A gold standard nucleic acid test strip for rapid detection of aquatic diseases and its preparation method

Abstract

The invention relates to a gold-labelled nucleic acid test strip for quickly detecting aquatic diseases. The gold-labelled nucleic acid test strip comprises a sample pad, a gold-labelled pad containing probe Probe1-AuNPs composite, a nitrocellulose membrane and a water absorbent pad; a detection line and a quality control line which are separated from each other are respectively adsorbed on the nitrocellulose membrane; the detection line is composed of a probe2, and the quality control line is composed of the probe 1'; the probe1 and the probe2 are respectively designed by selecting a characteristic conserved segment from a vibrio splendidus toxicity gene sequence and using the 5' end and 3' end of the characteristic conserved segment, and then a complementary sequence Probe1' is designedaccording to the probe1. A nano-gold nucleic acid probe is used as the label in the development of the gold-labelled nucleic acid test strip, and the vibrio splendidus is detected through a thin-filmchromatography measure, thereby providing simple, convenient and efficient detection method for the disease control of the vibrio splendidus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a gold standard nucleic acid test strip for rapid detection of aquatic diseases and a preparation method thereof. Background technique [0002] Vibrio splendidus ( Vibrio splendidus ) belongs to the Vibrio family and belongs to the genus Vibrio. It is one of the main pathogenic bacteria of mariculture animals. It can infect hosts such as fish, echinoderms, bivalve molluscs and crustaceans, leading to the occurrence of aquaculture diseases. The rotting skin syndrome of sea cucumbers caused by it can lead to pathological reactions such as mouth swelling, anorexia, body surface ulceration, body atrophy, and viscera discharge, which eventually lead to the death of sea cucumbers and bring huge economic losses to aquaculture. Early detection and diagnosis of the pathogen is an effective means to prevent the occurrence of such diseases. Therefore, it is very important to research and devel...

AI Technical Summary

Problems solved by technology

However, there are many shortcomings and deficiencies in these methods: traditional pathogenic bacteria culture and physiological and biochemical detection need to be isolated and cultivated, morphological observation, physiological and biochemical index identification, etc., which are time-consuming and labor-intensive, with limited accuracy and reliability; detection based on molecular biology technology Although the method is fast and sensitive, it is prone to false positives, and requires professional technicians and special equipment, which limits its application in the aquaculture industry; the detection method based on immunology is a relatively simple detection method, but all The preparation of monoclonal antibodies is inseparable, and the preparation process of monoclonal antibodies is complicated and the production cycle is long, which has become the bottleneck of the development of this detection method

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