A kind of processing technology of leather solid waste
A technology of solid waste and treatment process, which is applied in the direction of solid waste removal, etc., and can solve problems such as not being able to grow well
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Embodiment 1
[0049] 1) Decalcification: soak 4.2kg of cured leather waste in 10L of 1wv% ammonium chloride solution for 5 hours, remove, rinse with 40L of water, and dry;
[0050] 2) Pulverization: pulverize the solid waste after decalcification in step 1) to a particle size of ≤0.25 cm to obtain powdery waste;
[0051] 3) Biological enzymatic hydrolysis: fill carbonic acid into deionized water to make pH = 3.5 to obtain a carbonic acid solvent, add 7 kg of the carbonic acid solvent to 1 kg of powdery waste, add 0.7 g of cysteine and stir, add 8.82 kU hydrolyze with cellulase at 30°C for 2h; add carbonic acid to each carbonic acid solution until the pH is 3.5, add 3.5kU of bromelain to hydrolyze at 30°C for 0.5h; add sodium carbonate to adjust the pH to 8.3, then add 8.4kU of lipase Enzymolysis at 30°C for 2 hours to obtain the enzymatic hydrolysis solution;
[0052] 4) Microalgae preplanting: culture Euglena gracilis in 50mL Hunter trace element culture medium, insert 0.5×10 9 Cells o...
Embodiment 2
[0055] 1) Decalcification: immerse 4.45kg of cured leather waste in 10L of 1.5wv% ammonium chloride solution for 5 hours, remove, rinse with 40L of water, and dry;
[0056] 2) Pulverization: pulverize the solid waste after decalcification in step 1) to a particle size of ≤0.25 cm to obtain powdery waste;
[0057] 3) Biological enzymolysis: fill carbonic acid into deionized water to make pH = 4.5 to obtain a carbonic acid solvent, add 8 kg of the carbonic acid solvent to 1 kg of powdery waste, stir after adding 0.9 g of cysteine, add 24 kU of Cellulase, enzymatic hydrolysis at 30°C for 2h; add carbonic acid to each carbonic acid solution to pH 4.5, add 80kU bromelain at 30°C for 0.5h; add sodium carbonate to adjust the pH to 8.3, add 48kU lipase at 30°C Decompose for 2 hours to obtain the enzymatic solution;
[0058] 4) Microalgae preplanting: culture Euglena gracilis in 100mL Hunter trace element culture medium, insert 1×10 9 Cells of Euglena cellulosus, after culturing for ...
Embodiment 3
[0061] 1) Decalcification: soak 4.0kg of cured leather waste in 10L of 2wv% ammonium chloride solution for 5 hours, remove, rinse with 40L of water, and dry;
[0062] 2) Pulverization: pulverize the solid waste after decalcification in step 1) to a particle size of ≤0.25 cm to obtain powdery waste;
[0063] 3) Biological enzymolysis: fill carbonic acid into deionized water to make pH=5.5 to obtain a carbonic acid solvent, add 10 kg of the carbonic acid solvent to 1 kg of powdery waste, stir after adding 1.2 g of cysteine, add 70 kU of Cellulase, enzymatic hydrolysis at 30°C for 2h; add carbonic acid to each carbonic acid solution to pH 5.5, add 120kU bromelain at 30°C for 0.5h; add sodium carbonate to adjust the pH to 8.0, add 78kU lipase at 30°C Decompose for 2 hours to obtain the enzymatic solution;
[0064] 4) Microalgae preplanting: culture Euglena gracilis in 150mL Hunter trace element culture medium, insert 1×10 9 Cells of Euglena cellulosus, after cultivating for 3 da...
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