sensitizing effect of the Cds QDs is weakened, electrons flowing from an anode to acathode are reduced, and the open-circuit voltage of the PEFC changes, thus achieving miRNA detection. The sensor does not need an extra power supply device during detection and is simple and convenient to assemble, low in cost, high in interference resistance, and high in selectivity because of complementary pairing effects of DNA chains. The sensor can achieve simple, rapid, sensitive and efficient miRNA detection.

Description

technical field [0001] The invention relates to a PEFC-based self-powered miRNA biosensor and an application thereof, belonging to the technical field of biosensing. Background technique [0002] Photoelectric biofuel cell (PEFC) is a special fuel cell, which can provide sustainable energy by light excitation under mild conditions, and has attracted extensive attention. The self-powered biosensor based on PEFC is a kind of sensor that uses the output of battery performance as the analysis and detection signal, and the sensor signal is proportional to the concentration of the detected and analyzed pollutants. Compared with traditional sensors, photoelectric self-powered biosensors do not need to apply additional power during the detection process, and its specific advantages are mainly manifested in: (1) The equipment is simple. The detection process is different from the traditional electrochemical detection three-electrode system, only two electrodes are needed, that is, t...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 李峰盖盼盼张淑霞刘晓娟侯婷
Owner QINGDAO AGRI UNIV
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