A gold standard nucleic acid test strip for rapid detection of aquatic diseases and its preparation method

A nucleic acid test strip, aquaculture technology, applied in measurement devices, resistance to vector-borne diseases, instruments, etc., can solve the problems of inseparable from monoclonal antibodies, long production cycle, limited accuracy and reliability, etc. The effect of wide popularization and application, low production cost and simple preparation process
CN109187970BActive Publication Date: 2019-03-08YANTAI TUOPUBANG BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANTAI TUOPUBANG BIOTECH
Publication Date
2019-03-08

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Abstract

The invention relates to a gold-labelled nucleic acid test strip for quickly detecting aquatic diseases. The gold-labelled nucleic acid test strip comprises a sample pad, a gold-labelled pad containing probe Probe1-AuNPs composite, a nitrocellulose membrane and a water absorbent pad; a detection line and a quality control line which are separated from each other are respectively adsorbed on the nitrocellulose membrane; the detection line is composed of a probe2, and the quality control line is composed of the probe 1'; the probe1 and the probe2 are respectively designed by selecting a characteristic conserved segment from a vibrio splendidus toxicity gene sequence and using the 5' end and 3' end of the characteristic conserved segment, and then a complementary sequence Probe1' is designedaccording to the probe1. A nano-gold nucleic acid probe is used as the label in the development of the gold-labelled nucleic acid test strip, and the vibrio splendidus is detected through a thin-filmchromatography measure, thereby providing simple, convenient and efficient detection method for the disease control of the vibrio splendidus.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a gold standard nucleic acid test strip for rapid detection of aquatic diseases and a preparation method thereof. Background technique

[0002] Vibrio splendidus ( Vibrio splendidus ) belongs to the Vibrio family and belongs to the genus Vibrio. It is one of the main pathogenic bacteria of mariculture animals. It can infect hosts such as fish, echinoderms, bivalve molluscs and crustaceans, leading to the occurrence of aquaculture diseases. The rotting skin syndrome of sea cucumbers caused by it can lead to pathological reactions such as mouth swelling, anorexia, body surface ulceration, body atrophy, and viscera discharge, which eventually lead to the death of sea cucumbers and bring huge economic losses to aquaculture. Early detection and diagnosis of the pathogen is an effective means to prevent the occurrence of such diseases. Therefore, it is very important to research and devel...

Claims

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