A method for extracting and separating and purifying polysaccharide from myrtle fruit

A technology for separation and purification of myrtle, applied in the field of extracting and separating and purifying polysaccharides from myrtle fruit, can solve the problems of low level of processing and utilization, low utilization rate of development, etc. Low, high yield effect

Active Publication Date: 2021-05-07
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Myrtle (Rhodomyrtus tomentosa) is also known as Gangren (Guangdong), Doumingan (Guangxi), Shidu Nianzi ("Supplement to Materia Medica"), Daomanzi ("Lingbiao Luyi"), Daonianzi ("Su Shen Liangfang") 》), etc., widely distributed in the subtropical region; it is the dominant species in the evergreen broad-leaved forest ecosystem, with a coverage of 30% to 60%, and it is a pioneer community; it is mainly distributed in the South of the Five Ridges in my country, and is the main undergrowth in southern China Vegetation, resources are very rich, mostly born in hilly slopes, wilderness, roadside, and wild myrtle is widely distributed, but its development and utilization rate is low at present, and the level of processing and utilization is relatively low, myrtle belongs to a A potential new food resource that has not yet been developed and utilized on a large scale

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  • A method for extracting and separating and purifying polysaccharide from myrtle fruit
  • A method for extracting and separating and purifying polysaccharide from myrtle fruit
  • A method for extracting and separating and purifying polysaccharide from myrtle fruit

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Embodiment 1

[0042] A method for extracting and isolating and purifying polysaccharides from myrtle fruit, comprising the following steps:

[0043] (1) Extract polysaccharides from myrtle fruit: Weigh 110g of dried myrtle fruit and crush them, pass through a 40-mesh sieve, weigh 100g of dry powder, add 1000mL of distilled water, boil in a reflux device for 4h, and take the filter residue after suction filtration Then add 1000 mL of distilled water, boil for 4 h in a reflux device, combine the supernatants, and concentrate under reduced pressure. Slowly add 4 times the volume of 95% (V / V) ethanol to the concentrated solution, and keep stirring with a glass rod to uniformly precipitate the polysaccharide, then put it in a refrigerator at 4°C for 12 hours, and then centrifuge at 5000r / min for 15 minutes to collect the precipitate. After drying, myrtle fruit crude polysaccharide is obtained;

[0044] (2)H 2 o 2 Decolorization method: Weigh 20g myrtle fruit crude polysaccharide, add 100mL di...

Embodiment 2

[0054] The myrtle fruit polysaccharides obtained in Example 1 were subjected to ultraviolet spectrum analysis. Each 1 mg polysaccharide sample was weighed to prepare a 1 mg / mL polysaccharide solution, and the ultraviolet spectrum was scanned in the range of 200-500 nm.

[0055] figure 2 is the UV spectrum of P1, image 3 is the UV spectrum of P2, Figure 4 is the UV spectrum of P3, Figure 5 It is the ultraviolet spectrogram of P4, and the results show that there are no obvious absorption peaks at 260nm and 280nm in the four purified components, indicating that they do not contain protein and nucleic acid substances.

Embodiment 3

[0057] Carry out polysaccharide molecular weight analysis to the myrtle fruit polysaccharide that embodiment 1 obtains, concrete experimental method is as follows:

[0058] Molecular weights were determined using gel permeation chromatography (GPC). Take 2 mg of polysaccharide sample, add 0.02 mol / L phosphate buffer solution to dissolve, prepare 2.0 mg / mL solution, filter with 0.22 μm sterile filter membrane, and take the filtrated liquid for later use. Chromatographic conditions: column temperature 35°C, 0.02mol / L phosphate buffer as mobile phase, flow rate 0.6mL / min, injection volume 20μL, Waters 2410 differential refractive index detector for detection. Prepare a series of dextran solutions with different molecular weights (700, 400, 200, 100, 50, 30, 10, 5kD) as standards, draw a standard curve, and calculate the molecular weight of the sample according to its corresponding elution volume against the standard curve .

[0059] The measured molecular weight of the myrtle f...

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Abstract

The invention discloses a method for extracting and separating and purifying polysaccharides from myrtle fruit. The method comprises the following steps: reflux extracting the myrtle fruit to obtain the myrtle fruit crude polysaccharide, and then adding 30% H 2 o 2 The solution is decolorized; the decolorized polysaccharide is added with Sevag reagent to remove the protein, and the polysaccharide of myrtle fruit is initially purified; it is eluted with a gradient concentration of NaCl solution, the eluate is collected, and the eluate is detected by the phenol-sulfuric acid method at a detection wavelength of According to the absorbance value at 490nm, the same fractions were combined according to the elution curve, concentrated, dialyzed, and freeze-dried to obtain polysaccharides P1, P2, P3, and P4, respectively. The present invention establishes a complete and feasible technical route for the extraction, separation and purification of myrtle fruit polysaccharide, structural characteristics and biological activity research, and provides technical guidance for the extraction, separation and purification of the wild plant resource myrtle fruit polysaccharide.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying polysaccharide from myrtle fruit. Background technique [0002] Myrtle (Rhodomyrtus tomentosa) is also known as Gangren (Guangdong), Doumingan (Guangxi), Shidu Nianzi ("Supplement to Materia Medica"), Daomanzi ("Lingbiao Luyi"), Daonianzi ("Su Shen Liangfang") 》), etc., widely distributed in the subtropical region; it is the dominant species in the evergreen broad-leaved forest ecosystem, with a coverage of 30% to 60%, and it is a pioneer community; it is mainly distributed in the South of the Five Ridges in my country, and is the main undergrowth in southern China Vegetation, resources are very rich, mostly born in hilly slopes, wilderness, roadside, and wild myrtle is widely distributed, but its development and utilization rate is low at present, and the level of processing and utilization is relatively low, myrtle belongs to a A potential new food resource that has not yet been...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 黄儒强王静辉王倩高林林张竞雯
Owner SOUTH CHINA NORMAL UNIVERSITY
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