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A method for producing butyric acid from corn cob full components

A technology for the production of butyric acid and corn cob, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of expensive and wasteful ionic liquids, and achieve the effect of realizing the utilization of all components

Active Publication Date: 2021-07-20
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is relatively wasteful to pretreat biomass with only a single component, as ionic liquids are still too expensive for many commercial applications

Method used

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  • A method for producing butyric acid from corn cob full components

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Such as figure 1 Shown, method of the present invention comprises the steps:

[0028] (1) Wash and dry the corncobs and crush them, with a particle size of 80-120 mesh;

[0029] (2) Preprocessing:

[0030] Take 0.3 g of corncob powder and add it to 5 mL [Mmim]DMP and 16 wt% γ-valerolactone mixture (16 wt% refers to the mass of γ-valerolactone / ([Mmim]DMP+γ-valerolactone total mass)), heated at 90°C in a 50 mL three-neck round bottom flask under nitrogen atmosphere, and reacted for 4 h under magnetic stirring at 1000 rpm. After the reaction was finished, the reaction mixture was diluted with the same volume of deionized water, which was used as an anti-solvent to precipitate the pretreated corncob powder. The resulting corncob residue and filtrate were separated by suction filtration.

[0031] (3) The corncob residue was repeatedly washed with deionized water to remove possible [Mmim]DMP and γ-valerolactone residues, and dried at 65°C for 10 hours to obtain the pretre...

Embodiment 2

[0039] (1) Wash and dry the corncobs and crush them, with a particle size of 80-120 mesh;

[0040] (2) Preprocessing:

[0041] Take 0.3 g of corncob powder, add it to 10 mL [Mmim]DMP and 14 wt % γ-valerolactone mixture, heat in a 50 mL three-necked round bottom flask at 90 °C under nitrogen atmosphere, and react under 1000 rpm magnetic stirring 3 h. After the reaction was finished, the reaction mixture was diluted with the same volume of deionized water, which was used as an anti-solvent to precipitate the pretreated corncob powder. The resulting corncob residue and filtrate were separated by suction filtration.

[0042] (3) The corncob residue was repeatedly washed with deionized water to remove possible [Mmim]DMP and γ-valerolactone residues, and dried at 65°C for 10 hours to obtain the pretreated corncob powder.

[0043] Put 0.25 g of pretreated corncob powder into a 25 mL Erlenmeyer flask, add 5 mL of 50 mM acetate buffer (pH4.8), 45 FPU / g cellulase (purchased from Ning...

Embodiment 3

[0050] (1) Wash and dry the corncobs and crush them, with a particle size of 80-120 mesh;

[0051] (2) Preprocessing:

[0052] Take 0.3 g corncob powder, add it to 15 mL [Mmim]DMP and 10 wt % γ-valerolactone mixture, heat in a 50 mL three-neck round bottom flask at 110 °C under nitrogen atmosphere, and react under 1000 rpm magnetic stirring 3 h. After the reaction was finished, the reaction mixture was diluted with the same volume of deionized water, which was used as an anti-solvent to precipitate the pretreated corncob powder. The resulting corncob residue and filtrate were separated by suction filtration.

[0053] (3) The corncob residue was repeatedly washed with deionized water to remove possible [Mmim]DMP and γ-valerolactone residues, and dried at 65°C for 10 hours to obtain the pretreated corncob powder.

[0054] Put 0.25 g of pretreated corncob powder into a 25 mL Erlenmeyer flask, add 5 mL of 50 mM acetate buffer (pH4.8), 45 FPU / g cellulase (purchased from Ningxia ...

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Abstract

The invention discloses a method for producing butyric acid by utilizing the whole components of corn cobs. Using γ-valerolactone and 1,3-dimethylimidazole dimethyl phosphate ([Mmim]DMP) as solvents, corn cobs were pretreated, and water was used as anti-solvent to precipitate corn cobs, and the residue was obtained by suction filtration , using dried corn cob residue as substrate, cellulase for enzymatic hydrolysis to obtain fermentable sugar; lignin extracted from the pretreatment solvent was precipitated with acid and used to prepare macropores for clostridium tyrobutyricum Calcium alginate-lignin microspheres, so that it can use fermentable sugar to ferment to produce butyric acid, and realize the comprehensive utilization of all components of corn cob. The present invention effectively utilizes the full-component fermentation of corncobs to produce butyric acid, and the combined use of [Mmim]DMP and γ-valerolactone greatly improves the pretreatment effect, enhances the enzymatic hydrolysis efficiency of corncobs, and obtains high yield The fermentable sugar of Clostridium tyrobutyricum was embedded in macroporous calcium alginate-lignin microspheres, and the production of butyric acid was also improved compared with free cells.

Description

technical field [0001] The invention relates to the technical field of comprehensive utilization of corncobs as biomass resources, in particular to a method for producing butyric acid by utilizing all components of corncobs. Background technique [0002] Corn is one of the international food crops and a tradable commodity. In China, maize is widely grown, with an output of about 220 million tons in 2016. Based on the ratio between kernels and cobs, the sweet corn processing industry is expected to produce approximately 40 million tons of cob by-products each year. In South Africa, maize is the main export commodity and is mainly used to produce second-generation maize products such as cornflakes, cornmeal and glucose. Due to the large amount of corn consumed every year, corn cobs have become one of the largest agricultural wastes in South Africa, with an annual output of about 9 million tons, accounting for nearly 20% of corn residues. In the past, most corncobs were burn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/52C12P19/14C12P19/02C12N11/10C12N11/04C12R1/145
CPCC12N11/04C12N11/10C12P7/52C12P19/02C12P19/14C12P2201/00C12P2203/00
Inventor 江凌郑文秀赵倩如黄和朱丽英
Owner NANJING TECH UNIV
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