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Method of efficiently synthesizing solvents and organic acids by butanol fermentation

A butanol fermentation and organic acid technology, applied in the field of microbial fermentation, can solve the problems of low butyric acid/butanol, low butyric acid concentration, inability to obtain high value-added products of in-situ synthesis of butyl butyrate, and achieve efficient synthesis , increase production and reduce the cost of fermentation raw materials

Active Publication Date: 2019-01-04
HUAIYIN INSTITUTE OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the concentration of butyric acid is low at the end of fermentation, and the butyric acid / butanol ratio is low, so it is impossible to obtain in-situ synthesis of butyl butyrate or other high value-added products

Method used

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  • Method of efficiently synthesizing solvents and organic acids by butanol fermentation
  • Method of efficiently synthesizing solvents and organic acids by butanol fermentation
  • Method of efficiently synthesizing solvents and organic acids by butanol fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 2

[0039] Embodiment 2: Butanol fermentation process with 0.2 g / L vanillin added

[0040] The preparation process of the activation solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0041] The preparation process of the Clostridium acetobutylicum seed solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0042] The Clostridium acetobutylicum seed solution was transferred to a 5 L anaerobic fermentation tank with an inoculum of 10% (v / v), and the anaerobic fermentation tank was filled with 3 L Clostridium fermentation medium (where the glucose concentration was 60 g / L, the vanillin concentration is 0.2 g / L), the fermentation temperature is 37°C, and the pH control strategy during the fermentation process is expressed as follows: During the acid production period (~0-15 h), the pH is automatically controlled to 5.0 by the control cabinet , using 14% (w / v) ammonia water; during the solventogenic phase (~15-48 h)...

Embodiment approach 3

[0046] Embodiment 3: Butanol fermentation process with 0.2 g / L vanillic acid added

[0047] The preparation process of the activation solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0048] The preparation process of the Clostridium acetobutylicum seed solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0049] The Clostridium acetobutylicum seed solution was transferred to a 5 L anaerobic fermentation tank with an inoculum of 10% (v / v), and the anaerobic fermentation tank was filled with 3 L Clostridium fermentation medium (where the glucose concentration was 60 g / L, the concentration of vanillic acid is 0.2 g / L), the fermentation temperature is 37°C, and the pH control strategy during the fermentation process is expressed as follows: During the acid production period (~0-15 h), the pH is automatically controlled to 5.0 by the control cabinet, 14% (w / v) ammonia was utilized; during the solventogenic pha...

Embodiment approach 4

[0053] Embodiment 4: Butanol fermentation process with addition of 0.1 g / L vanillin and 0.1 g / L vanillic acid

[0054] The preparation process of the activation solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0055] The preparation process of the Clostridium acetobutylicum seed solution is exactly the same as that in Embodiment 1, and will not be repeated here.

[0056]The Clostridium acetobutylicum seed solution was transferred to a 5 L anaerobic fermentation tank with an inoculum of 10% (v / v), and the anaerobic fermentation tank was filled with 3 L Clostridium fermentation medium (where the glucose concentration was 60 g / L, the concentration of vanillin is 0.1 g / L, the concentration of vanillic acid is 0.1 g / L), the fermentation temperature is 37°C, and the pH control strategy during the fermentation process is expressed as follows: During the acid production period (~0-15 h) , the pH is automatically controlled to 5.0 through the con...

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Abstract

The invention relates to the field of microbial fermentation and discloses a method of efficiently synthesizing solvents and organic acids by butanol fermentation. The method comprises the steps of S1, activating in 5% corn mash medium, Clostridium acetobutylicum collected in a spore tube, transferring the activated article to a Clostridium growth medium, and culturing to obtain high-activity Clostridium acetobutylicum seed liquid; S2, transferring the high-activity Clostridium acetobutylicum seed liquid to a Clostridium fermenting medium, and producing butanol, acetone, ethanol, acetic acid and butyric acid, wherein the first phase of fermentation is an acid producing period, pH of a fermenting liquid in the acid producing period is controlled to 5.0, and pH is not controlled after fermentation is performed in a solvent producing period; S3, adding phenols into the Clostridium fermenting medium, and investigating kinetic characteristics of butanol in the fermenting process in a 5 L anaerobic fermentation tank. By simply adding a little quantity of fermentation inhibitors, phenols, both solvent production and organic acid production are increased, and butanol fermenting performanceis finally improved.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for efficiently synthesizing solvents and organic acids through butanol fermentation. Background technique [0002] Acetone-butanol-ethanol fermentation, also known as ABE (Acetone-Butanol-Ethanol) fermentation or butanol fermentation, is the use of Clostridium difficile ( Clostridia ) fermentation process for solvent production of acetone, butanol and ethanol under strictly anaerobic conditions. Butanol, the main product of fermentation, is an important platform compound, and at the same time, it is also a new type of clean and renewable liquid fuel and diesel combustion improver with great potential. For a long time, people prefer to use ABE fermentation to obtain high-purity butanol solvent. However, the lipophilicity of butanol will destroy the phospholipid structure of the cell membrane, enhance the fluidity of the cell membrane, and greatly damage the cells....

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P7/16C12P7/28C12P7/52C12P7/54C12R1/145
CPCC12P7/065C12P7/16C12P7/28C12P7/52C12P7/54Y02E50/10
Inventor 罗洪镇郑盼丽谢方杨荣玲刘帅曾庆伟
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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