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Domestic fungus strain preservation medium and application and preservation method thereof

A technology of edible fungi and culture medium, applied in the field of microorganisms, can solve the problems of loss of high-yield traits, reduction of metabolic function, loss of physiological functions, etc., and achieve the goal of reducing the degradation rate of bacteria, maintaining the growth rate of mycelia, and maintaining vitality Effect

Inactive Publication Date: 2019-01-15
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main reason for the degradation of the strains is that when the strains are artificially cultivated, the cultivation conditions are not suitable (external factors), including nutrition, temperature, humidity, ventilation, pH value, etc., which cannot meet its living requirements, so that the edible fungi lose their self-regulation. the ability to temporarily lose the normal physiological function, unable to express the good breed
As far as a certain strain is concerned, with the prolongation of cultivation and use time, the age of the individual is getting older, the metabolic function is gradually reduced, the ability to resist stress is lost, or the high-yield traits are lost, until it loses its use value

Method used

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  • Domestic fungus strain preservation medium and application and preservation method thereof
  • Domestic fungus strain preservation medium and application and preservation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: preservation medium of the present invention

[0023] Kenaf bone 10%, cottonseed hull 60.99%, wheat bran 21%, corn flour 4%, sucrose 2%, calcium carbonate 2% and biotin 0.01%; or

[0024] Kenaf bone 10%, cottonseed hull 50.99%, wheat bran 31%, corn flour 4%, sucrose 2%, calcium carbonate 2% and biotin 0.01%.

Embodiment 2

[0025] Embodiment 2: Different preservation culture medium comparative test

[0026] 1. Test medium

[0027] Medium No. 1: kenaf bone 10%, cottonseed hull 60.99%, wheat bran 21%, corn flour 4%, sucrose 2%, calcium carbonate 2% and biotin 0.01%;

[0028] Medium No. 2: kenaf bone 10%, cottonseed hull 50.99%, wheat bran 31%, corn flour 4%, sucrose 2%, calcium carbonate 2% and biotin 0.01%;

[0029] Medium No. 3: kenaf bone 20%, cottonseed hull 50.99%, wheat bran 21%, corn flour 4%, sucrose 2%, calcium carbonate 2% and vitamin B 1 0.01%;

[0030] Medium No. 4: kenaf bone 20%, cottonseed hull 40.99%, wheat bran 31%, corn flour 4%, sucrose 2%, calcium carbonate 2% and vitamin B 1 0.01%;

[0031] No. 5 medium (conventional medium): 70.99% cottonseed hulls, 21% wheat bran, 4% corn flour, 2% sucrose, 2% calcium carbonate, vitamin B 1 0.01% and biotin 0.01%;

[0032] The medium of each group was divided into 40 bags, each bag had the same total weight, inoculated with the same ...

Embodiment 3

[0038] Embodiment 3: the verification of the preservation medium component effect of the present invention

[0039] On the basis of No. 1 and No. 2 medium in Example 2, vitamin B was added 1 And increase the consumption of kenaf hemp bone, form following four kinds of contrast culture medium:

[0040] Medium A: kenaf bone 10%, cottonseed hull 60.99%, wheat bran 21%, corn flour 4%, sucrose 2%, calcium carbonate 2%, vitamin B 1 0.01% and biotin 0.01%;

[0041] B medium: kenaf bone 10%, cottonseed hull 50.99%, wheat bran 31%, corn flour 4%, sucrose 2%, calcium carbonate 2%, vitamin B 1 0.01% and biotin 0.01%;

[0042] C medium: kenaf bone 20%, cottonseed hull 50.99%, wheat bran 21%, corn flour 4%, sucrose 2%, calcium carbonate 2% and biotin 0.01%;

[0043] D medium: kenaf bone 20%, cottonseed hull 40.99%, wheat bran 31%, corn flour 4%, sucrose 2%, calcium carbonate 2% and biotin 0.01%;

[0044] According to the method of embodiment 2, the degeneration rate of bacterial speci...

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Abstract

The invention discloses a domestic fungus strain preservation medium and application and preservation method thereof, and relates to the technical field of microbe. The medium comprise kenaf fiber stalks, cottonseed husks, wheat bran, corn flour, cane sugar, calcium carbonate and biotin and does not include vitamin B1. The kenaf fiber stalks and the biotin are added to and vitamin B1 is deleted from a routine medium to form the new domestic fungus strain preservation medium, the problem of relatively high spawn degeneration rate due to multitime passage can be solved, and the mycelia growth speed of the strains is kept in a relatively high level.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for preserving edible fungus strains and its application and preservation method. Background technique [0002] Long-term use, storage, and too many transfer times of edible fungus strains will lead to a decline in mycelial viability. During the propagation of the strains, it is often found that some original good traits gradually disappear or deteriorate, and the growth is weak. Slow growth, late fruiting, low yield, poor quality and other changes. These phenomena are called "degeneration". [0003] The main reason for the degradation of the strains is that when the strains are artificially cultivated, the cultivation conditions are not suitable (external factors), including nutrition, temperature, humidity, ventilation, pH value, etc., which cannot meet its living requirements, so that the edible fungi lose their self-regulation. Ability, so that tempo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 彭源德谢纯良朱作华龚文兵周映君严理胡镇修
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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