Construction of Lactobacillus curvularius ATCC55221 and application thereof in treatment of parturient nipple rhagadia

A technology of ATCC55221, Lactobacillus crispatus, applied in the field of biopharmaceuticals, can solve problems such as the inability to meet tissue repair

Inactive Publication Date: 2019-01-18
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, CXCL12 is easily degraded by dipeptide aminopeptidase IV (DPP IV) in human blood, and the level of endogenous CXCL12 produced during the repair of severe tissue damage cannot meet the needs of tissue repair, so it is necessary to introduce exogenous CXCL12

Method used

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  • Construction of Lactobacillus curvularius ATCC55221 and application thereof in treatment of parturient nipple rhagadia
  • Construction of Lactobacillus curvularius ATCC55221 and application thereof in treatment of parturient nipple rhagadia
  • Construction of Lactobacillus curvularius ATCC55221 and application thereof in treatment of parturient nipple rhagadia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Establishment of mouse nipple chapped model

[0062] (1) The experimental groups were divided into negative control group (group C), model group (group M), low-dose treatment group (LT group) of engineering strains of Lactobacillus crispatus, and high-dose treatment group (group HT) of engineering strains of Lactobacillus crispatus. Group of 8;

[0063] (2) Take 32 Balb / c mice, numbered 1-32 at random. No food or water was allowed 12 hours before the operation. The mice were fixed with a fixed frame, and the hair on both sides of the back of the experimental site was roughly cut off with an automatic shaving machine close to the skin, and then the fine hair was removed with a depilatory cream. Wash thoroughly with warm water immediately after epilation, leaving skin bare for later use. On the day of operation, they were anesthetized by intraperitoneal injection of 3.6% chloral hydrate 10mL / kg. Immerse the metal disc in liquid nitrogen at -196°C, and immediately s...

Embodiment 2

[0068] 1. Appearance evaluation of chapped nipple model in animal experiments after treatment

[0069] 1. Appearance chapped degree score

[0070] The scoring criteria were evaluated based on the depth of the wound and the degree of tissue damage, and the scores were all evaluated by an experimenter who was blind to the experiment. The higher the score, the more severe the degree of chapping; 0-1 points represent normal or healed. See the table below for the scoring criteria of the degree of chapping.

[0071] Rating criteria for the degree of chapping

[0072]

[0073]

Embodiment 3

[0075] 1. Histopathological detection of chapped nipple model in animal experiments after treatment

[0076] The evaluation was carried out from three aspects: regeneration of epidermis and dermis, thickness of granulation tissue, and angiogenesis. HE staining was used to observe the pathological changes of the wound. Masson staining was used to observe the repair of skin scar after injury.

[0077] 1. Histological score - HE staining of wound tissue

[0078] (1) Dehydration. Take out the wound tissue that has been fixed, rinse it well with PBS, and then dehydrate with ethanol, respectively with 70% ethanol, 80% ethanol, 90% ethanol, 95% ethanol, and 95% ethanol for 150 minutes; then dehydrate with absolute ethanol twice , respectively 60min;

[0079] (2) Embedding. The dehydrated tissues were treated with xylene for 30 minutes, and embedded in paraffin;

[0080] (3) Slicing and sealing. Cut out a flat plane with a rotary machine, set the thickness of the section to 55 ...

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Abstract

Provided are construction of Lactobacillus curvularius ATCC 55221 and application thereof in treatment of parturient nipple rhagadia. First, the human and mouse chemokine CXCL12, also known as stromalcell-derived factor-1 (SDF-1), is a small molecule protein belonging to the CXC chemokine family. The secretory expression transmembrane peptide SPusp45, which is unique to Lactococcus lactis, was added to construct a recombinant plasmid pMG36e-CXCL12, which was then electroporated into the Lactobacillus crispus ATCC 55221 to achieve the secretory expression of the chemokine CXCL12. By external application of nipple wound application, on the one hand, the direct secretion of chemokine CXCL12 can achieve rapid repair of nipple wounds; on the other hand, infants or newborns can obtain probioticsupplements by direct sucking, helping infants Or the newborn balances the intestinal flora to improve autoimmunity.

Description

technical field [0001] The invention relates to a preparation method of Lactobacillus crispatus ATCC 55221 secreting and expressing chemokine CXCL12 and its application in chapped nipples of parturient women, belonging to the field of biopharmaceuticals. Background technique [0002] Breast milk is the most ideal natural food for infants, and breastfeeding is beneficial to both infants and mothers in the short and long term. However, the exclusive breastfeeding rate in the first 6 months of life in the world is only 37%. There are many factors that affect the early exclusive breastfeeding rate, and the most common complaints are nipple pain and chapped. Cracked nipple pain is one of the main influencing factors for early abandonment of exclusive breastfeeding, and it is also one of the main reasons for early weaning. [0003] Cracked nipples are one of the common diseases during breastfeeding. The high incidence period is 24-72 hours after delivery. In mild cases, only crac...

Claims

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Application Information

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IPC IPC(8): C12N15/74A61K35/747A61K38/19A61P15/14
CPCA61K38/195A61K2035/115A61P15/14C12N15/74
Inventor 陈廷涛田溥塬
Owner NANCHANG UNIV
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