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Method and kit for detecting atrazine, chlorpyrifos and triazolone based on SERS technology

A detection kit, atrazine technology, applied in measurement devices, Raman scattering, instruments, etc., can solve problems such as low detection sensitivity, achieve high stability, and overcome the effects of long preparation cycles

Active Publication Date: 2019-01-11
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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AI Technical Summary

Problems solved by technology

In view of the current development status of SERS immunolabeling technology, in order to realize the simultaneous detection of a variety of pesticide small molecules, on the one hand, it is necessary to further develop the multi-component labeling technology of SERS, screen suitable marker molecules with different Raman characteristic signals, and prepare highly sensitive and multi-component pesticides. Component-labeled probes; on the other hand, study the detection mode of SERS immunolabeling technology to overcome the technical defect of low detection sensitivity caused by the immuno-sandwich method with few active sites for binding small molecules

Method used

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  • Method and kit for detecting atrazine, chlorpyrifos and triazolone based on SERS technology

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preparation example Construction

[0038] In some embodiments, the preparation method of the artificial antigen of atrazine, chlorpyrifos and triadimefon includes:

[0039] The hapten of the pesticide molecule is activated with NHS and EDC in sequence and then coupled with the carrier protein;

[0040] The pesticide molecule hapten is selected from any one of atrazine, chlorpyrifos, and triadimefon.

[0041] In some embodiments, the molar ratio of the hapten of the pesticide molecule to NHS and EDC is 1: (1.7-2.3): (2.7-3.3);

[0042] In some embodiments, the molar ratio of the pesticide molecule hapten to NHS and EDC is 1:2:3.

[0043] In some embodiments, the carrier protein is BSA.

[0044] According to another aspect of the present invention, the present invention also relates to a method for detecting pesticides using the kit as described above, the method is used for detecting one or more of atrazine, chlorpyrifos, and triadimefon, the method include:

[0045] The substance to be detected is competitively combined w...

Embodiment 1

[0048] 1. Synthesis of nano gold sol

[0049] Firstly, 300 mL of 0.01% chloroauric acid solution is heated to boiling, and a certain amount of 1% trisodium citrate aqueous solution is quickly added, and by changing the addition amount of sodium citrate, nano gold sols with different particle diameters are obtained. It starts to be a little blue, then light blue, blue, red appears after heating, and a transparent orange-red appears after boiling for 7-10 minutes, wherein the molar ratio of the trisodium citrate to the chloroauric acid is 4.3.

[0050] 2. Preparation of signal labeled probe based on AuNPs / carrier protein / target

[0051] Triazophos hapten carrier protein, atrazine hapten carrier protein, and chlorpyrifos hapten carrier protein were selected respectively, and reconstitution techniques such as covalent bonding and electrostatic self-assembly were used to prepare gold nanospheres (AuNPs) / carrier protein / Three different signal-labeled probes composed of targets.

[0052] (...

Embodiment 2

[0062] 1. Synthesis of nano gold sol

[0063] Firstly, 300 mL of 0.01% chloroauric acid solution is heated to boiling, and a certain amount of 1% trisodium citrate aqueous solution is quickly added, and by changing the addition amount of sodium citrate, nano gold sols with different particle diameters are obtained. It starts to be a little blue, then light blue, blue, red after heating, and a transparent orange-red color appears after boiling for 7-10 minutes. In the experiment, it is found that the molar ratio of trisodium citrate: chloroauric acid is 4.9. Stable storage for more than half a year.

[0064] 2. Preparation of signal labeled probes based on AuNPs / carrier protein / target

[0065] Triazophos hapten carrier protein, atrazine hapten carrier protein, and chlorpyrifos hapten carrier protein were selected respectively, and reconstitution techniques such as covalent bonding and electrostatic self-assembly were used to prepare gold nanospheres (AuNPs) / carrier protein / Three di...

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Abstract

The invention relates to the field of pesticide detection, in particular to a method and a kit for detecting atrazine, chlorpyrifos and triazolone based on an SERS technology. The kit is used for detecting one or more of the atrazine, the chlorpyrifos and the triazolone, and comprises a solid-phase carrier and labeled probes; the solid-phase carrier is coated with molecularly imprinted polymer membranes of the atrazine, the chlorpyrifos and the triazolone; the labeled probes are metal nanoparticles coupled with artificial antigens and labeled molecules; and the artificial antigen is one of artificial antigens of the atrazine, the chlorpyrifos and the triazolone, and the labeled molecules on the labeled probes can enable the atrazine, the chlorpyrifos and the triazolone to be distinguishedwhen the atrazine, the chlorpyrifos and the triazolone are detected by using the SERS technology. By combining a molecular imprinting technology with a Raman signal amplification probe technology, thedetection method for simultaneously detecting the three kinds of pesticides is established.

Description

Technical field [0001] The invention relates to the field of pesticide detection, in particular to a method and a kit for detecting atrazine, chlorpyrifos and triadimefon based on SERS technology. Background technique [0002] Surface-enhanced Raman spectroscopy (SERS) technology, as an emerging spectroscopic analysis technology, is widely used in the detection of pharmaceutical analysis, biological analysis, environmental pollutants and other fields due to its simple operation and fast detection speed. In recent years, SERS has shown great application value in the field of rapid and real-time detection of pesticide residues. There have been many reports on the detection of pesticides by SERS, but these detection methods largely depend on the relationship between the adsorbed molecules and the SERS substrate. The interaction and the surface plasmon resonance effect of the SERS active substrate. Therefore, the sensitivity, stability and uniformity of the SERS substrate are the ke...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 佘永新颜朦朦王静王淼王珊珊郑鹭飞曹振邵勇
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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