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Memory improving pentapeptide and application thereof

A technology for improving memory and drugs, which is applied in the field of pentapeptides. It can solve the problems that the peptide sequence is not deep enough and cannot be supplemented daily, and achieve good inhibitory activity, obvious memory improvement effect, and good synergistic memory improvement effect.

Inactive Publication Date: 2019-01-04
广东华肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the specific polypeptide sequence of cerebrolysin is not in-depth, and the drug enters the body by injection, so it cannot be supplemented daily

Method used

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  • Memory improving pentapeptide and application thereof
  • Memory improving pentapeptide and application thereof
  • Memory improving pentapeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the inhibitory activity of pentapeptide PAYCS to acetylcholinesterase

[0031] The assay method of acetylcholinesterase inhibitory activity is as follows:

[0032] Add 30 μL of acetylcholine (7.5 mM), 125 μL of DTNB (3 mM), 40 μL of HEPES (pH 8.0, 50 mM, containing 0.1% bovine serum albumin) and 50 μL of samples into a 96-well plate, mix well and incubate at 37°C for 15 min. After the incubation, 30 μL of AChE (0.055 U / mL) was added to start the assay. The assay process was carried out in a microplate reader. The measurement wavelength is 412nm, and the measurement time is 15min. The formula for calculating the inhibitory activity of the sample on acetylcholinesterase is:

[0033] Acetylcholinesterase inhibitory activity (%)=[1-(A 样品 -A 样品空白 ) / (A 对照 -A 对照空白 )]×100%

[0034] Among them, sample A is the absorbance value of the sample group, the blank of sample A is the sample group without adding enzyme, the control A is the group without adding enzy...

Embodiment 2

[0037] Example 2: Effect of pentapeptide PAYCS on memory improvement in scopolamine-induced dementia mouse model

[0038] Select 48 SPF grade Kunming mice, half male and half male, weighing 18-22 g, provided by the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine. The feeding environment of the experimental mice was room temperature (21±2)°C, the relative humidity was controlled at 50-60%, and the light and dark were alternated for 12 / 12 hours. During the experiment, the mice were allowed to eat and drink freely. The mice were placed in the laboratory to adapt to the environment 7 days before the experiment. 48 mice were randomly divided into 4 groups (12 in each group, n=12), respectively blank control group, scopolamine group (model group), piracetam group (positive control group), PAYCS (0.2mmol / kg ). The blank group and the model group were given equal volumes of distilled water, the positive control group was given piracetam (400mg / kg),...

Embodiment 3

[0053] A tablet for improving memory, containing 15wt% of the pentapeptide of the present invention and 85wt% of auxiliary materials, wherein the auxiliary materials are any one or more of starch, sucrose, and maltodextrin, preferably starch and magnesium stearate.

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PUM

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Abstract

The invention discloses memory improving pentapeptide and an application thereof. The amino acid sequence of the pentapeptide is Pro-Ala-Try-CysSer. The memory improving pentapeptide is prepared witha solid-phase chemical synthesis method, has better inhibitory activity for acetylcholinesterase, has an obvious memory improving effect on a dementia mouse model, can be independently used for preparing memory improving drugs or memory improving health care products, and can also be compounded with memory improving related substances in the prior art for use so as to realize better synergistic memory improving effect.

Description

technical field [0001] The invention belongs to the field of health care products, and specifically relates to a pentapeptide capable of inhibiting the activity of acetylcholinesterase in vitro and improving the memory ability of dementia mice in vivo and its application. Background technique [0002] Acetylcholinesterase (AChE) is a key enzyme to maintain the function of cholinergic system. It hydrolyzes acetylcholine into choline and acetic acid. If acetylcholine as a neurotransmitter is excessively degraded by acetylcholinesterase, it will not be able to bind to the receptors on the post-synaptic membrane normally, causing disorder in the transmission of nerve signals between synapses, and eventually leading to memory loss. It can be seen that moderate acetylcholinesterase can ensure the normal circulation of choline and acetyl-CoA, so that acetylcholine can maintain an optimal level in the nervous system, thus showing better memory. [0003] However, with age, some neu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A23L33/18A61K38/08A61P25/28
CPCC07K7/06A23L33/18A23V2002/00A61K38/00A61P25/28A23V2200/322A23V2250/55
Inventor 赵谋明赵甜甜苏国万赵翊君
Owner 广东华肽生物科技有限公司
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