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Cell wall/cell membrane disruption device and method for using device

A technology for breaking devices and cell membranes, applied in chemical instruments and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve problems such as increased operating costs, achieve easy maintenance and management, reduce operating costs and improve efficiency. Effect

Active Publication Date: 2019-01-11
NIKKAN TOKUSHU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cell walls, etc. constituting microorganisms, etc. are composed of very strong membranes, so in order to break the cell walls, etc., advanced technology and complicated equipment are usually required
Therefore, traditional methods and equipment lead to increased operating costs

Method used

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  • Cell wall/cell membrane disruption device and method for using device
  • Cell wall/cell membrane disruption device and method for using device
  • Cell wall/cell membrane disruption device and method for using device

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0093] use figure 1 (However, 170 is the discharge port, 180 is the input port, and a single-shaft screw pump and a decompression pump are installed at the rear of the discharge port to replace the decompression unit of 190) to treat excess sludge with a cell wall or cell membrane crushing device . The processing condition is that the number of revolutions of the rotating disk is 3500min- 1 , the peripheral speed is 50m / s, the pressure reduction condition is -0.01MPa, and the processing time is 60 minutes. In order to reproduce the anaerobic treatment routinely employed in the field of sewage treatment, a batch test using vials was employed. Anaerobic treatment refers to a biological process in which organic substances contained in sludge are degraded and converted into methane gas through the metabolic action of microorganisms such as methane bacteria under anaerobic anaerobic conditions.

[0094] Put 20 mL of seed sludge (digested sludge) containing methanogens, etc., and...

example 2~ example 5

[0099] (Example 2-Example 5, Comparative Example 2, Comparative Example 3)

[0100] The actual anaerobic treatment takes place continuously. Therefore, in order to carry out a model test of continuous anaerobic treatment, a digester that mixes digested sludge and substrate (excess sludge before treatment with a cell wall or cell membrane disruptor or excess sludge after treatment) and performs anaerobic treatment is used. A test device consisting of a bottle and a produced gas detector that collects the produced gas on the water surface. = 1.5 L of digested sludge as seed sludge was put into a digester bottle (2 L) whose interior was substituted with nitrogen. The operating conditions in the continuous biodegradability test are shown in Table 1. In addition, the digested sludge and substrate used in each operating condition are shown in Table 2. Digested sludge generated from the digestion flasks was sampled daily in the amount reported in Table 1. After sampling, the same...

example 6

[0114] The experimental device for aerobic treatment consists of both an aeration tank and a sedimentation tank. Glucose, which is the input mechanism of the main carbon source, was supplied to the aeration tank under the condition that the inflow BOD was about 200 mg / L. Regarding the operating conditions, the activated sludge put into the aeration tank is 20L, the residence time is 24 hours, and the MLSS in the aeration tank is set at 1,000-2,000mg / L. The days of consecutive trials were 20 days. After concentrating the excess sludge generated in this process, the thing treated with the cell wall or cell membrane crushing device is returned to the aeration tank, and the amount of excess sludge generated, as well as the water quality in the aeration tank and the treated water are studied. The treatment conditions of the cell wall or cell membrane disruption device were implemented under the same conditions as in Example 1. The result is in Figure 6 , shown in Table 4 and Ta...

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PUM

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Abstract

Provided is a device for disrupting cell walls and / or cell membranes of microorganisms, algae, etc., included in organic sludge etc., the cell wall / cell membrane disruption device comprising fixed disks, rotary disks, a rotary shaft for driving said rotary disks, a depressurizing means, and a housing, wherein: at least one set of said fixed disk and said rotary disk is arranged so as to oppose oneanother; a central section of said fixed disk has a hollow section that is larger than the outer diameter of the rotary shaft passing through the central section; a shearing force produced between said rotary disks and said fixed disks is applied to a target fluid introduced inside the device and having a water content of 89% or higher; and the pressure inside the cell wall / cell membrane disruption device is reduced to -0.08 MPa or less by the depressurizing means. This device can contribute to an increase in the amount of biogas, a reduction in the amount of sludge, culturing of algae, plantcultivation, culturing of aquatic products, etc., and can separate, for example, CH4 and CO2 and make them into resources.

Description

technical field [0001] The present invention relates to a device for disrupting cell walls and / or cell membranes of microorganisms, algae, etc., and a method for using the device. Background technique [0002] Microorganisms, algae, etc. contained in organic sludge etc. contain various useful resources (for example, protein, fat, carbohydrate). However, in order to recover such useful resources, it is necessary to crush cell walls and / or cell membranes (hereinafter, also referred to as "cell walls and the like") constituting microorganisms and the like. Cell walls and the like constituting microorganisms and the like are composed of very strong membranes, and therefore advanced technology and complicated equipment are usually required to disrupt the cell walls and the like. Therefore, conventional methods and equipment lead to increased operating costs. [0003] For example, Patent Document 1 discloses a sludge crushing device, which is a sludge crushing device for crushin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/33B02C7/02B02C19/18C02F11/00C02F11/04
CPCB02C7/02C02F11/00C02F11/04C12M1/33Y02W10/37B02C19/18C02F2201/003C02F2303/02C02F2303/26Y02E50/30Y02W30/40C02F11/12C12M45/02Y02A40/20A23L5/30A23V2002/00C12N1/066
Inventor 梅田洪日
Owner NIKKAN TOKUSHU CO LTD