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Self assembly polypeptide hydrogel scaffold and preparation method thereof

A hydrogel and self-assembly technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of difficult tissue regeneration, loss, and reduced growth activity of cultured cells, so as to maintain chondrocyte phenotype and promote differentiation role, the effect of promoting deposition

Pending Publication Date: 2019-01-22
南京汉瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The micropores between the 3D printed scaffold fibers range from 10 to 700 microns (cross section), usually 1000 to 100,000 times the size of biomolecules, so these biomolecules, nutrients such as vitamins, amino acids, proteins or drugs will Rapid loss during the exchange process of scaffolds and body fluids, resulting in reduced growth activity of cells cultured on scaffolds in vitro and difficulties in tissue regeneration in vivo

Method used

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  • Self assembly polypeptide hydrogel scaffold and preparation method thereof
  • Self assembly polypeptide hydrogel scaffold and preparation method thereof
  • Self assembly polypeptide hydrogel scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. 3D printing PCL skeleton:

[0053] FDM rapid extrusion molding, 3D printing heating system heats up to 100 ℃ PCL particles (molecular weight 10000~80000) become viscous fluid, extruded fiber bundles (diameter range 300~500 microns), through different layers of fiber bundles A spliced ​​architecture with angles to obtain a 3D printed skeleton. Each layer of fibers intersects and accumulates in the range of 0~180° to form a triangular structure of 0~60~120°, with a skeleton pore size of 500 microns and a porosity of more than 85%.

[0054] 2. Preparation of polypeptide self-assembled hydrogel-sol with FE and FK structural units:

[0055] Dissolve FEFEFKFK self-assembled polypeptide (SAP) in double-distilled water to make acidic sol-gel, vortex ultrasonically, heat at 80°C for 1 hour to ensure complete dissolution, and prepare concentrations of 0.5%wt, 1.5%wt and 3.0%wt respectively peptide hydrogels.

[0056] 3. Self-assembled hydrogel-coated PCL scaffolds

[0057]...

Embodiment 2

[0067] The difference between Example 2 and Example 1 is that PLA with a molecular weight of 5,000-40,000 is used for the backbone, and the concentration of the prepared polypeptide hydrogel is 2.0%wt, and the polypeptide used is FEFKFEFK.

Embodiment 3

[0069] The difference between Example 3 and Example 1 is that the backbone part uses PLGA with a molecular weight of 4000-10000, and the concentration of the prepared polypeptide hydrogel is 4.0%wt, and the polypeptide used is FEFEFKFKFEFEFKFK (FEK16).

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Abstract

The invention discloses a self assembly polypeptide hydrogel scaffold, which comprises a framework and self assembly polypeptide hydrogel modified on the surface of the framework, wherein the framework is made of materials of high-molecular polymers with hydrophobic surfaces; and polypeptide is the polypeptide with FE and FK structure units. The self assembly polypeptide hydrogel scaffold has excellent rigidity and surface biocompatibility at the same time, can promote the osteogenic differentiation of mesenchymal stem cells and the phenotype maintenance in the chondrocyte self differentiationprocess, and realizes the bone and cartilage layered regeneration in an osteochondral defect model.

Description

technical field [0001] The invention belongs to the field of bone tissue repair and reconstruction, and in particular relates to a self-assembled polypeptide hydrogel tissue engineering scaffold and a preparation method thereof. Background technique [0002] The human body's movement system is mainly composed of bones, joints and skeletal muscles. As a movement hub, joints play an important role in the body's actions, including buffering stress, transmitting loads, reducing friction, etc., which determine the complex and fine structure of joints. . With the aging of the population and the increasing obesity, the number of people suffering from arthritis is increasing every year. According to statistics, 250,000 people in China need knee and hip replacements every year, so the repair of joint damage has been in recent years. is a research hotspot. At present, clinical treatment methods for articular cartilage repair mainly include microfracture, autologous cartilage transpl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/56A61L27/22
CPCA61L27/227A61L27/52A61L27/56A61L2430/06A61L2430/02
Inventor 柳丽朱颐申姚庆强李岚王黎明王谷丰
Owner 南京汉瑞生物科技有限公司
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